Research Output 1987 2019

2019

GPAT2 is required for piRNA biogenesis, transposon silencing, and maintenance of spermatogonia in mice†

Shiromoto, Y., Kuramochi-Miyagawa, S., Nagamori, I., Chuma, S., Arakawa, T., Nishimura, T., Hasuwa, H., Tachibana, T., Ikawa, M. & Nakano, T., 2019 Jul 1, In : Biology of reproduction. 101, 1, p. 248-256 9 p.

Research output: Contribution to journalArticle

Open Access
Acyltransferases
Spermatogonia
Retroelements
Small Interfering RNA
Spermatogenesis

Nuclear RNA export factor variant initiates piRNA-guided co-transcriptional silencing

Murano, K., Iwasaki, Y., Ishizu, H., Mashiko, A., Shibuya, A., Kondo, S., Adachi, S., Suzuki, S., Saito, K., Natsume, T., Siomi, M. C. & Siomi, H., 2019 Jan 1, In : EMBO Journal. e102870.

Research output: Contribution to journalArticle

Nuclear RNA
Cell Nucleus Active Transport
DNA Transposable Elements
Heterochromatin
RNA
1 Citation (Scopus)

Requirements for multivalent Yb body assembly in transposon silencing in Drosophila

Hirakata, S., Ishizu, H., Fujita, A., Tomoe, Y. & Siomi, M. C., 2019 Jan 1, In : EMBO Reports. e47708.

Research output: Contribution to journalArticle

RNA Helicases
Drosophila
RNA
Liquids
2018
1 Citation (Scopus)

Constitutive overexpression of periostin delays wound healing in mouse skin

Nunomura, S., Nanri, Y., Ogawa, M., Arima, K., Mitamura, Y., Yoshihara, T., Hasuwa, H., Conway, S. J. & Izuhara, K., 2018 Jan 1, (Accepted/In press) In : Wound Repair and Regeneration.

Research output: Contribution to journalArticle

Wound Healing
Skin
Transgenic Mice
Wounds and Injuries
Interleukin-1

Erratum: Publisher Correction: Female mice lacking Ftx lncRNA exhibit impaired X-chromosome inactivation and a microphthalmia-like phenotype (Nature communications (2018) 9 1 (3829))

Hosoi, Y., Soma, M., Shiura, H., Sado, T., Hasuwa, H., Abe, K., Kohda, T., Ishino, F. & Kobayashi, S., 2018 Oct 31, In : Nature Communications. 9, 1, 1 p.

Research output: Contribution to journalComment/debate

Long Noncoding RNA
document markup languages
Microphthalmos
X Chromosome Inactivation
phenotype
2 Citations (Scopus)

Female mice lacking Ftx lncRNA exhibit impaired X-chromosome inactivation and a microphthalmia-like phenotype

Hosoi, Y., Soma, M., Shiura, H., Sado, T., Hasuwa, H., Abe, K., Kohda, T., Ishino, F. & Kobayashi, S., 2018 Dec 1, In : Nature Communications. 9, 1, 3829.

Research output: Contribution to journalArticle

Long Noncoding RNA
Microphthalmos
X Chromosome Inactivation
phenotype
chromosomes
3 Citations (Scopus)

Hepatic Ago2-mediated RNA silencing controls energy metabolism linked to AMPK activation and obesity-associated pathophysiology

Zhang, C., Seo, J., Murakami, K., Salem, E. S. B., Bernhard, E., Borra, V. J., Choi, K., Yuan, C. L., Chan, C. C., Chen, X., Huang, T., Weirauch, M. T., Divanovic, S., Qi, N. R., Thomas, H. E., Mercer, C. A., Siomi, H. & Nakamura, T., 2018 Dec 1, In : Nature Communications. 9, 1, 3658.

Research output: Contribution to journalArticle

obesity
AMP-Activated Protein Kinases
metabolism
RNA Interference
Energy Metabolism
9 Citations (Scopus)

Hierarchical roles of mitochondrial Papi and Zucchini in Bombyx germline piRNA biogenesis

Nishida, K. M., Sakakibara, K., Iwasaki, Y., Yamada, H., Murakami, R., Murota, Y., Kawamura, T., Kodama, T., Siomi, H. & Siomi, M. C., 2018 Mar 8, In : Nature. 555, 7695, p. 260-264 5 p.

Research output: Contribution to journalArticle

Bombyx
Small Interfering RNA
RNA
Exonucleases
Endonucleases
1 Citation (Scopus)

Laminin γ1 c-terminal Glu to Gln mutation induces early postimplantation lethality

Kiyozumi, D., Taniguchi, Y., Nakano, I., Toga, J., Yagi, E., Hasuwa, H., Ikawa, M. & Sekiguchi, K., 2018 Jan 1, In : Life Science Alliance. 1, 5, e201800064.

Research output: Contribution to journalArticle

laminin
integrins
Integrins
mutation
Mutation
7 Citations (Scopus)

Piwi Nuclear Localization and Its Regulatory Mechanism in Drosophila Ovarian Somatic Cells

Yashiro, R., Murota, Y., Nishida, K. M., Yamashiro, H., Fujii, K., Ogai, A., Yamanaka, S., Negishi, L., Siomi, H. & Siomi, M. C., 2018 Jun 19, In : Cell Reports. 23, 12, p. 3647-3657 11 p.

Research output: Contribution to journalArticle

Karyopherins
Nuclear Localization Signals
Drosophila
RNA
Genes

Profiling open chromatin structure in the ovarian somatic cells using ATAC-seq

Murano, K., Iwasaki, Y. & Siomi, H., 2018, Methods in Molecular Biology. Humana Press Inc., Vol. 1680. p. 165-177 13 p. (Methods in Molecular Biology; vol. 1680).

Research output: Chapter in Book/Report/Conference proceedingChapter

Transposases
Chromatin
DNA Transposable Elements
Deoxyribonucleases
Heterochromatin
7 Citations (Scopus)

Tbx6 Induces Nascent Mesoderm from Pluripotent Stem Cells and Temporally Controls Cardiac versus Somite Lineage Diversification

Sadahiro, T., Isomi, M., Muraoka, N., Kojima, H., Haginiwa, S., Kurotsu, S., Tamura, F., Tani, H., Tohyama, S., Fujita, J., Miyoshi, H., Kawamura, Y., Goshima, N., Iwasaki, Y., Murano, K., Saito, K., Oda, M., Andersen, P., Kwon, C., Uosaki, H. & 3 others, Nishizono, H., Fukuda, K. & Ieda, M., 2018 Sep 6, In : Cell Stem Cell. 23, 3, p. 382-395 14 p.

Research output: Contribution to journalArticle

Somites
Pluripotent Stem Cells
Mesoderm
Clustered Regularly Interspaced Short Palindromic Repeats
Germ Layers

Vwf K1362A resulted in failure of protein synthesis in mice

Sanda, N., Suzuki, N., Suzuki, A., Kanematsu, T., Kishimoto, M., Hasuwa, H., Takagi, A., Kojima, T., Matsushita, T. & Nakamura, S., 2018 Apr 1, In : International Journal of Hematology. 107, 4, p. 428-435 8 p.

Research output: Contribution to journalArticle

von Willebrand Factor
Proteins
Platelet Glycoprotein GPIb-IX Complex
Platelet Membrane Glycoproteins
Factor VIII
2017
3 Citations (Scopus)

Deep sequencing and high-throughput analysis of PIWI-associated small RNAs

Iwasaki, Y., Ishino, K. & Siomi, H., 2017 Mar 28, (Accepted/In press) In : Methods.

Research output: Contribution to journalArticle

High-Throughput Nucleotide Sequencing
Throughput
RNA
Testis
Regulator Genes
1 Citation (Scopus)

Human PIWI (HIWI) is an azoospermia factor

Hasuwa, H., Ishino, K. & Siomi, H., 2017 Aug 10, (Accepted/In press) In : Science China Life Sciences. p. 1-3 3 p.

Research output: Contribution to journalArticle

Argonaute Proteins
Biological Models
Azoospermia
Spermatogenesis
spermatogenesis
1 Citation (Scopus)

Identification of mouse piRNA pathway components using anti-MIWI2 antibodies

Hirano, T., Hasuwa, H. & Siomi, H., 2017, Methods in Molecular Biology. Humana Press Inc., Vol. 1463. p. 205-216 12 p. (Methods in Molecular Biology; vol. 1463).

Research output: Chapter in Book/Report/Conference proceedingChapter

Epigenomics
Small Interfering RNA
Anti-Idiotypic Antibodies
RNA
Biological Phenomena
8 Citations (Scopus)

Mitotic phosphorylation of CCCTC-binding factor (CTCF) reduces its DNA binding activity

Sekiya, T., Murano, K., Kato, K., Kawaguchi, A. & Nagata, K., 2017 Mar 1, In : FEBS Open Bio. 7, 3, p. 397-404 8 p.

Research output: Contribution to journalArticle

Phosphorylation
Mitosis
Chromatin
Chromosomes
Zinc

Mobile elements control stem cell potency

Hasuwa, H. & Siomi, H., 2017 Feb 10, In : Science. 355, 6325, p. 581-582 2 p.

Research output: Contribution to journalShort survey

cells and cell components
element mobility
DNA Transposable Elements
genetic analysis
rodent
2016
15 Citations (Scopus)

Biogenesis of sperm acrosome is regulated by pre-mRNA alternative splicing of acrbp in the mouse

Kanemori, Y., Koga, Y., Sudo, M., Kang, W., Kashiwabara, S. I., Ikawa, M., Hasuwa, H., Nagashima, K., Ishikawa, Y., Ogonuki, N., Ogura, A. & Baba, T., 2016 Jun 28, In : Proceedings of the National Academy of Sciences of the United States of America. 113, 26, p. E3696-E3705

Research output: Contribution to journalArticle

Acrosome
RNA Precursors
Alternative Splicing
Spermatozoa
Spermatids
35 Citations (Scopus)

Crystal Structure of Silkworm PIWI-Clade Argonaute Siwi Bound to piRNA

Matsumoto, N., Nishimasu, H., Sakakibara, K., Nishida, K. M., Hirano, T., Ishitani, R., Siomi, H., Siomi, M. C. & Nureki, O., 2016 Oct 6, In : Cell. 167, 2, p. 484-497.e9

Research output: Contribution to journalArticle

Bombyx
Guide RNA
Small Interfering RNA
Crystal structure
Argonaute Proteins
7 Citations (Scopus)

Impaired female fertility in tubulointerstitial Antigen-Like 1-Deficient mice

Takahashi, A., Rahim, A., Takeuchi, M., Fukui, E., Yoshizawa, M., Mukai, K., Suematsu, M., Hasuwa, H., Okabe, M. & Matsumoto, H., 2016 Feb 20, In : Journal of Reproduction and Development. 62, 1, p. 43-49 7 p.

Research output: Contribution to journalArticle

female fertility
pregnancy
antigens
endometrium
mice
26 Citations (Scopus)

Inheritance of a Nuclear PIWI from Pluripotent Stem Cells by Somatic Descendants Ensures Differentiation by Silencing Transposons in Planarian

Shibata, N., Kashima, M., Ishiko, T., Nishimura, O., Rouhana, L., Misaki, K., Yonemura, S., Saito, K., Siomi, H., Siomi, M. C. & Agata, K., 2016 May 9, In : Developmental Cell. 37, 3, p. 226-237 12 p.

Research output: Contribution to journalArticle

Planarians
Pluripotent Stem Cells
Stem cells
Transcription
RNA
17 Citations (Scopus)

Loss of l(3)mbt leads to acquisition of the ping-pong cycle in Drosophila ovarian somatic cells

Sumiyoshi, T., Sato, K., Yamamoto, H., Iwasaki, Y., Siomi, H. & Siomi, M. C., 2016 Jul 15, In : Genes and Development. 30, 14, p. 1617-1622 6 p.

Research output: Contribution to journalArticle

Brain Neoplasms
Drosophila
Clustered Regularly Interspaced Short Palindromic Repeats
Menstrual Cycle
Germ Cells
11 Citations (Scopus)

MIWI2 as an Effector of DNA Methylation and Gene Silencing in Embryonic Male Germ Cells

Kojima-Kita, K., Kuramochi-Miyagawa, S., Nagamori, I., Ogonuki, N., Ogura, A., Hasuwa, H., Akazawa, T., Inoue, N. & Nakano, T., 2016 Sep 13, In : Cell Reports. 16, 11, p. 2819-2828 10 p.

Research output: Contribution to journalArticle

Gene Silencing
DNA Methylation
Genes
Cells
Zinc Fingers
2015

Changes in bacterial community structure associated with phytoplankton succession in outdoor experimental ponds

Kobayashi, Y., Hodoki, Y., Ohbayashi, K., Okuda, N. & Nakano, S. I., 2015 Mar 20, In : Plankton and Benthos Research. 10, 1, p. 34-44 11 p.

Research output: Contribution to journalArticle

bacterial communities
algae
Microcystis
community structure
pond
18 Citations (Scopus)

Crystal structure and activity of the endoribonuclease domain of the piRNA pathway factor maelstrom

Matsumoto, N., Sato, K., Nishimasu, H., Namba, Y., Miyakubi, K., Dohmae, N., Ishitani, R., Siomi, H., Siomi, M. C. & Nureki, O., 2015 Apr 21, In : Cell Reports. 11, 3, p. 366-375 10 p.

Research output: Contribution to journalArticle

Endoribonucleases
Small Interfering RNA
Ribonuclease H
Crystal structure
RNA
7 Citations (Scopus)

Gene expression ontogeny of spermatogenesis in the marmoset uncovers primate characteristics during testicular development

Lin, Z. Y. C., Hirano, T., Shibata, S., Seki, N. M., Kitajima, R., Sedohara, A., Siomi, M. C., Sasaki, E., Siomi, H., Imamura, M. & Okano, H., 2015 Apr 1, In : Developmental Biology. 400, 1, p. 43-58 16 p.

Research output: Contribution to journalArticle

Callithrix
Spermatogenesis
Primates
Gene Expression
Spermatogonia
2 Citations (Scopus)

Identification of species and genotypic compositions of Cryptomonas (Cryptophyceae) populations in the eutrophic Lake Hira, Japan, using single-cell PCR

Nishino, H., Hodoki, Y., Thottathil, S. D., Ohbayashi, K., Takao, Y. & Nakano, S. I., 2015 Sep 28, In : Aquatic Ecology. 49, 3, p. 263-272 10 p.

Research output: Contribution to journalArticle

Cryptophyceae
Cryptomonas
Japan
lakes
lake
26 Citations (Scopus)

Krimper Enforces an Antisense Bias on piRNA Pools by Binding AGO3 in the Drosophila Germline

Sato, K., Iwasaki, Y., Shibuya, A., Carninci, P., Tsuchizawa, Y., Ishizu, H., Siomi, M. C. & Siomi, H., 2015 Aug 20, In : Molecular Cell. 59, 4, p. 553-563 11 p.

Research output: Contribution to journalArticle

Small Interfering RNA
Drosophila
Solanum melongena
Antisense RNA
Ovary
4 Citations (Scopus)

Misprocessed tRNA response targets piRNA clusters

Yamanaka, S. & Siomi, H., 2015 Dec 14, In : EMBO Journal. 34, 24, p. 2988-2989 2 p.

Research output: Contribution to journalArticle

Transcription
Transfer RNA
RNA
Mutation
Drosophila melanogaster
11 Citations (Scopus)

Phased piRNAs tackle transposons

Siomi, H. & Siomi, M. C., 2015 May 15, In : Science. 348, 6236, p. 756-757 2 p.

Research output: Contribution to journalArticle

Argonaute Proteins
Guide RNA
Endoribonucleases
Genetic Transcription
RNA Cleavage
24 Citations (Scopus)

PiRNAs derived from ancient viral processed pseudogenes as transgenerational sequence-specific immune memory in mammals

Parrish, N. F., Fujino, K., Shiromoto, Y., Iwasaki, Y., Ha, H., Xing, J., Makino, A., Kuramochi-Miyagawa, S., Nakano, T., Siomi, H., Honda, T. & Tomonaga, K., 2015 Oct 1, In : RNA. 21, 10, p. 1691-1703 13 p.

Research output: Contribution to journalArticle

Bornaviridae
Pseudogenes
Nucleoproteins
Mammals
RNA
209 Citations (Scopus)

PIWI-interacting RNA: Its biogenesis and functions

Iwasaki, Y., Siomi, M. C. & Siomi, H., 2015 Jun 2, In : Annual Review of Biochemistry. 84, p. 405-433 29 p.

Research output: Contribution to journalArticle

RNA
Small Interfering RNA
Genes
Gene Silencing
Nucleotides
37 Citations (Scopus)

Piwi Modulates Chromatin Accessibility by Regulating Multiple Factors Including Histone H1 to Repress Transposons

Iwasaki, Y., Murano, K., Ishizu, H., Shibuya, A., Iyoda, Y., Siomi, M. C., Siomi, H. & Saito, K., 2015 Dec 16, (Accepted/In press) In : Molecular Cell.

Research output: Contribution to journalArticle

Histones
Chromatin
Heterochromatin
Small Interfering RNA
DNA Transposable Elements
42 Citations (Scopus)

Respective functions of two distinct siwi complexes assembled during PIWI-interacting RNA biogenesis in bombyx germ cells

Nishida, K. M., Iwasaki, Y., Murota, Y., Nagao, A., Mannen, T., Kato, Y., Siomi, H. & Siomi, M. C., 2015 Jan 13, In : Cell Reports. 10, 2, p. 193-203 11 p.

Research output: Contribution to journalArticle

Bombyx
Germ Cells
Cells
RNA
RNA Cleavage
1 Citation (Scopus)

Small RNAs: Artificial piRNAs for transcriptional silencing

Hirano, T. & Siomi, H., 2015, In : Current Biology. 25, 7, p. R280-R283

Research output: Contribution to journalArticle

DNA methylation
DNA Methylation
Transgenes
Germ Cells
Small Interfering RNA
30 Citations (Scopus)

Somatic Primary piRNA Biogenesis Driven by cis-Acting RNA Elements and trans-Acting Yb

Ishizu, H., Iwasaki, Y., Hirakata, S., Ozaki, H., Iwasaki, W., Siomi, H. & Siomi, M. C., 2015 Jul 21, In : Cell Reports. 12, 3, p. 429-440 12 p.

Research output: Contribution to journalArticle

Small Interfering RNA
RNA
3' Untranslated Regions
Messenger RNA
Gene Silencing
1 Citation (Scopus)

Sphere-formation culture of testicular germ cells in the common marmoset, a small New World monkey

Lin, Z. Y. C., Hikabe, O., Suzuki, S., Hirano, T., Siomi, H., Sasaki, E., Imamura, M. & Okano, H., 2015 Nov 3, (Accepted/In press) In : Primates.

Research output: Contribution to journalArticle

Cebidae
Callithrix jacchus
spermatogonia
germ cells
Primates
3 Citations (Scopus)

Tudor-domain containing proteins act to make the piRNA pathways more robust in Drosophila

Sato, K., Iwasaki, Y. W., Siomi, H. & Siomi, M. C., 2015 Jan 1, In : Fly. 9, 2, p. 86-90 5 p.

Research output: Contribution to journalArticle

Drosophila
RNA
proteins
transposons
trailers
2014
6 Citations (Scopus)

DiRNA-Ago2-RAD51 complexes at double-strand break sites

Yamanaka, S. & Siomi, H., 2014, In : Cell Research. 24, 5, p. 511-512 2 p.

Research output: Contribution to journalArticle

Rad51 Recombinase
Argonaute Proteins
Double-Stranded DNA Breaks
Homologous Recombination
DNA Repair
16 Citations (Scopus)

Endothelin receptor B2 ( EDNRB2) is responsible for the tyrosinase-independent recessive white (mow) and mottled (mo) plumage phenotypes in the chicken

Kinoshita, K., Akiyama, T., Mizutani, M., Shinomiya, A., Ishikawa, A., Younis, H. H., Tsudzuki, M., Namikawa, T. & Matsuda, Y., 2014 Jan 23, In : PLoS One. 9, 1, e86361.

Research output: Contribution to journalArticle

Endothelin Receptors
endothelins
Monophenol Monooxygenase
plumage
Chickens
1 Citation (Scopus)

It's time to exploit your favorite quirky organism with new technologies

Siomi, H., 2014, In : EMBO Reports. 15, 6, p. 620-621 2 p.

Research output: Contribution to journalArticle

Technology
Gene expression
Gene Expression
7 Citations (Scopus)

Lack of association between winter coat colour and genetic population structure in the Japanese hare, Lepus brachyurus (Lagomorpha: Leporidae)

Nunome, M., Kinoshita, G., Tomozawa, M., Torii, H., Matsuki, R., Yamada, F., Matsuda, Y. & Suzuki, H., 2014, In : Biological Journal of the Linnean Society. 111, 4, p. 761-776 16 p.

Research output: Contribution to journalArticle

Leporidae
Lagomorpha
Lepus
hares
population structure
3 Citations (Scopus)

miRNA regulatory ecosystem in early development

Iwasaki, Y. & Siomi, H., 2014 Dec 4, In : Molecular Cell. 56, 5, p. 615-616 2 p.

Research output: Contribution to journalArticle

MicroRNAs
Ecosystem
Mothers
Embryonic Development
Gene Expression
30 Citations (Scopus)

PiRNA clusters and open chromatin structure

Yamanaka, S., Siomi, M. C. & Siomi, H., 2014 Aug 1, In : Mobile DNA. 5, 1, 22.

Research output: Contribution to journalArticle

DNA Transposable Elements
Small Interfering RNA
Chromatin
Genome Components
Genome

RNAi and overexpression of genes in ovarian somatic cells

Saito, K., 2014, Methods in Molecular Biology. Vol. 1093. p. 25-33 9 p. (Methods in Molecular Biology; vol. 1093).

Research output: Chapter in Book/Report/Conference proceedingChapter

RNA Interference
Germ Cells
Drosophila
Genes
Cell Line
5 Citations (Scopus)

RNAi and overexpression of genes in ovarian somatic cells

Saito, K., 2014, Methods in Molecular Biology. Humana Press Inc., Vol. 1093. p. 25-33 9 p. (Methods in Molecular Biology; vol. 1093).

Research output: Chapter in Book/Report/Conference proceedingChapter

RNA Interference
Germ Cells
Drosophila
Genes
Cell Line
46 Citations (Scopus)

Small RNA profiling and characterization of piRNA clusters in the adult testes of the common marmoset, a model primate

Hirano, T., Iwasaki, Y., Lin, Z. Y. C., Imamura, M., Seki, N. M., Sasaki, E., Saito, K., Okano, H., Siomi, M. C. & Siomi, H., 2014, In : RNA. 20, 8, p. 1223-1237 15 p.

Research output: Contribution to journalArticle

Callithrix
Primates
Small Interfering RNA
Testis
RNA
47 Citations (Scopus)

Tissue-specific and ubiquitous gene knockouts by TALEN electroporation provide new approaches to investigating gene function in Ciona

Treen, N., Yoshida, K., Sakuma, T., Sasaki, H., Kawai, N., Yamamoto, T. & Sasakura, Y., 2014 Jan 15, In : Development (Cambridge). 141, 2, p. 481-487 7 p.

Research output: Contribution to journalArticle

Gene Knockout Techniques
Electroporation
Urochordata
Genes
Ciona intestinalis
39 Citations (Scopus)

Yb integrates piRNA intermediates and processing factors into perinuclear bodies to enhance piRISC assembly

Murota, Y., Ishizu, H., Nakagawa, S., Iwasaki, Y., Shibata, S., Kamatani, M. K., Saito, K., Okano, H., Siomi, H. & Siomi, M. C., 2014 Jul 10, In : Cell Reports. 8, 1, p. 103-113 11 p.

Research output: Contribution to journalArticle

Small Interfering RNA
Processing
Genes
RNA
RNA Interference