The Sakaguchi Laboratory

Research Output

2015

Maternal TET3 is dispensable for embryonic development but is required for neonatal growth

Tsukada, Y. I., Akiyama, T. & Nakayama, K. I., 2015 Oct 28, In : Scientific reports. 5, 15876.

Research output: Contribution to journalArticle

Open Access
21 Citations (Scopus)

Mini-Gut Organoids: Reconstitution of the Stem Cell Niche

Date, S. & Sato, T., 2015 Nov 13, In : Annual Review of Cell and Developmental Biology. 31, p. 269-289 21 p.

Research output: Contribution to journalArticle

76 Citations (Scopus)

Modeling colorectal cancer using CRISPR-Cas9-mediated engineering of human intestinal organoids

Matano, M., Date, S., Shimokawa, M., Takano, A., Fujii, M., Ohta, Y., Watanabe, T., Kanai, T. & Sato, T., 2015 Mar, In : Nature medicine. 21, 3, p. 256-262 7 p.

Research output: Contribution to journalArticle

430 Citations (Scopus)

Recognition of and recent issues in hereditary diffuse gastric cancer

Sugimoto, S., Komatsu, H., Morohoshi, Y. & Kanai, T., 2015 Aug 11, In : Journal of gastroenterology. 50, 8, p. 831-843 13 p.

Research output: Contribution to journalReview article

8 Citations (Scopus)

SnapShot: Growing Organoids from Stem Cells

Sato, T. & Clevers, H., 2015 Jun 20, In : Cell. 161, 7, p. 1700-1700.e1

Research output: Contribution to journalComment/debate

64 Citations (Scopus)

Suppressing TGFβ signaling in regenerating epithelia in an inflammatory microenvironment is sufficient to cause invasive intestinal cancer

Oshima, H., Nakayama, M., Han, T. S., Naoi, K., Ju, X., Maeda, Y., Robine, S., Tsuchiya, K., Sato, T., Sato, H., Taketo, M. M. & Oshima, M., 2015 Feb 15, In : Cancer Research. 75, 4, p. 766-776 11 p.

Research output: Contribution to journalArticle

45 Citations (Scopus)

Transient bursts of Zscan4 expression are accompanied by the rapid derepression of heterochromatin in mouse embryonic stem cells

Akiyama, T., Xin, L., Oda, M., Sharov, A. A., Amano, M., Piao, Y., Cadet, J. S., Dudekula, D. B., Qian, Y., Wang, W., Ko, S. B. H. & Ko, M. S. H., 2015 May 6, In : DNA Research. 22, 5, p. 307-318 12 p.

Research output: Contribution to journalArticle

37 Citations (Scopus)
2016

A Colorectal Tumor Organoid Library Demonstrates Progressive Loss of Niche Factor Requirements during Tumorigenesis

Fujii, M., Shimokawa, M., Date, S., Takano, A., Matano, M., Nanki, K., Ohta, Y., Toshimitsu, K., Nakazato, Y., Kawasaki, K., Uraoka, T., Watanabe, T., Kanai, T. & Sato, T., 2016 Jun 2, In : Cell Stem Cell. 18, 6, p. 827-838 12 p.

Research output: Contribution to journalArticle

212 Citations (Scopus)

Active and water-soluble form of lipidated wnt protein is maintained by a serum glycoprotein afamin/α-albumin

Mihara, E., Hirai, H., Yamamoto, H., Tamura-Kawakami, K., Matano, M., Kikuchi, A., Sato, T. & Takagi, J., 2016 Feb 23, In : eLife. 5, FEBRUARY2016, e11621.

Research output: Contribution to journalArticle

58 Citations (Scopus)

Clinical Efficacy and Safety of Oral Qing-Dai in Patients with Ulcerative Colitis: A Single-Center Open-Label Prospective Study

Sugimoto, S., Naganuma, M., Kiyohara, H., Arai, M., Ono, K., Mori, K., Saigusa, K., Nanki, K., Takeshita, K., Takeshita, T., Mutaguchi, M., Mizuno, S., Bessho, R., Nakazato, Y., Hisamatsu, T., Inoue, N., Ogata, H., Iwao, Y. & Kanai, T., 2016 Mar 10, (Accepted/In press) In : Digestion. p. 193-201 9 p.

Research output: Contribution to journalArticle

38 Citations (Scopus)

Dephosphorylated parafibromin is a transcriptional coactivator of the Wnt/Hedgehog/Notch pathways

Kikuchi, I., Takahashi-Kanemitsu, A., Sakiyama, N., Tang, C., Tang, P. J., Noda, S., Nakao, K., Kassai, H., Sato, T., Aiba, A. & Hatakeyama, M., 2016 Sep 21, In : Nature communications. 7, 12887.

Research output: Contribution to journalArticle

19 Citations (Scopus)

Discrepancy in the classification of water immersion from water exchange colonoscopy

Sugimoto, S. & Mizukami, T., 2016 Mar 1, In : Gastrointestinal Endoscopy. 83, 3, p. 676-677 2 p.

Research output: Contribution to journalLetter

EHBP1L1 coordinates Rab8 and Bin1 to regulate apical-directed transport in polarized epithelial cells

Nakajo, A., Yoshimura, S. I., Togawa, H., Kunii, M., Iwano, T., Izumi, A., Noguchi, Y., Watanabe, A., Goto, A., Sato, T. & Harada, A., 2016, In : Journal of Cell Biology. 212, 3, p. 297-306 10 p.

Research output: Contribution to journalArticle

18 Citations (Scopus)

Emergence of undifferentiated colonies from mouse embryonic stem cells undergoing differentiation by retinoic acid treatment

Sharova, L. V., Sharov, A. A., Piao, Y., Stagg, C. A., Amano, T., Qian, Y., Dudekula, D., Schlessinger, D. & Ko, M., 2016 Apr 29, (Accepted/In press) In : In Vitro Cellular and Developmental Biology - Animal. p. 1-9 9 p.

Research output: Contribution to journalArticle

6 Citations (Scopus)

Endoscopic Severity Predicts Long-Term Prognosis in Crohn's Disease Patients with Clinical Remission

Naganuma, M., Hisamatsu, T., Matsuoka, K., Kiyohara, H., Arai, M., Sugimoto, S., Mori, K., Nanki, K., Ohno, K., Mutaguchi, M., Mizuno, S., Bessho, R., Nakazato, Y., Hosoe, N., Inoue, N., Iwao, Y., Ogata, H. & Kanai, T., 2016 Jan 1, In : Digestion. 93, 1, p. 66-71 6 p.

Research output: Contribution to journalArticle

2 Citations (Scopus)

Gene array analysis of neural crest cells identifies transcription factors necessary for direct conversion of embryonic fibroblasts into neural crest cells

Motohashi, T., Watanabe, N., Nishioka, M., Nakatake, Y., Yulan, P., Mochizuki, H., Kawamura, Y., Ko, M. S. H., Goshima, N. & Kunisada, T., 2016 Mar 15, In : Biology Open. 5, 3, p. 311-322 12 p.

Research output: Contribution to journalArticle

5 Citations (Scopus)

Generation and gene expression profiling of 48 transcription-factor-inducible mouse embryonic stem cell lines

Yamamizu, K., Sharov, A. A., Piao, Y., Amano, M., Yu, H., Nishiyama, A., Dudekula, D. B., Schlessinger, D. & Ko, M. S. H., 2016 May 6, In : Scientific reports. 6, 25667.

Research output: Contribution to journalArticle

11 Citations (Scopus)

H1foo has a pivotal role in qualifying induced pluripotent stem cells

Kunitomi, A., Yuasa, S., Sugiyama, F., Saito, Y., Seki, T., Kusumoto, D., Kashimura, S., Takei, M., Tohyama, S., Hashimoto, H., Egashira, T., Tanimoto, Y., Mizuno, S., Tanaka, S., Okuno, H., Yamazawa, K., Watanabe, H., Oda, M., Kaneda, R., Matsuzaki, Y. & 5 others, Nagai, T., Okano, H., Yagami, K. I., Tanaka, M. & Fukuda, K., 2016 Jun 14, In : Stem cell reports. 6, 6, p. 825-833 9 p.

Research output: Contribution to journalArticle

16 Citations (Scopus)

Indole compounds may be promising medicines for ulcerative colitis

Sugimoto, S., Naganuma, M. & Kanai, T., 2016 May 9, (Accepted/In press) In : Journal of Gastroenterology. p. 1-9 9 p.

Research output: Contribution to journalArticle

49 Citations (Scopus)

Induction of specific neuron types by overexpression of single transcription factors

Teratani-Ota, Y., Yamamizu, K., Piao, Y., Sharova, L., Amano, M., Yu, H., Schlessinger, D., Ko, M. S. H. & Sharov, A. A., 2016 Oct 1, In : In Vitro Cellular and Developmental Biology - Animal. 52, 9, p. 961-973 13 p.

Research output: Contribution to journalArticle

3 Citations (Scopus)

Inhibition of DNA Methylation Suppresses Intestinal Tumor Organoids by Inducing an Anti-Viral Response

Saito, Y., Nakaoka, T., Sakai, K., Muramatsu, T., Toshimitsu, K., Kimura, M., Kanai, T., Sato, T. & Saito, H., 2016 May 4, In : Scientific reports. 6, 25311.

Research output: Contribution to journalArticle

17 Citations (Scopus)

Mule Regulates the Intestinal Stem Cell Niche via the Wnt Pathway and Targets EphB3 for Proteasomal and Lysosomal Degradation

Dominguez-Brauer, C., Hao, Z., Elia, A. J., Fortin, J. M., Nechanitzky, R., Brauer, P. M., Sheng, Y., Mana, M. D., Chio, I. I. C., Haight, J., Pollett, A., Cairns, R., Tworzyanski, L., Inoue, S., Reardon, C., Marques, A., Silvester, J., Cox, M. A., Wakeham, A., Yilmaz, O. H. & 5 others, Sabatini, D. M., van Es, J. H., Clevers, H., Sato, T. & Mak, T. W., 2016 Aug 4, In : Cell stem cell. 19, 2, p. 205-216 12 p.

Research output: Contribution to journalArticle

9 Citations (Scopus)

Osteoprotegerin regulates pancreatic β-cell homeostasis upon microbial invasion

Kuroda, Y., Maruyama, K., Fujii, H., Sugawara, I., Ko, S. B. H., Yasuda, H., Matsui, H. & Matsuo, K., 2016 Jan 11, In : PloS one. 11, 1, e0146544.

Research output: Contribution to journalArticle

8 Citations (Scopus)

p38α Activates Purine Metabolism to Initiate Hematopoietic Stem/Progenitor Cell Cycling in Response to Stress

Karigane, D., Kobayashi, H., Morikawa, T., Ootomo, Y., Sakai, M., Nagamatsu, G., Kubota, Y., Goda, N., Matsumoto, M., Nishimura, E. K., Soga, T., Otsu, K., Suematsu, M., Okamoto, S., Suda, T. & Takubo, K., 2016 Aug 4, In : Cell stem cell. 19, 2, p. 192-204 13 p.

Research output: Contribution to journalArticle

30 Citations (Scopus)

Recent trends and future directions for the medical treatment of ulcerative colitis

Naganuma, M., Mizuno, S., Nanki, K., Sugimoto, S. & Kanai, T., 2016 Dec 1, In : Clinical Journal of Gastroenterology. 9, 6, p. 329-336 8 p.

Research output: Contribution to journalReview article

18 Citations (Scopus)

SETD7 Controls Intestinal Regeneration and Tumorigenesis by Regulating Wnt/β-Catenin and Hippo/YAP Signaling

Oudhoff, M. J., Braam, M. J. S., Freeman, S. A., Wong, D., Rattray, D. G., Wang, J., Antignano, F., Snyder, K., Refaeli, I., Hughes, M. R., McNagny, K. M., Gold, M. R., Arrowsmith, C. H., Sato, T., Rossi, F. M. V., Tatlock, J. H., Owen, D. R., Brown, P. J. & Zaph, C., 2016 Apr 4, In : Developmental Cell. 37, 1, p. 47-57 11 p.

Research output: Contribution to journalArticle

42 Citations (Scopus)

The ulcerative colitis endoscopic index of severity is useful to predict medium- to long-term prognosis in ulcerative colitis patients with clinical remission

Arai, M., Naganuma, M., Sugimoto, S., Kiyohara, H., Ono, K., Mori, K., Saigusa, K., Nanki, K., Mutaguchi, M., Mizuno, S., Bessho, R., Nakazato, Y., Hosoe, N., Matsuoka, K., Inoue, N., Ogata, H., Iwao, Y. & Kanai, T., 2016, In : Journal of Crohn's and Colitis. 10, 11, p. 1303-1309 7 p.

Research output: Contribution to journalArticle

32 Citations (Scopus)

Tissue-specific mutation accumulation in human adult stem cells during life

Blokzijl, F., De Ligt, J., Jager, M., Sasselli, V., Roerink, S., Sasaki, N., Huch, M., Boymans, S., Kuijk, E., Prins, P., Nijman, I. J., Martincorena, I., Mokry, M., Wiegerinck, C. L., Middendorp, S., Sato, T., Schwank, G., Nieuwenhuis, E. E. S., Verstegen, M. M. A., Van Der Laan, L. J. W. & 8 others, De Jonge, J., Ijzermans, J. N. M., Vries, R. G., Van De Wetering, M., Stratton, M. R., Clevers, H., Cuppen, E. & Van Boxtel, R., 2016, In : Nature. 538, 7624, p. 260-264 5 p.

Research output: Contribution to journalArticle

287 Citations (Scopus)

Transient ectopic expression of the histone demethylase JMJD3 accelerates the differentiation of human pluripotent stem cells

Akiyama, T., Wakabayashi, S., Soma, A., Sato, S., Nakatake, Y., Oda, M., Murakami, M., Sakota, M., Chikazawa-Nohtomi, N., Ko, S. B. H. & Ko, M. S. H., 2016 Oct 15, In : Development (Cambridge). 143, 20, p. 3674-3685 12 p.

Research output: Contribution to journalArticle

20 Citations (Scopus)

Ulcerative colitis endoscopic index of severity is associated with long-term prognosis in ulcerative colitis patients treated with infliximab

Saigusa, K., Matsuoka, K., Sugimoto, S., Arai, M., Kiyohara, H., Takeshita, K., Mizuno, S., Mori, K., Nanki, K., Takeshita, T., Nakazato, Y., Yajima, T., Naganuma, M., Hisamatsu, T., Ogata, H., Iwao, Y. & Kanai, T., 2016 Sep 1, In : Digestive Endoscopy. 28, 6, p. 665-670 6 p.

Research output: Contribution to journalArticle

12 Citations (Scopus)

Zinc Transporter SLC39A7/ZIP7 Promotes Intestinal Epithelial Self-Renewal by Resolving ER Stress

Ohashi, W., Kimura, S., Iwanaga, T., Furusawa, Y., Irié, T., Izumi, H., Watanabe, T., Hijikata, A., Hara, T., Ohara, O., Koseki, H., Sato, T., Robine, S., Mori, H., Hattori, Y., Watarai, H., Mishima, K., Ohno, H., Hase, K. & Fukada, T., 2016 Oct, In : PLoS Genetics. 12, 10, e1006349.

Research output: Contribution to journalArticle

35 Citations (Scopus)
23 Citations (Scopus)
2017

Bicarbonate-rich fluid secretion predicted by a computational model of guinea-pig pancreatic duct epithelium

Yamaguchi, M., Steward, M. C., Smallbone, K., Sohma, Y., Yamamoto, A., Ko, S. B. H., Kondo, T. & Ishiguro, H., 2017 Mar 15, In : Journal of Physiology. 595, 6, p. 1947-1972 26 p.

Research output: Contribution to journalArticle

9 Citations (Scopus)

Cell competition with normal epithelial cells promotes apical extrusion of transformed cells through metabolic changes

Kon, S., Ishibashi, K., Katoh, H., Kitamoto, S., Shirai, T., Tanaka, S., Kajita, M., Ishikawa, S., Yamauchi, H., Yako, Y., Kamasaki, T., Matsumoto, T., Watanabe, H., Egami, R., Sasaki, A., Nishikawa, A., Kameda, I., Maruyama, T., Narumi, R., Morita, T. & 19 others, Sasaki, Y., Enoki, R., Honma, S., Imamura, H., Oshima, M., Soga, T., Miyazaki, J. I., Duchen, M. R., Nam, J. M., Onodera, Y., Yoshioka, S., Kikuta, J., Ishii, M., Imajo, M., Nishida, E., Fujioka, Y., Ohba, Y., Sato, T. & Fujita, Y., 2017 May 1, In : Nature Cell Biology. 19, 5, p. 530-541 12 p.

Research output: Contribution to journalArticle

55 Citations (Scopus)

Defining the role of Lgr5+ stem cells in colorectal cancer: From basic research to clinical applications

Fujii, M. & Sato, T., 2017 Jul 18, In : Genome Medicine. 9, 1, 66.

Research output: Contribution to journalComment/debate

2 Citations (Scopus)

Endocytoscopy can be used to assess histological healing in ulcerative colitis

Nakazato, Y., Naganuma, M., Sugimoto, S., Bessho, R., Arai, M., Kiyohara, H., Ono, K., Nanki, K., Mutaguchi, M., Mizuno, S., Kobayashi, T., Hosoe, N., Shimoda, M., Abe, T., Inoue, N., Ogata, H., Iwao, Y. & Kanai, T., 2017 Mar 13, (Accepted/In press) In : Endoscopy.

Research output: Contribution to journalArticle

13 Citations (Scopus)

Endoscopic morphologic features of ulcerative colitis–associated dysplasia classified according to the SCENIC consensus statement

Sugimoto, S., Naganuma, M., Iwao, Y., Matsuoka, K., Shimoda, M., Mikami, S., Mizuno, S., Nakazato, Y., Nanki, K., Inoue, N., Ogata, H. & Kanai, T., 2017 Mar 1, In : Gastrointestinal Endoscopy. 85, 3, p. 639-646.e2

Research output: Contribution to journalArticle

13 Citations (Scopus)

Epigenetic barrier against the propagation of fluctuating gene expression in embryonic stem cells

Saito, Y., Kunitomi, A., Seki, T., Tohyama, S., Kusumoto, D., Takei, M., Kashimura, S., Hashimoto, H., Yozu, G., Motoda, C., Shimojima, M., Egashira, T., Oda, M., Fukuda, K. & Yuasa, S., 2017 Sep, In : FEBS Letters. 591, 18, p. 2879-2889 11 p.

Research output: Contribution to journalLetter

Epigenetic Manipulation Facilitates the Generation of Skeletal Muscle Cells from Pluripotent Stem Cells

Akiyama, T., Wakabayashi, S., Soma, A., Sato, S., Nakatake, Y., Oda, M., Murakami, M., Sakota, M., Chikazawa-Nohtomi, N., Ko, S. B. H. & Ko, M. S. H., 2017, In : Stem Cells International. 2017, 7215010.

Research output: Contribution to journalReview article

2 Citations (Scopus)

Establishment of 3D intestinal organoid cultures from intestinal stem cells

Sugimoto, S. & Sato, T., 2017, Methods in Molecular Biology. Humana Press Inc., Vol. 1612. p. 97-105 9 p. (Methods in Molecular Biology; vol. 1612).

Research output: Chapter in Book/Report/Conference proceedingChapter

14 Citations (Scopus)

Expression analysis of the endogenous Zscan4 locus and its coding proteins in mouse ES cells and preimplantation embryos

Ishiguro, K. I., Nakatake, Y., Chikazawa-Nohtomi, N., Kimura, H., Akiyama, T., Oda, M., Ko, S. B. H. & Ko, M. S. H., 2017 Feb 1, In : In Vitro Cellular and Developmental Biology - Animal. 53, 2, p. 179-190 12 p.

Research output: Contribution to journalArticle

1 Citation (Scopus)

Identification of transcription factors that promote the differentiation of human pluripotent stem cells into lacrimal gland epithelium-like cells

Hirayama, M., Ko, S., Kawakita, T., Akiyama, T., Goparaju, S. K., Soma, A., Nakatake, Y., Sakota, M., Chikazawa-Nohtomi, N., Shimmura, S., Tsubota, K. & Ko, M. S. H., 2017 Dec 1, In : npj Aging and Mechanisms of Disease. 3, 1, 1.

Research output: Contribution to journalArticle

9 Citations (Scopus)

IL-22BP dictates characteristics of Peyer's patch follicleassociated epithelium for antigen uptake

Jinnohara, T., Kanaya, T., Hase, K., Sakakibara, S., Kato, T., Tachibana, N., Sasaki, T., Hashimoto, Y., Sato, T., Watarai, H., Kunisawa, J., Shibata, N., Williams, I. R., Kiyono, H. & Ohno, H., 2017 Jun 1, In : Journal of Experimental Medicine. 214, 6, p. 1607-1618 12 p.

Research output: Contribution to journalArticle

24 Citations (Scopus)

Intestinal Dysbiosis and Biotin Deprivation Induce Alopecia through Overgrowth of Lactobacillus murinus in Mice

Hayashi, A., Mikami, Y., Miyamoto, K., Kamada, N., Sato, T., Mizuno, S., Naganuma, M., Teratani, T., Aoki, R., Fukuda, S., Suda, W., Hattori, M., Amagai, M., Ohyama, M. & Kanai, T., 2017 Aug 15, In : Cell Reports. 20, 7, p. 1513-1524 12 p.

Research output: Contribution to journalArticle

25 Citations (Scopus)

Intestinal Epithelial Lgr5+ Stem Cell Niche and Organoids

Sasaki, N., Sato, T. & Clevers, H., 2017 Apr 14, Biology and Engineering of Stem Cell Niches. Elsevier Inc., p. 111-125 15 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Neural differentiation of human embryonic stem cells induced by the transgene-mediated overexpression of single transcription factors

Matsushita, M., Nakatake, Y., Arai, I., Ibata, K., Kouda, K., Goparaju, S. K., Murakami, M., Sakota, M., Chikazawa-Nohtomi, N., Ko, S., Kanai, T., Yuzaki, M. & Ko, M. S. H., 2017 May 31, (Accepted/In press) In : Biochemical and Biophysical Research Communications.

Research output: Contribution to journalArticle

12 Citations (Scopus)

Rapid differentiation of human pluripotent stem cells into functional neurons by mRNAs encoding transcription factors

Goparaju, S. K., Kohda, K., Ibata, K., Soma, A., Nakatake, Y., Akiyama, T., Wakabayashi, S., Matsushita, M., Sakota, M., Kimura, H., Yuzaki, M., Ko, S. B. H. & Ko, M. S. H., 2017 Feb 13, In : Scientific reports. 7, 42367.

Research output: Contribution to journalArticle

30 Citations (Scopus)

Salt suppresses IFNγ inducible chemokines through the IFNγ-JAK1-STAT1 signaling pathway in proximal tubular cells

Arai, Y., Takahashi, D., Asano, K., Tanaka, M., Oda, M., Ko, S., Ko, M., Mandai, S., Nomura, N., Rai, T., Uchida, S. & Sohara, E., 2017 Jan 1, In : Scientific Reports. 7, 46580.

Research output: Contribution to journalArticle

1 Citation (Scopus)

SCODE: An efficient regulatory network inference algorithm from single-cell RNA-Seq during differentiation

Matsumoto, H., Kiryu, H., Furusawa, C., Ko, M. S. H., Ko, S. B. H., Gouda, N., Hayashi, T. & Nikaido, I., 2017 Aug 1, In : Bioinformatics. 33, 15, p. 2314-2321 8 p.

Research output: Contribution to journalArticle

48 Citations (Scopus)

Single fecal microbiota transplantation failed to change intestinal microbiota and had limited effectiveness against ulcerative colitis in Japanese patients

Mizuno, S., Nanki, K., Matsuoka, K., Saigusa, K., Ono, K., Arai, M., Sugimoto, S., Kiyohara, H., Nakashima, M., Takeshita, K., Naganuma, M., Suda, W., Hattori, M. & Kanai, T., 2017, In : Intestinal Research. 15, 1, p. 68-74 7 p.

Research output: Contribution to journalArticle

17 Citations (Scopus)