• 495 Citations
  • 12 h-Index
20062020

Research output per year

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Research Output

  • 495 Citations
  • 12 h-Index
  • 32 Article
  • 4 Conference contribution
  • 1 Review article
2020

Conformational equilibrium of MinE regulates the allowable concentration ranges of a protein wave for cell division

Kohyama, S., Fujiwara, K., Yoshinaga, N. & Doi, N., 2020 Jun 11, In : Nanoscale. 12, 22, p. 11960-11970 11 p.

Research output: Contribution to journalArticle

Dielectrophoretic sorting system for screening of NAD(P)-dependent oxidoreductases.

Kanai, Y., Shimokihara, S., Shitara, S., Oyobiki, R., Watanabe, T., Einaga, Y., Wakamatsu, K., Matsumoto, Y., Fujiwara, K. & Doi, N., 2020 Jan 1, 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017. Chemical and Biological Microsystems Society, p. 1383-1384 2 p. (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Microfluidic screening system based on boron-doped diamond electrodes and dielectrophoretic sorting for directed evolution of NAD(P)-dependent oxidoreductases

Goto, H., Kanai, Y., Yotsui, A., Shimokihara, S., Shitara, S., Oyobiki, R., Fujiwara, K., Watanabe, T., Einaga, Y., Matsumoto, Y., Miki, N. & Doi, N., 2020 Feb 21, In : Lab on a Chip. 20, 4, p. 852-861 10 p.

Research output: Contribution to journalArticle

2019

Cell-sized confinement controls generation and stability of a protein wave for spatiotemporal regulation in cells

Kohyama, S., Yoshinaga, N., Yanagisawa, M., Fujiwara, K. & Doi, N., 2019 Jul 1, In : eLife. 8, e44591.

Research output: Contribution to journalArticle

Open Access
2 Citations (Scopus)

Regulation of spatiotemporal patterning in artificial cells by a defined protein expression system

Yoshida, A., Kohyama, S., Fujiwara, K., Nishikawa, S. & Doi, N., 2019 Jan 1, In : Chemical Science. 10, 48, p. 11064-11072 9 p.

Research output: Contribution to journalArticle

Open Access
1 Citation (Scopus)
2018

A dual system using compartmentalized partnered replication for selection of arsenic-responsive transcriptional regulator

Aye, S. L., Fujiwara, K. & Doi, N., 2018 Nov 1, In : Journal of Biochemistry. 164, 5, p. 341-348 8 p.

Research output: Contribution to journalArticle

2 Citations (Scopus)

Artificial Cell Fermentation as a Platform for Highly Efficient Cascade Conversion

Fujiwara, K., Adachi, T. & Doi, N., 2018 Feb 16, In : ACS Synthetic Biology. 7, 2, p. 363-370 8 p.

Research output: Contribution to journalArticle

6 Citations (Scopus)

Engineering of DNA polymerase I from Thermus thermophilus using compartmentalized self-replication

Aye, S. L., Fujiwara, K., Ueki, A. & Doi, N., 2018 May 5, In : Biochemical and Biophysical Research Communications. 499, 2, p. 170-176 7 p.

Research output: Contribution to journalArticle

3 Citations (Scopus)

Improvement of microtas for directed evolution of NAD(P)-dependent oxidoreductases

Goto, H., Kanai, Y., Yotsui, A., Shimokihara, S., Shitara, S., Einaga, Y., Matsumoto, Y., Miki, N., Fujiwara, K. & Doi, N., 2018 Jan 1, 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. Chemical and Biological Microsystems Society, p. 2247-2249 3 p. (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018; vol. 4).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Increasing Elasticity through Changes in the Secondary Structure of Gelatin by Gelation in a Microsized Lipid Space

Sakai, A., Murayama, Y., Fujiwara, K., Fujisawa, T., Sasaki, S., Kidoaki, S. & Yanagisawa, M., 2018 Apr 25, In : ACS Central Science. 4, 4, p. 477-483 7 p.

Research output: Contribution to journalArticle

8 Citations (Scopus)
2017

DNA cytoskeleton for stabilizing artificial cells

Kurokawa, C., Fujiwara, K., Morita, M., Kawamata, I., Kawagishi, Y., Sakai, A., Murayama, Y., Nomura, S. I. M., Murata, S., Takinoue, M. & Yanagisawa, M., 2017 Jul 11, In : Proceedings of the National Academy of Sciences of the United States of America. 114, 28, p. 7228-7233 6 p.

Research output: Contribution to journalArticle

36 Citations (Scopus)

Human-derived fusogenic peptides for the intracellular delivery of proteins

Sudo, K., Niikura, K., Iwaki, K., Kohyama, S., Fujiwara, K. & Doi, N., 2017 Jun 10, In : Journal of Controlled Release. 255, p. 1-11 11 p.

Research output: Contribution to journalArticle

12 Citations (Scopus)

In vitro transcription-translation using bacterial genome as a template to reconstitute intracellular profile

Fujiwara, K., Sawamura, T., Niwa, T., Deyama, T., Nomura, S. I. M., Taguchi, H. & Doi, N., 2017 Nov 2, In : Nucleic Acids Research. 45, 19, p. 11449-11458 10 p.

Research output: Contribution to journalArticle

5 Citations (Scopus)

Liposomal internal viscosity affects the fate of membrane deformation induced by hypertonic treatment

Fujiwara, K. & Yanagisawa, M., 2017 Jan 1, In : Soft Matter. 13, 48, p. 9192-9198 7 p.

Research output: Contribution to journalArticle

3 Citations (Scopus)

Universal glass-forming behavior of in vitro and living cytoplasm

Nishizawa, K., Fujiwara, K., Ikenaga, M., Nakajo, N., Yanagisawa, M. & Mizuno, D., 2017 Dec 1, In : Scientific Reports. 7, 1, 15143.

Research output: Contribution to journalArticle

12 Citations (Scopus)
2016

Biochemical preparation of cell extract for cell-free protein synthesis without physical disruption

Fujiwara, K. & Doi, N., 2016 Apr 1, In : PLoS One. 11, 4, e0154614.

Research output: Contribution to journalArticle

18 Citations (Scopus)

Identification of novel in vivo obligate GroEL/ES substrates based on data from a cell-free proteomics approach

Niwa, T., Fujiwara, K. & Taguchi, H., 2016 Jan 1, In : FEBS Letters. 590, 2, p. 251-257 7 p.

Research output: Contribution to journalArticle

4 Citations (Scopus)

In vitro selection of bispecific diabody fragments using covalent bicistronic DNA display

Nakayama, M., Komiya, S., Fujiwara, K., Horisawa, K. & Doi, N., 2016 Sep 16, In : Biochemical and Biophysical Research Communications. 478, 2, p. 606-611 6 p.

Research output: Contribution to journalArticle

1 Citation (Scopus)

PURE mRNA display for in vitro selection of single-chain antibodies

Nagumo, Y., Fujiwara, K., Horisawa, K., Yanagawa, H. & Doi, N., 2016 May 1, In : Journal of Biochemistry. 159, 5, p. 519-526 8 p.

Research output: Contribution to journalArticle

8 Citations (Scopus)
2015

Development of μtas for high-throughput screening of nad(p)-dependent oxidoreductases

Shimokihara, S., Shitara, S., Oyobiki, R., Watanabe, T., Einaga, Y., Matsumoto, Y., Fujiwara, K., Horisawa, K. & Doi, N., 2015, MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, p. 1302-1304 3 p.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Droplet-Shooting and Size-Filtration (DSSF) Method for Synthesis of Cell-Sized Liposomes with Controlled Lipid Compositions

Morita, M., Onoe, H., Yanagisawa, M., Ito, H., Ichikawa, M., Fujiwara, K., Saito, H. & Takinoue, M., 2015 Sep 1, In : ChemBioChem. 16, 14, p. 2029-2035 7 p.

Research output: Contribution to journalArticle

16 Citations (Scopus)

Generation of monodisperse cell-sized microdroplets using a centrifuge-based axisymmetric co-flowing microfluidic device

Yamashita, H., Morita, M., Sugiura, H., Fujiwara, K., Onoe, H. & Takinoue, M., 2015 Apr 1, In : Journal of Bioscience and Bioengineering. 119, 4, p. 492-495 4 p.

Research output: Contribution to journalArticle

12 Citations (Scopus)

Peptide sequences converting polyglutamine into a prion in yeast

Odani, W., Urata, K., Okuda, M., Okuma, S., Koyama, H., Pack, C. G., Fujiwara, K., Nojima, T., Kinjo, M., Kawai-Noma, S. & Taguchi, H., 2015 Feb, In : FEBS Journal. 282, 3, p. 477-490 14 p.

Research output: Contribution to journalArticle

2014

Conversion of a chaperonin GroEL-independent protein into an obligate substrate

Ishimoto, T., Fujiwara, K., Niwa, T. & Taguchi, H., 2014 Nov 14, In : Journal of Biological Chemistry. 289, 46, p. 32073-32080 8 p.

Research output: Contribution to journalArticle

8 Citations (Scopus)
17 Citations (Scopus)

Introducing micrometer-sized artificial objects into live cells: A method for cell-giant unilamellar vesicle electrofusion

Saito, A. C., Ogura, T., Fujiwara, K., Murata, S. & Nomura, S. I. M., 2014 Sep 17, In : PloS one. 9, 9, e106853.

Research output: Contribution to journalReview article

15 Citations (Scopus)

Multiple patterns of polymer gels in microspheres due to the interplay among phase separation, wetting, and gelation

Yanagisawa, M., Nigorikawa, S., Sakaue, T., Fujiwara, K. & Tokita, M., 2014 Nov 11, In : Proceedings of the National Academy of Sciences of the United States of America. 111, 45, p. 15894-15899 6 p.

Research output: Contribution to journalArticle

15 Citations (Scopus)

The rapid synthesis of cell-sized liposomes by centrifuge-based microfluidic device

Morita, M., Onoe, H., Yanagisawa, M., Fujiwara, K., Saito, H. & Takinoue, M., 2014, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, p. 336-338 3 p.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2013

A bacterial salt sensor created by multiplying phenotypes of GroE-depleted Escherichia coli

Fujiwara, K., Aoi, K. B. & Nomura, S. I. M., 2013 Nov 7, In : Analytical Methods. 5, 21, p. 5918-5922 5 p.

Research output: Contribution to journalArticle

1 Citation (Scopus)

Condensation of an Additive-Free Cell Extract to Mimic the Conditions of Live Cells

Fujiwara, K. & Nomura, S. I. M., 2013 Jan 17, In : PloS one. 8, 1, e54155.

Research output: Contribution to journalArticle

21 Citations (Scopus)

Cooperative working of bacterial chromosome replication proteins generated by a reconstituted protein expression system

Fujiwara, K., Katayama, T. & Nomura, S. I. M., 2013 Aug 1, In : Nucleic acids research. 41, 14, p. 7176-7183 8 p.

Research output: Contribution to journalArticle

32 Citations (Scopus)

Physicochemical analysis from real-time imaging of liposome tubulation reveals the characteristics of individual f-bar domain proteins

Tanaka-Takiguchi, Y., Itoh, T., Tsujita, K., Yamada, S., Yanagisawa, M., Fujiwara, K., Yamamoto, A., Ichikawa, M. & Takiguchi, K., 2013 Jan 8, In : Langmuir. 29, 1, p. 328-336 9 p.

Research output: Contribution to journalArticle

26 Citations (Scopus)
2012

Cell-Sized confinement in microspheres accelerates the reaction of gene expression

Kato, A., Yanagisawa, M., Sato, Y. T., Fujiwara, K. & Yoshikawa, K., 2012 Apr 19, In : Scientific reports. 2, 283.

Research output: Contribution to journalArticle

50 Citations (Scopus)

Mechanism of methionine synthase overexpression in chaperonin-depleted Escherichia coli

Fujiwara, K. & Taguchi, H., 2012 Apr 1, In : Microbiology. 158, 4, p. 917-924 8 p.

Research output: Contribution to journalArticle

7 Citations (Scopus)
2010

A systematic survey of in vivo obligate chaperonin-dependent substrates

Fujiwara, K., Ishihama, Y., Nakahigashi, K., Soga, T. & Taguchi, H., 2010 May 1, In : EMBO Journal. 29, 9, p. 1552-1564 13 p.

Research output: Contribution to journalArticle

115 Citations (Scopus)
2007

Filamentous morphology in GroE-depleted Escherichia coli induced by impaired folding of FtsE

Fujiwara, K. & Taguchi, H., 2007 Aug 1, In : Journal of Bacteriology. 189, 16, p. 5860-5866 7 p.

Research output: Contribution to journalArticle

32 Citations (Scopus)
2006

Involvement of the arginine repressor in lysine biosynthesis of Thermus thermophilus

Fujiwara, K., Tsubouchi, T., Kuzuyama, T. & Nishiyama, M., 2006 Dec 1, In : Microbiology. 152, 12, p. 3585-3594 10 p.

Research output: Contribution to journalArticle

5 Citations (Scopus)