The Sakaguchi Laboratory

Research Output

2017

Intestinal Dysbiosis and Biotin Deprivation Induce Alopecia through Overgrowth of Lactobacillus murinus in Mice

Hayashi, A., Mikami, Y., Miyamoto, K., Kamada, N., Sato, T., Mizuno, S., Naganuma, M., Teratani, T., Aoki, R., Fukuda, S., Suda, W., Hattori, M., Amagai, M., Ohyama, M. & Kanai, T., 2017 Aug 15, In : Cell Reports. 20, 7, p. 1513-1524 12 p.

Research output: Contribution to journalArticle

21 Citations (Scopus)

Intestinal Epithelial Lgr5+ Stem Cell Niche and Organoids

Sasaki, N., Sato, T. & Clevers, H., 2017 Apr 14, Biology and Engineering of Stem Cell Niches. Elsevier Inc., p. 111-125 15 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Neural differentiation of human embryonic stem cells induced by the transgene-mediated overexpression of single transcription factors

Matsushita, M., Nakatake, Y., Arai, I., Ibata, K., Kohda, K., Goparaju, S. K., Murakami, M., Sakota, M., Chikazawa-Nohtomi, N., Ko, S., Kanai, T., Yuzaki, M. & Ko, M., 2017 May 31, (Accepted/In press) In : Biochemical and Biophysical Research Communications.

Research output: Contribution to journalArticle

11 Citations (Scopus)

Rapid differentiation of human pluripotent stem cells into functional neurons by mRNAs encoding transcription factors

Goparaju, S. K., Kohda, K., Ibata, K., Soma, A., Nakatake, Y., Akiyama, T., Wakabayashi, S., Matsushita, M., Sakota, M., Kimura, H., Yuzaki, M., Ko, S. & Ko, M., 2017 Feb 13, In : Scientific Reports. 7, 42367.

Research output: Contribution to journalArticle

25 Citations (Scopus)

Salt suppresses IFNγ inducible chemokines through the IFNγ-JAK1-STAT1 signaling pathway in proximal tubular cells

Arai, Y., Takahashi, D., Asano, K., Tanaka, M., Oda, M., Ko, S., Ko, M., Mandai, S., Nomura, N., Rai, T., Uchida, S. & Sohara, E., 2017 Jan 1, In : Scientific Reports. 7, 46580.

Research output: Contribution to journalArticle

SCODE: An efficient regulatory network inference algorithm from single-cell RNA-Seq during differentiation

Matsumoto, H., Kiryu, H., Furusawa, C., Ko, M., Ko, S., Gouda, N., Hayashi, T. & Nikaido, I., 2017 Aug 1, In : Bioinformatics. 33, 15, p. 2314-2321 8 p.

Research output: Contribution to journalArticle

37 Citations (Scopus)

Single fecal microbiota transplantation failed to change intestinal microbiota and had limited effectiveness against ulcerative colitis in Japanese patients

Mizuno, S., Nanki, K., Matsuoka, K., Saigusa, K., Ono, K., Arai, M., Sugimoto, S., Kiyohara, H., Nakashima, M., Takeshita, K., Naganuma, M., Suda, W., Hattori, M. & Kanai, T., 2017, In : Intestinal Research. 15, 1, p. 68-74 7 p.

Research output: Contribution to journalArticle

16 Citations (Scopus)

The risk factor of clinical relapse in ulcerative colitis patients with low dose 5-aminosalicylic acid as maintenance therapy: A report from the IBD registry

Fukuda, T., Naganuma, M., Sugimoto, S., Nanki, K., Mizuno, S., Mutaguchi, M., Nakazato, Y., Inoue, N., Ogata, H., Iwao, Y. & Kanai, T., 2017 Nov 1, In : PLoS One. 12, 11, e0187737.

Research output: Contribution to journalArticle

7 Citations (Scopus)

Violet Light Exposure Can Be a Preventive Strategy Against Myopia Progression

Torii, H., Kurihara, T., Seko, Y., Negishi, K., Ohnuma, K., Inaba, T., Kawashima, M., Jiang, X., Kondo, S., Miyauchi, M., Miwa, Y., Katada, Y., Mori, K., Kato, K., Tsubota, K., Goto, H., Oda, M., Hatori, M. & Tsubota, K., 2017 Feb 1, In : EBioMedicine. 15, p. 210-219 10 p.

Research output: Contribution to journalArticle

43 Citations (Scopus)

Visualization and targeting of LGR5 + human colon cancer stem cells

Shimokawa, M., Ohta, Y., Nishikori, S., Matano, M., Takano, A., Fujii, M., Date, S., Sugimoto, S., Kanai, T. & Sato, T., 2017 May 11, In : Nature. 545, 7653, p. 187-192 6 p.

Research output: Contribution to journalArticle

191 Citations (Scopus)

Zscan4 is expressed specifically during late meiotic prophase in both spermatogenesis and oogenesis

Ishiguro, K. I., Monti, M., Akiyama, T., Kimura, H., Chikazawa-Nohtomi, N., Sakota, M., Sato, S., Redi, C. A., Ko, S. B. H. & Ko, M. S. H., 2017 Feb 1, In : In Vitro Cellular and Developmental Biology - Animal. 53, 2, p. 167-178 12 p.

Research output: Contribution to journalArticle

5 Citations (Scopus)
2016

A Colorectal Tumor Organoid Library Demonstrates Progressive Loss of Niche Factor Requirements during Tumorigenesis

Fujii, M., Shimokawa, M., Date, S., Takano, A., Matano, M., Nanki, K., Ohta, Y., Toshimitsu, K., Nakazato, Y., Kawasaki, K., Uraoka, T., Watanabe, T., Kanai, T. & Sato, T., 2016 Jun 2, In : Cell Stem Cell. 18, 6, p. 827-838 12 p.

Research output: Contribution to journalArticle

190 Citations (Scopus)

Active and water-soluble form of lipidated wnt protein is maintained by a serum glycoprotein afamin/α-albumin

Mihara, E., Hirai, H., Yamamoto, H., Tamura-Kawakami, K., Matano, M., Kikuchi, A., Sato, T. & Takagi, J., 2016 Feb 23, In : eLife. 5, FEBRUARY2016, e11621.

Research output: Contribution to journalArticle

52 Citations (Scopus)

Clinical Efficacy and Safety of Oral Qing-Dai in Patients with Ulcerative Colitis: A Single-Center Open-Label Prospective Study

Sugimoto, S., Naganuma, M., Kiyohara, H., Arai, M., Ono, K., Mori, K., Saigusa, K., Nanki, K., Takeshita, K., Takeshita, T., Mutaguchi, M., Mizuno, S., Bessho, R., Nakazato, Y., Hisamatsu, T., Inoue, N., Ogata, H., Iwao, Y. & Kanai, T., 2016 Mar 10, (Accepted/In press) In : Digestion. p. 193-201 9 p.

Research output: Contribution to journalArticle

33 Citations (Scopus)

Dephosphorylated parafibromin is a transcriptional coactivator of the Wnt/Hedgehog/Notch pathways

Kikuchi, I., Takahashi-Kanemitsu, A., Sakiyama, N., Tang, C., Tang, P. J., Noda, S., Nakao, K., Kassai, H., Sato, T., Aiba, A. & Hatakeyama, M., 2016 Sep 21, In : Nature Communications. 7, 12887.

Research output: Contribution to journalArticle

19 Citations (Scopus)

Discrepancy in the classification of water immersion from water exchange colonoscopy

Sugimoto, S. & Mizukami, T., 2016 Mar 1, In : Gastrointestinal Endoscopy. 83, 3, p. 676-677 2 p.

Research output: Contribution to journalLetter

EHBP1L1 coordinates Rab8 and Bin1 to regulate apical-directed transport in polarized epithelial cells

Nakajo, A., Yoshimura, S. I., Togawa, H., Kunii, M., Iwano, T., Izumi, A., Noguchi, Y., Watanabe, A., Goto, A., Sato, T. & Harada, A., 2016 Jan 1, In : Journal of Cell Biology. 212, 3, p. 297-306 10 p.

Research output: Contribution to journalArticle

15 Citations (Scopus)

Emergence of undifferentiated colonies from mouse embryonic stem cells undergoing differentiation by retinoic acid treatment

Sharova, L. V., Sharov, A. A., Piao, Y., Stagg, C. A., Amano, T., Qian, Y., Dudekula, D., Schlessinger, D. & Ko, M., 2016 Apr 29, (Accepted/In press) In : In Vitro Cellular and Developmental Biology - Animal. p. 1-9 9 p.

Research output: Contribution to journalArticle

6 Citations (Scopus)

Endoscopic Severity Predicts Long-Term Prognosis in Crohn's Disease Patients with Clinical Remission

Naganuma, M., Hisamatsu, T., Matsuoka, K., Kiyohara, H., Arai, M., Sugimoto, S., Mori, K., Nanki, K., Ohno, K., Mutaguchi, M., Mizuno, S., Bessho, R., Nakazato, Y., Hosoe, N., Inoue, N., Iwao, Y., Ogata, H. & Kanai, T., 2016 Jan 1, In : Digestion. 93, 1, p. 66-71 6 p.

Research output: Contribution to journalArticle

2 Citations (Scopus)

Gene array analysis of neural crest cells identifies transcription factors necessary for direct conversion of embryonic fibroblasts into neural crest cells

Motohashi, T., Watanabe, N., Nishioka, M., Nakatake, Y., Yulan, P., Mochizuki, H., Kawamura, Y., Ko, M., Goshima, N. & Kunisada, T., 2016 Mar 15, In : Biology Open. 5, 3, p. 311-322 12 p.

Research output: Contribution to journalArticle

5 Citations (Scopus)

Generation and gene expression profiling of 48 transcription-factor-inducible mouse embryonic stem cell lines

Yamamizu, K., Sharov, A. A., Piao, Y., Amano, M., Yu, H., Nishiyama, A., Dudekula, D. B., Schlessinger, D. & Ko, M., 2016 May 6, In : Scientific Reports. 6, 25667.

Research output: Contribution to journalArticle

11 Citations (Scopus)

H1foo has a pivotal role in qualifying induced pluripotent stem cells

Kunitomi, A., Yuasa, S., Sugiyama, F., Saito, Y., Seki, T., Kusumoto, D., Kashimura, S., Takei, M., Tohyama, S., Hashimoto, H., Egashira, T., Tanimoto, Y., Mizuno, S., Tanaka, S., Okuno, H., Yamazawa, K., Watanabe, H., Oda, M., Kaneda, R., Matsuzaki, Y. & 5 others, Nagai, T., Okano, H., Yagami, K. I., Tanaka, M. & Fukuda, K., 2016 Jun 14, In : Stem cell reports. 6, 6, p. 825-833 9 p.

Research output: Contribution to journalArticle

15 Citations (Scopus)

Indole compounds may be promising medicines for ulcerative colitis

Sugimoto, S., Naganuma, M. & Kanai, T., 2016 May 9, (Accepted/In press) In : Journal of Gastroenterology. p. 1-9 9 p.

Research output: Contribution to journalArticle

48 Citations (Scopus)

Induction of specific neuron types by overexpression of single transcription factors

Teratani-Ota, Y., Yamamizu, K., Piao, Y., Sharova, L., Amano, M., Yu, H., Schlessinger, D., Ko, M. & Sharov, A. A., 2016 Jun 1, (Accepted/In press) In : In Vitro Cellular and Developmental Biology - Animal. p. 1-13 13 p.

Research output: Contribution to journalArticle

3 Citations (Scopus)

Inhibition of DNA Methylation Suppresses Intestinal Tumor Organoids by Inducing an Anti-Viral Response

Saito, Y., Nakaoka, T., Sakai, K., Muramatsu, T., Toshimitsu, K., Kimura, M., Kanai, T., Sato, T. & Saito, H., 2016 May 4, In : Scientific Reports. 6, 25311.

Research output: Contribution to journalArticle

15 Citations (Scopus)

Mule Regulates the Intestinal Stem Cell Niche via the Wnt Pathway and Targets EphB3 for Proteasomal and Lysosomal Degradation

Dominguez-Brauer, C., Hao, Z., Elia, A. J., Fortin, J. M., Nechanitzky, R., Brauer, P. M., Sheng, Y., Mana, M. D., Chio, I. I. C., Haight, J., Pollett, A., Cairns, R., Tworzyanski, L., Inoue, S., Reardon, C., Marques, A., Silvester, J., Cox, M. A., Wakeham, A., Yilmaz, O. H. & 5 others, Sabatini, D. M., van Es, J. H., Clevers, H., Sato, T. & Mak, T. W., 2016 Aug 4, In : Cell stem cell. 19, 2, p. 205-216 12 p.

Research output: Contribution to journalArticle

9 Citations (Scopus)

Osteoprotegerin regulates pancreatic β-cell homeostasis upon microbial invasion

Kuroda, Y., Maruyama, K., Fujii, H., Sugawara, I., Ko, S., Yasuda, H., Matsui, H. & Matsuo, K., 2016 Jan 11, In : PLoS One. 11, 1, e0146544.

Research output: Contribution to journalArticle

8 Citations (Scopus)

Recent trends and future directions for the medical treatment of ulcerative colitis

Naganuma, M., Mizuno, S., Nanki, K., Sugimoto, S. & Kanai, T., 2016 Dec 1, In : Clinical Journal of Gastroenterology. 9, 6, p. 329-336 8 p.

Research output: Contribution to journalReview article

16 Citations (Scopus)

SETD7 Controls Intestinal Regeneration and Tumorigenesis by Regulating Wnt/β-Catenin and Hippo/YAP Signaling

Oudhoff, M. J., Braam, M. J. S., Freeman, S. A., Wong, D., Rattray, D. G., Wang, J., Antignano, F., Snyder, K., Refaeli, I., Hughes, M. R., McNagny, K. M., Gold, M. R., Arrowsmith, C. H., Sato, T., Rossi, F. M. V., Tatlock, J. H., Owen, D. R., Brown, P. J. & Zaph, C., 2016 Apr 4, In : Developmental Cell. 37, 1, p. 47-57 11 p.

Research output: Contribution to journalArticle

40 Citations (Scopus)

The ulcerative colitis endoscopic index of severity is useful to predict medium- to long-term prognosis in ulcerative colitis patients with clinical remission

Arai, M., Naganuma, M., Sugimoto, S., Kiyohara, H., Ono, K., Mori, K., Saigusa, K., Nanki, K., Mutaguchi, M., Mizuno, S., Bessho, R., Nakazato, Y., Hosoe, N., Matsuoka, K., Inoue, N., Ogata, H., Iwao, Y. & Kanai, T., 2016, In : Journal of Crohn's and Colitis. 10, 11, p. 1303-1309 7 p.

Research output: Contribution to journalArticle

29 Citations (Scopus)

Transient ectopic expression of the histone demethylase JMJD3 accelerates the differentiation of human pluripotent stem cells

Akiyama, T., Wakabayashi, S., Soma, A., Sato, S., Nakatake, Y., Oda, M., Murakami, M., Sakota, M., Chikazawa-Nohtomi, N., Ko, S. & Ko, M., 2016 Oct 15, In : Development (Cambridge). 143, 20, p. 3674-3685 12 p.

Research output: Contribution to journalArticle

19 Citations (Scopus)

Ulcerative colitis endoscopic index of severity is associated with long-term prognosis in ulcerative colitis patients treated with infliximab

Saigusa, K., Matsuoka, K., Sugimoto, S., Arai, M., Kiyohara, H., Takeshita, K., Mizuno, S., Mori, K., Nanki, K., Takeshita, T., Nakazato, Y., Yajima, T., Naganuma, M., Hisamatsu, T., Ogata, H., Iwao, Y. & Kanai, T., 2016 Sep 1, In : Digestive Endoscopy. 28, 6, p. 665-670 6 p.

Research output: Contribution to journalArticle

12 Citations (Scopus)

Zinc Transporter SLC39A7/ZIP7 Promotes Intestinal Epithelial Self-Renewal by Resolving ER Stress

Ohashi, W., Kimura, S., Iwanaga, T., Furusawa, Y., Irié, T., Izumi, H., Watanabe, T., Hijikata, A., Hara, T., Ohara, O., Koseki, H., Sato, T., Robine, S., Mori, H., Hattori, Y., Watarai, H., Mishima, K., Ohno, H., Hase, K. & Fukada, T., 2016 Oct 1, In : PLoS Genetics. 12, 10, e1006349.

Research output: Contribution to journalArticle

35 Citations (Scopus)
20 Citations (Scopus)
2015

An individual based computational model of intestinal crypt fission and its application to predicting unrestrictive growth of the intestinal epithelium

Pin, C., Parker, A., Gunning, A. P., Ohta, Y., Johnson, I. T., Carding, S. R. & Sato, T., 2015 Feb 1, In : Integrative Biology (United Kingdom). 7, 2, p. 213-228 16 p.

Research output: Contribution to journalArticle

14 Citations (Scopus)

Back to 2D Culture for Ground State of Intestinal Stem Cells

Shimokawa, M. & Sato, T., 2015, In : Cell Stem Cell. 17, 1, p. 5-7 3 p.

Research output: Contribution to journalReview article

5 Citations (Scopus)

Combination therapy with infliximab and thiopurine compared to infliximab monotherapy in maintaining remission of postoperative crohn's disease

Sakuraba, A., Okamoto, S., Matsuoka, K., Sato, T., Naganuma, M., Hisamatsu, T., Iwao, Y., Ogata, H., Kanai, T. & Hibi, T., 2015 May 6, In : Digestion. 91, 3, p. 233-238 6 p.

Research output: Contribution to journalArticle

7 Citations (Scopus)

Correction of Down syndrome and Edwards syndrome aneuploidies in human cell cultures

Amano, T., Jeffries, E., Amano, M., Ko, A. C., Yu, H. & Ko, M., 2015 May 6, In : DNA Research. 22, 5, p. 331-342 12 p.

Research output: Contribution to journalArticle

12 Citations (Scopus)

Determining c-Myb protein levels can isolate functional hematopoietic stem cell subtypes

Sakamoto, H., Takeda, N., Arai, F., Hosokawa, K., Garcia, P., Suda, T., Frampton, J. & Ogawa, M., 2015 Feb 1, In : Stem Cells. 33, 2, p. 479-490 12 p.

Research output: Contribution to journalArticle

6 Citations (Scopus)

Diagnostic and therapeutic applications of water-immersion colonoscopy

Sugimoto, S. & Mizukami, T., 2015 Jun 7, In : World Journal of Gastroenterology. 21, 21, p. 6451-6459 9 p.

Research output: Contribution to journalReview article

Open Access
14 Citations (Scopus)

Efficient genetic engineering of human intestinal organoids using electroporation

Fujii, M., Matano, M., Nanki, K. & Sato, T., 2015 Oct 29, In : Nature Protocols. 10, 10, p. 1474-1485 12 p.

Research output: Contribution to journalArticle

76 Citations (Scopus)

ExAtlas: An interactive online tool for meta-analysis of gene expression data

Sharov, A. A., Schlessinger, D. & Ko, M., 2015 Dec 1, In : Journal of Bioinformatics and Computational Biology. 13, 6, 1550019.

Research output: Contribution to journalArticle

28 Citations (Scopus)

Functional characteristics of L1156F-CFTR associated with alcoholic chronic pancreatitis in Japanese

Kondo, S., Fujiki, K., Ko, S. B. H., Yamamoto, A., Nakakuki, M., Ito, Y., Shcheynikov, N., Kitagawa, M., Naruse, S. & Ishiguro, H., 2015 Aug 18, In : American Journal of Physiology - Gastrointestinal and Liver Physiology. 309, 4, p. G260-G269

Research output: Contribution to journalArticle

4 Citations (Scopus)

Ink4a/arf-dependent loss of parietal cells induced by oxidative stress promotes CD44-dependent gastric tumorigenesis

Seishima, R., Wada, T., Tsuchihashi, K., Okazaki, S., Yoshikawa, M., Oshima, H., Oshima, M., Sato, T., Hasegawa, H., Kitagawa, Y., Goldenring, J. R., Saya, H. & Nagano, O., 2015 Jun 1, In : Cancer Prevention Research. 8, 6, p. 492-501 10 p.

Research output: Contribution to journalArticle

10 Citations (Scopus)

Maternal TET3 is dispensable for embryonic development but is required for neonatal growth

Tsukada, Y. I., Akiyama, T. & Nakayama, K. I., 2015 Oct 28, In : Scientific reports. 5, 15876.

Research output: Contribution to journalArticle

Open Access
20 Citations (Scopus)

Mini-Gut Organoids: Reconstitution of the Stem Cell Niche

Date, S. & Sato, T., 2015 Nov 13, In : Annual Review of Cell and Developmental Biology. 31, p. 269-289 21 p.

Research output: Contribution to journalArticle

69 Citations (Scopus)

Modeling colorectal cancer using CRISPR-Cas9-mediated engineering of human intestinal organoids

Matano, M., Date, S., Shimokawa, M., Takano, A., Fujii, M., Ohta, Y., Watanabe, T., Kanai, T. & Sato, T., 2015 Mar, In : Nature medicine. 21, 3, p. 256-262 7 p.

Research output: Contribution to journalArticle

394 Citations (Scopus)

Recognition of and recent issues in hereditary diffuse gastric cancer

Sugimoto, S., Komatsu, H., Morohoshi, Y. & Kanai, T., 2015 Jun 7, In : Journal of Gastroenterology. 50, 8, p. 831-843 13 p.

Research output: Contribution to journalArticle

6 Citations (Scopus)

SnapShot: Growing Organoids from Stem Cells

Sato, T. & Clevers, H., 2015 Jun 20, In : Cell. 161, 7, p. 1700-1700.e1

Research output: Contribution to journalComment/debate

62 Citations (Scopus)

Suppressing TGFβ signaling in regenerating epithelia in an inflammatory microenvironment is sufficient to cause invasive intestinal cancer

Oshima, H., Nakayama, M., Han, T. S., Naoi, K., Ju, X., Maeda, Y., Robine, S., Tsuchiya, K., Sato, T., Sato, H., Taketo, M. M. & Oshima, M., 2015 Feb 15, In : Cancer Research. 75, 4, p. 766-776 11 p.

Research output: Contribution to journalArticle

42 Citations (Scopus)