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Research Output 2006 2019

  • 426 Citations
  • 12 h-Index
  • 30 Article
  • 2 Conference contribution
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Article
2019

Cell-sized confinement controls generation and stability of a protein wave for spatiotemporal regulation in cells

Kohyama, S., Yoshinaga, N., Yanagisawa, M., Fujiwara, K. & Doi, N., 2019 Jul 1, In : eLife. 8, e44591.

Research output: Contribution to journalArticle

Open Access
Protein Stability
Confined Spaces
Proteins
Cell Division
Membrane Proteins
2018
1 Citation (Scopus)

A dual system using compartmentalized partnered replication for selection of arsenic-responsive transcriptional regulator

Aye, S. L., Fujiwara, K. & Doi, N., 2018 Nov 1, In : Journal of Biochemistry. 164, 5, p. 341-348 8 p.

Research output: Contribution to journalArticle

Arsenic
Synthetic Biology
Genetic Engineering
Networks (circuits)
Biosensing Techniques
4 Citations (Scopus)

Artificial Cell Fermentation as a Platform for Highly Efficient Cascade Conversion

Fujiwara, K., Adachi, T. & Doi, N., 2018 Feb 16, In : ACS Synthetic Biology. 7, 2, p. 363-370 8 p.

Research output: Contribution to journalArticle

Artificial Cells
Fermentation
Enzymes
Stereoselectivity
Liposomes
2 Citations (Scopus)

Engineering of DNA polymerase I from Thermus thermophilus using compartmentalized self-replication

Aye, S. L., Fujiwara, K., Ueki, A. & Doi, N., 2018 May 5, In : Biochemical and Biophysical Research Communications. 499, 2, p. 170-176 7 p.

Research output: Contribution to journalArticle

Thermus thermophilus
DNA Polymerase I
spleen exonuclease
Emulsions
Taq Polymerase
5 Citations (Scopus)

Increasing Elasticity through Changes in the Secondary Structure of Gelatin by Gelation in a Microsized Lipid Space

Sakai, A., Murayama, Y., Fujiwara, K., Fujisawa, T., Sasaki, S., Kidoaki, S. & Yanagisawa, M., 2018 Apr 25, In : ACS Central Science. 4, 4, p. 477-483 7 p.

Research output: Contribution to journalArticle

Gelation
Gelatin
Lipids
Elasticity
Gels
2017
24 Citations (Scopus)

DNA cytoskeleton for stabilizing artificial cells

Kurokawa, C., Fujiwara, K., Morita, M., Kawamata, I., Kawagishi, Y., Sakai, A., Murayama, Y., Nomura, S. I. M., Murata, S., Takinoue, M. & Yanagisawa, M., 2017 Jul 11, In : Proceedings of the National Academy of Sciences of the United States of America. 114, 28, p. 7228-7233 6 p.

Research output: Contribution to journalArticle

Artificial Cells
Cytoskeleton
DNA
Nanostructures
Gels
11 Citations (Scopus)

Human-derived fusogenic peptides for the intracellular delivery of proteins

Sudo, K., Niikura, K., Iwaki, K., Kohyama, S., Fujiwara, K. & Doi, N., 2017 Jun 10, In : Journal of Controlled Release. 255, p. 1-11 11 p.

Research output: Contribution to journalArticle

Peptides
Proteins
Germ Cells
Galactosidases
Cell-Penetrating Peptides
4 Citations (Scopus)

In vitro transcription-translation using bacterial genome as a template to reconstitute intracellular profile

Fujiwara, K., Sawamura, T., Niwa, T., Deyama, T., Nomura, S. I. M., Taguchi, H. & Doi, N., 2017 Nov 2, In : Nucleic Acids Research. 45, 19, p. 11449-11458 10 p.

Research output: Contribution to journalArticle

Bacterial Genomes
Genome
RNA
Cell Extracts
In Vitro Techniques
1 Citation (Scopus)

Liposomal internal viscosity affects the fate of membrane deformation induced by hypertonic treatment

Fujiwara, K. & Yanagisawa, M., 2017 Jan 1, In : Soft Matter. 13, 48, p. 9192-9198 7 p.

Research output: Contribution to journalArticle

Viscosity
viscosity
membranes
Membranes
Membrane Lipids
5 Citations (Scopus)

Universal glass-forming behavior of in vitro and living cytoplasm

Nishizawa, K., Fujiwara, K., Ikenaga, M., Nakajo, N., Yanagisawa, M. & Mizuno, D., 2017 Dec 1, In : Scientific Reports. 7, 1, 15143.

Research output: Contribution to journalArticle

cytoplasm
glass
crowding
metabolism
macromolecules
2016
16 Citations (Scopus)

Biochemical preparation of cell extract for cell-free protein synthesis without physical disruption

Fujiwara, K. & Doi, N., 2016 Apr 1, In : PLoS One. 11, 4, e0154614.

Research output: Contribution to journalArticle

Cell Extracts
extracts
Proteins
cells
Machinery
3 Citations (Scopus)

Identification of novel in vivo obligate GroEL/ES substrates based on data from a cell-free proteomics approach

Niwa, T., Fujiwara, K. & Taguchi, H., 2016 Jan 1, In : FEBS Letters. 590, 2, p. 251-257 7 p.

Research output: Contribution to journalArticle

Proteomics
Chaperonins
Protein Folding
Substrates
Mass Spectrometry
1 Citation (Scopus)

In vitro selection of bispecific diabody fragments using covalent bicistronic DNA display

Nakayama, M., Komiya, S., Fujiwara, K., Horisawa, K. & Doi, N., 2016 Sep 16, In : Biochemical and Biophysical Research Communications. 478, 2, p. 606-611 6 p.

Research output: Contribution to journalArticle

Bispecific Antibodies
Immunoglobulin Fragments
Display devices
DNA
Libraries
6 Citations (Scopus)

PURE mRNA display for in vitro selection of single-chain antibodies

Nagumo, Y., Fujiwara, K., Horisawa, K., Yanagawa, H. & Doi, N., 2016 May 1, In : Journal of Biochemistry. 159, 5, p. 519-526 8 p.

Research output: Contribution to journalArticle

Single-Chain Antibodies
Display devices
Messenger RNA
Proteins
In Vitro Techniques
2015
13 Citations (Scopus)

Droplet-Shooting and Size-Filtration (DSSF) Method for Synthesis of Cell-Sized Liposomes with Controlled Lipid Compositions

Morita, M., Onoe, H., Yanagisawa, M., Ito, H., Ichikawa, M., Fujiwara, K., Saito, H. & Takinoue, M., 2015 Sep 1, In : ChemBioChem. 16, 14, p. 2029-2035 7 p.

Research output: Contribution to journalArticle

Liposomes
Lipids
Chemical analysis
Microfluidics
Artificial Cells
11 Citations (Scopus)

Generation of monodisperse cell-sized microdroplets using a centrifuge-based axisymmetric co-flowing microfluidic device

Yamashita, H., Morita, M., Sugiura, H., Fujiwara, K., Onoe, H. & Takinoue, M., 2015 Apr 1, In : Journal of Bioscience and Bioengineering. 119, 4, p. 492-495 4 p.

Research output: Contribution to journalArticle

Lab-On-A-Chip Devices
Centrifuges
Microfluidics
Microtechnology
Microfabrication

Peptide sequences converting polyglutamine into a prion in yeast

Odani, W., Urata, K., Okuda, M., Okuma, S., Koyama, H., Pack, C. G., Fujiwara, K., Nojima, T., Kinjo, M., Kawai-Noma, S. & Taguchi, H., 2015, In : FEBS Journal. 282, 3, p. 477-490 14 p.

Research output: Contribution to journalArticle

Prions
Yeast
Amyloid
Yeasts
Peptides
2014
7 Citations (Scopus)

Conversion of a chaperonin GroEL-independent protein into an obligate substrate

Ishimoto, T., Fujiwara, K., Niwa, T. & Taguchi, H., 2014 Nov 14, In : Journal of Biological Chemistry. 289, 46, p. 32073-32080 8 p.

Research output: Contribution to journalArticle

Chaperonins
Chaperonin 60
Escherichia coli
Substrates
Agglomeration
16 Citations (Scopus)
Unilamellar Liposomes
Liposomes
Macromolecules
Artificial Cells
Proteins
14 Citations (Scopus)

Introducing micrometer-sized artificial objects into live cells: A method for cell-giant unilamellar vesicle electrofusion

Saito, A. C., Ogura, T., Fujiwara, K., Murata, S. & Nomura, S. I. M., 2014 Sep 17, In : PLoS One. 9, 9, e106853.

Research output: Contribution to journalArticle

electrofusion
Unilamellar Liposomes
giant cells
Giant Cells
Cells
13 Citations (Scopus)

Multiple patterns of polymer gels in microspheres due to the interplay among phase separation, wetting, and gelation

Yanagisawa, M., Nigorikawa, S., Sakaue, T., Fujiwara, K. & Tokita, M., 2014 Nov 11, In : Proceedings of the National Academy of Sciences of the United States of America. 111, 45, p. 15894-15899 6 p.

Research output: Contribution to journalArticle

Gelatin
Microspheres
Polymers
Gels
Capsules
2013
1 Citation (Scopus)

A bacterial salt sensor created by multiplying phenotypes of GroE-depleted Escherichia coli

Fujiwara, K., Aoi, K. B. & Nomura, S. I. M., 2013 Nov 7, In : Analytical Methods. 5, 21, p. 5918-5922 5 p.

Research output: Contribution to journalArticle

Escherichia coli
Salts
Sensors
Chaperonins
Proteins
20 Citations (Scopus)

Condensation of an Additive-Free Cell Extract to Mimic the Conditions of Live Cells

Fujiwara, K. & Nomura, S. I. M., 2013 Jan 17, In : PLoS One. 8, 1, e54155.

Research output: Contribution to journalArticle

Cell Extracts
Condensation
extracts
Proteins
cells
26 Citations (Scopus)

Cooperative working of bacterial chromosome replication proteins generated by a reconstituted protein expression system

Fujiwara, K., Katayama, T. & Nomura, S. I. M., 2013 Aug, In : Nucleic Acids Research. 41, 14, p. 7176-7183 8 p.

Research output: Contribution to journalArticle

Bacterial Chromosomes
DNA Replication
DNA Polymerase III
Proteins
DNA Primase
26 Citations (Scopus)

Physicochemical analysis from real-time imaging of liposome tubulation reveals the characteristics of individual f-bar domain proteins

Tanaka-Takiguchi, Y., Itoh, T., Tsujita, K., Yamada, S., Yanagisawa, M., Fujiwara, K., Yamamoto, A., Ichikawa, M. & Takiguchi, K., 2013 Jan 8, In : Langmuir. 29, 1, p. 328-336 9 p.

Research output: Contribution to journalArticle

Liposomes
homology
Lipid bilayers
proteins
Proteins
2012
44 Citations (Scopus)

Cell-Sized confinement in microspheres accelerates the reaction of gene expression

Kato, A., Yanagisawa, M., Sato, Y. T., Fujiwara, K. & Yoshikawa, K., 2012, In : Scientific Reports. 2, 283.

Research output: Contribution to journalArticle

Green Fluorescent Proteins
Microspheres
Gene Expression
Oils
Lipids
7 Citations (Scopus)

Mechanism of methionine synthase overexpression in chaperonin-depleted Escherichia coli

Fujiwara, K. & Taguchi, H., 2012 Apr, In : Microbiology. 158, 4, p. 917-924 8 p.

Research output: Contribution to journalArticle

5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase
Chaperonins
Escherichia coli
S-Adenosylmethionine
Co-Repressor Proteins
2010
109 Citations (Scopus)

A systematic survey of in vivo obligate chaperonin-dependent substrates

Fujiwara, K., Ishihama, Y., Nakahigashi, K., Soga, T. & Taguchi, H., 2010 May, In : EMBO Journal. 29, 9, p. 1552-1564 13 p.

Research output: Contribution to journalArticle

Chaperonins
Substrates
Enzymes
Proteome
Alanine
2007
31 Citations (Scopus)

Filamentous morphology in GroE-depleted Escherichia coli induced by impaired folding of FtsE

Fujiwara, K. & Taguchi, H., 2007 Aug, In : Journal of Bacteriology. 189, 16, p. 5860-5866 7 p.

Research output: Contribution to journalArticle

Cell Division
Escherichia coli
Chaperonins
Cell-Free System
Protein Folding
2006
5 Citations (Scopus)

Involvement of the arginine repressor in lysine biosynthesis of Thermus thermophilus

Fujiwara, K., Tsubouchi, T., Kuzuyama, T. & Nishiyama, M., 2006 Dec, In : Microbiology. 152, 12, p. 3585-3594 10 p.

Research output: Contribution to journalArticle

Thermus thermophilus
Lysine
Arginine
Gene Knockout Techniques
Ornithine