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Research Output 1998 2019

  • 1630 Citations
  • 19 h-Index
  • 49 Article
  • 1 Chapter
2019

Nanodiscs for Structural Biology in a Membranous Environment

Yokogawa, M., Fukuda, M. & Osawa, M., 2019 Jan 1, In : Chemical & pharmaceutical bulletin. 67, 4, p. 321-326 6 p.

Research output: Contribution to journalArticle

Open Access
Membrane Proteins
Lipid bilayers
Lipid Bilayers
Micelles
Detergents

Structural mechanism underlying G protein family-specific regulation of G protein-gated inwardly rectifying potassium channel

Kano, H., Toyama, Y., Imai, S., Iwahashi, Y., Mase, Y., Yokogawa, M., Osawa, M. & Shimada, I., 2019 Dec 1, In : Nature communications. 10, 1, 2008.

Research output: Contribution to journalArticle

Open Access
Inwardly Rectifying Potassium Channel
G-Protein-Coupled Receptors
GTP-Binding Proteins
Chemical activation
proteins
2018
4 Citations (Scopus)

Characterization of the multimeric structure of poly(A)-binding protein on a poly(A) tail

Sawazaki, R., Imai, S., Yokogawa, M., Hosoda, N., Hoshino, S. I., Mio, M., Mio, K., Shimada, I. & Osawa, M., 2018 Dec 1, In : Scientific Reports. 8, 1, 19659.

Research output: Contribution to journalArticle

Poly(A)-Binding Proteins
Carrier Proteins
Molecules
Messenger RNA
Transmission electron microscopy
3 Citations (Scopus)

Functional roles of Mg2+ binding sites in ion-dependent gating of a Mg2+ channel, MgtE, revealed by solution NMR

Maruyama, T., Imai, S., Kusakizako, T., Hattori, M., Ishitani, R., Nureki, O., Ito, K., Maturana, A. D., Shimada, I. & Osawa, M., 2018 Apr 3, In : eLife. 7, e31596.

Research output: Contribution to journalArticle

Binding Sites
Nuclear magnetic resonance
Ions
Homeostasis
Divalent Cations

Nuclear magnetic resonance approaches for characterizing protein-protein interactions

Toyama, Y., Mase, Y., Kano, H., Yokogawa, M., Osawa, M. & Shimada, I., 2018, Methods in Molecular Biology. Humana Press Inc., Vol. 1684. p. 115-128 14 p. (Methods in Molecular Biology; vol. 1684).

Research output: Chapter in Book/Report/Conference proceedingChapter

Magnetic Resonance Spectroscopy
GTP-Binding Proteins
Proteins
Inwardly Rectifying Potassium Channel
Potassium Channels
5 Citations (Scopus)
Inwardly Rectifying Potassium Channel
GTP-Binding Proteins
Nuclear magnetic resonance spectroscopy
Ethanol
Neurology
2017
15 Citations (Scopus)

ATP-dependent modulation of MgtE in Mg2+ homeostasis

Tomita, A., Zhang, M., Jin, F., Zhuang, W., Takeda, H., Maruyama, T., Osawa, M., Hashimoto, K. I., Kawasaki, H., Ito, K., Dohmae, N., Ishitani, R., Shimada, I., Yan, Z., Hattori, M. & Nureki, O., 2017 Dec 1, In : Nature Communications. 8, 1, 148.

Research output: Contribution to journalArticle

homeostasis
adenosine triphosphate
Homeostasis
Adenosine Triphosphate
Modulation
16 Citations (Scopus)

Dynamic regulation of GDP binding to G proteins revealed by magnetic field-dependent NMR relaxation analyses

Toyama, Y., Kano, H., Mase, Y., Yokogawa, M., Osawa, M. & Shimada, I., 2017 Feb 22, In : Nature Communications. 8, 14523.

Research output: Contribution to journalArticle

guanosines
diphosphates
Guanosine
Diphosphates
Magnetic Fields
2016
1 Citation (Scopus)

Disulfide mapping the voltage-sensing mechanism of a voltage-dependent potassium channel

Nozaki, T., Ozawa, S. I., Harada, H., Kimura, T., Osawa, M. & Shimada, I., 2016 Nov 17, In : Scientific Reports. 6, 37303.

Research output: Contribution to journalArticle

Potassium Channels
Disulfides
Membrane Potentials
Allosteric Regulation
Voltage-Gated Potassium Channels
19 Citations (Scopus)
Chemical Phenomena
Molecular Weight
Molecular weight
Nuclear magnetic resonance
Proteins
2015
30 Citations (Scopus)

Calmodulin and STIM proteins: Two major calcium sensors in the cytoplasm and endoplasmic reticulum in memory of Professor Koichi Yagi, Hokkaido University.

Marshall, C. B., Nishikawa, T., Osawa, M., Stathopulos, P. B. & Ikura, M., 2015 May 19, In : Biochemical and Biophysical Research Communications. 460, 1, p. 5-21 17 p.

Research output: Contribution to journalArticle

Calmodulin
Endoplasmic Reticulum
Cytoplasm
Calcium
Molecules
11 Citations (Scopus)

Structural basis for the inhibition of voltage-dependent K+ channel by gating modifier toxin

Ozawa, S. I., Kimura, T., Nozaki, T., Harada, H., Shimada, I. & Osawa, M., 2015 Sep 18, In : Scientific Reports. 5, 14226.

Research output: Contribution to journalArticle

Membrane Potentials
Action Potentials
Permeability
Fluorescence
Amino Acids
2014
4 Citations (Scopus)

Backbone resonance assignments for G protein α(i3) subunit in the GDP-bound state

Mase, Y., Yokogawa, M., Osawa, M. & Shimada, I., 2014 Oct 1, In : Biomolecular NMR Assignments. 8, 2, p. 237-241 5 p.

Research output: Contribution to journalArticle

Protein Subunits
GTP-Binding Proteins
Guanosine Triphosphate
gamma-Glutamyltransferase
Chemical shift
16 Citations (Scopus)

Cross-saturation and transferred cross-saturation experiments

Ueda, T., Takeuchi, K., Nishida, N., Stampoulis, P., Kofuku, Y., Osawa, M. & Shimada, I., 2014 Apr 29, In : Quarterly Reviews of Biophysics. 753, 1, p. 1-45 45 p.

Research output: Contribution to journalArticle

Proteins
Lipid Bilayers
Magnetic Resonance Spectroscopy
Molecular Weight
Ligands
4 Citations (Scopus)

Functional dynamics of cell surface membrane proteins

Nishida, N., Osawa, M., Takeuchi, K., Imai, S., Stampoulis, P., Kofuku, Y., Ueda, T. & Shimada, I., 2014, In : Journal of Magnetic Resonance. 241, 1, p. 86-96 11 p.

Research output: Contribution to journalArticle

Membrane Proteins
Nuclear magnetic resonance
membranes
proteins
Conformations
6 Citations (Scopus)

Structural basis for the binding of the membrane-proximal C-terminal region of chemokine receptor CCR2 with the cytosolic regulator FROUNT

Esaki, K., Yoshinaga, S., Tsuji, T., Toda, E., Terashima, Y., Saitoh, T., Kohda, D., Kohno, T., Osawa, M., Ueda, T., Shimada, I., Matsushima, K. & Terasawa, H., 2014, In : FEBS Journal. 281, 24, p. 5552-5566 15 p.

Research output: Contribution to journalArticle

Chemokine Receptors
Micelles
Membranes
G-Protein-Coupled Receptors
Nuclear magnetic resonance
2013
1 Citation (Scopus)

Backbone resonance assignments for the cytoplasmic region of the Mg 2+ transporter MgtE in the Mg2+-unbound state

Maruyama, T., Imai, S., Osawa, M., Hattori, M., Ishitani, R., Nureki, O. & Shimada, I., 2013 Apr, In : Biomolecular NMR Assignments. 7, 1, p. 93-96 4 p.

Research output: Contribution to journalArticle

Dimers
Ions
Thermus thermophilus
Enzyme Activation
Genomic Instability
2012
4 Citations (Scopus)

Backbone resonance assignments for G protein αi3 subunit in the GTP-bound state

Mase, Y., Yokogawa, M., Osawa, M. & Shimada, I., 2012, In : Biomolecular NMR Assignments. 6, 2, p. 217-220 4 p.

Research output: Contribution to journalArticle

Protein Subunits
Guanosine Triphosphate
GTP-Binding Proteins
G-Protein-Coupled Receptors
Guanine Nucleotides
12 Citations (Scopus)

Biological role of the two overlapping poly(A)-binding protein interacting motifs 2 (PAM2) of eukaryotic releasing factor eRF3 in mRNA decay

Osawa, M., Hosoda, N., Nakanishi, T., Uchida, N., Kimura, T., Imai, S., Machiyama, A., Katada, T., Hoshino, S. I. & Shimada, I., 2012 Nov, In : RNA. 18, 11, p. 1957-1967 11 p.

Research output: Contribution to journalArticle

Poly(A)-Binding Proteins
Amino Acid Motifs
RNA Stability
Messenger RNA
Calorimetry
28 Citations (Scopus)

Functional dynamics of proteins revealed by solution NMR

Osawa, M., Takeuchi, K., Ueda, T., Nishida, N. & Shimada, I., 2012 Oct, In : Current Opinion in Structural Biology. 22, 5, p. 660-669 10 p.

Research output: Contribution to journalArticle

Magnetic Resonance Spectroscopy
Relaxation Therapy
X Ray Crystallography
Proteins
Observation
36 Citations (Scopus)

Functional equilibrium of the KcsA structure revealed by NMR.

Imai, S., Osawa, M., Mita, K., Toyonaga, S., Machiyama, A., Ueda, T., Takeuchi, K., Oiki, S. & Shimada, I., 2012 Nov 16, In : Journal of Biological Chemistry. 287, 47, p. 39634-39641 8 p.

Research output: Contribution to journalArticle

Conformations
Nuclear magnetic resonance
Trifluoroethanol
Population
Membranes
6 Citations (Scopus)

Spatial distribution of cytoplasmic domains of the Mg 2+- transporter MgtE, in a solution lacking Mg 2+, revealed by paramagnetic relaxation enhancement

Imai, S., Maruyama, T., Osawa, M., Hattori, M., Ishitani, R., Nureki, O. & Shimada, I., 2012 Oct, In : Biochimica et Biophysica Acta - Proteins and Proteomics. 1824, 10, p. 1129-1135 7 p.

Research output: Contribution to journalArticle

Spatial distribution
Crystal structure
Binding Sites
Cytoplasmic Structures
Simulated annealing
27 Citations (Scopus)
Gi-Go GTP-Binding Protein alpha Subunits
Inwardly Rectifying Potassium Channel
Potassium Channels
GTP-Binding Proteins
Nuclear magnetic resonance
2011
36 Citations (Scopus)
G Protein-Coupled Inwardly-Rectifying Potassium Channels
Inwardly Rectifying Potassium Channel
Heterotrimeric GTP-Binding Proteins
Porins
Protein Subunits
9 Citations (Scopus)

Structure determination of a protein assembly by amino acid selective cross-saturation

Kanamori, E., Igarashi, S., Osawa, M., Fukunishi, Y., Shimada, I. & Nakamura, H., 2011 Jan, In : Proteins: Structure, Function and Genetics. 79, 1, p. 179-190 12 p.

Research output: Contribution to journalArticle

Amino Acids
Ubiquitin
Proteins
X ray crystallography
Structural Models
2010
1 Citation (Scopus)
Histidine
Escherichia coli
Ligands
Periplasm
ATP-Binding Cassette Transporters
56 Citations (Scopus)

NMR analyses of the interaction between CCR5 and its ligand using functional reconstitution of CCR5 in lipid bilayers

Yoshiura, C., Kofuku, Y., Ueda, T., Mase, Y., Yokogawa, M., Osawa, M., Terashima, Y., Matsushima, K. & Shimada, I., 2010 May 19, In : Journal of the American Chemical Society. 132, 19, p. 6768-6777 10 p.

Research output: Contribution to journalArticle

CCR5 Receptors
Lipid bilayers
Lipid Bilayers
Lipoproteins
Ligands
16 Citations (Scopus)

Quantitative characterization of tob interactions provides the thermodynamic basis for translation termination-coupled deadenylase regulation

Ruan, L., Osawa, M., Hosoda, N., Imai, S., Machiyama, A., Katada, T., Hoshino, S. I. & Shimada, I., 2010 Sep 3, In : Journal of Biological Chemistry. 285, 36, p. 27624-27631 8 p.

Research output: Contribution to journalArticle

Poly(A)-Binding Protein I
RNA Stability
HeLa Cells
Thermodynamics
Magnetic Resonance Spectroscopy
83 Citations (Scopus)

Structural basis underlying the dual gate properties of KcsA

Imai, S., Osawa, M., Takeuchi, K. & Shimada, I., 2010 Apr 6, In : Proceedings of the National Academy of Sciences of the United States of America. 107, 14, p. 6216-6221 6 p.

Research output: Contribution to journalArticle

Potassium Channels
Magnetic Resonance Spectroscopy
Mutation
Temperature
2009
5 Citations (Scopus)

Backbone resonance assignments for the cytoplasmic regions of G protein-activated inwardly rectifying potassium channel 1 (GIRK1)

Yokogawa, M., Muramatsu, T., Takeuchi, K., Osawa, M. & Shimada, I., 2009 Jun, In : Biomolecular NMR Assignments. 3, 1, p. 125-128 4 p.

Research output: Contribution to journalArticle

G Protein-Coupled Inwardly-Rectifying Potassium Channels
Inwardly Rectifying Potassium Channel
Heterotrimeric GTP-Binding Proteins
Protein Subunits
Eukaryotic Cells
39 Citations (Scopus)

Cross-saturation and transferred cross-saturation experiments

Shimada, I., Ueda, T., Matsumoto, M., Sakakura, M., Osawa, M., Takeuchi, K., Nishida, N. & Takahashi, H., 2009 Feb, In : Progress in Nuclear Magnetic Resonance Spectroscopy. 54, 2, p. 123-140 18 p.

Research output: Contribution to journalArticle

Extracellular Matrix
Membrane Proteins
saturation
16 Citations (Scopus)

Evidence for the direct interaction of spermine with the inwardly retifying potassium channel

Osawa, M., Yokogawa, M., Muramatsu, T., Mase, Y. & Shimada, I., 2009 Sep 18, In : Journal of Biological Chemistry. 284, 38, p. 26117-26126 10 p.

Research output: Contribution to journalArticle

Spermine
Potassium Channels
Polyamines
Nuclear magnetic resonance
Inwardly Rectifying Potassium Channel
2008
13 Citations (Scopus)

Amino acid selective cross-saturation method for identification of proximal residue pairs in a protein-protein complex

Igarashi, S., Osawa, M., Takeuchi, K., Ozawa, S. I. & Shimada, I., 2008 Sep 10, In : Journal of the American Chemical Society. 130, 36, p. 12168-12176 9 p.

Research output: Contribution to journalArticle

Amino acids
Proteins
Amino Acids
Ubiquitin
Acids
2007
118 Citations (Scopus)

Mechanism of mRNA deadenylation: Evidence for a molecular interplay between translation termination factor eRF3 and mRNA deadenylases

Funakoshi, Y., Doi, Y., Hosoda, N., Uchida, N., Osawa, M., Shimada, I., Tsujimoto, M., Suzuki, T., Katada, T. & Hoshino, S. I., 2007 Dec 1, In : Genes and Development. 21, 23, p. 3135-3148 14 p.

Research output: Contribution to journalArticle

RNA Stability
Messenger RNA
Eukaryota
Carrier Proteins
mRNA deadenylase
17 Citations (Scopus)

Nickel binding to NikA: An additional binding site reconciles spectroscopy, calorimetry and crystallography

Addy, C., Ohara, M., Kawai, F., Kidera, A., Ikeguchi, M., Fuchigami, S., Osawa, M., Shimada, I., Park, S. Y., Tame, J. R. H. & Heddle, J. G., 2007 Feb, In : Acta Crystallographica Section D: Biological Crystallography. 63, 2, p. 221-229 9 p.

Research output: Contribution to journalArticle

Calorimetry
Crystallography
Nickel
crystallography
Spectrum Analysis
83 Citations (Scopus)

Structural basis of the collagen-binding mode of discoidin domain receptor 2

Ichikawa, O., Osawa, M., Nishida, N., Goshima, N., Nomura, N. & Shimada, I., 2007 Sep 19, In : EMBO Journal. 26, 18, p. 4168-4176 9 p.

Research output: Contribution to journalArticle

Fibrillar Collagens
Collagen
Binding Sites
Cell Surface Receptors
Protein-Tyrosine Kinases
49 Citations (Scopus)

Structural insights into activation of phosphatidylinositol 4-kinase (Pik1) by yeast frequenin (Frq1)

Strahl, T., Huttner, I. G., Lusin, J. D., Osawa, M., King, D., Thorner, J. & Ames, J. B., 2007 Oct 19, In : Journal of Biological Chemistry. 282, 42, p. 30949-30959 11 p.

Research output: Contribution to journalArticle

1-Phosphatidylinositol 4-Kinase
Yeast
Phosphotransferases
Yeasts
Chemical activation
2005

Erratum: Target-induced conformational adaptation of calmodulin revealed by the crystal structure of a complex with nematode Ca2+/calmodulin- dependent kinase kinase peptide (Journal of Molecular Biology (2001) 312 (59-68) DOI: 10.1006/jmbi.2001.4822)

Kurokawa, H., Osawa, M., Kurihara, H., Katayama, N., Tokumitsu, H., Swindells, M. B., Kainosho, M. & Ikura, M., 2005 Dec 9, In : Journal of Molecular Biology. 354, 4, p. 1008 1 p.

Research output: Contribution to journalArticle

Calcium-Calmodulin-Dependent Protein Kinases
Calmodulin
Molecular Biology
Phosphotransferases
Peptides
93 Citations (Scopus)

Mg2+ and Ca2+ differentially regulate DNA binding and dimerization of DREAM

Osawa, M., Dace, A., Tong, K. I., Valiveti, A., Ikura, M. & Ames, J. B., 2005 May 6, In : Journal of Biological Chemistry. 280, 18, p. 18008-18014 7 p.

Research output: Contribution to journalArticle

Dimerization
Peptide Elongation Factor 2
EF Hand Motifs
DNA
Response Elements
2003
35 Citations (Scopus)

Molecular interactions of yeast frequenin (Frq1) with the phosphatidylinositol 4-kinase isoform, Pik1

Huttner, I. G., Strahl, T., Osawa, M., King, D. S., Ames, J. B. & Thorner, J., 2003 Feb 14, In : Journal of Biological Chemistry. 278, 7, p. 4862-4874 13 p.

Research output: Contribution to journalArticle

1-Phosphatidylinositol 4-Kinase
Molecular interactions
Yeast
Protein Isoforms
Calorimetry
2002
1 Citation (Scopus)

NMR-based approach for drug discovery

Osawa, M. & Ikura, M., 2002, In : Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme. 47, 8 Suppl, p. 948-954 7 p.

Research output: Contribution to journalArticle

Molecular Conformation
Drug Design
Drug Discovery
Protein Binding
Membrane Proteins
116 Citations (Scopus)

The role of calcium-binding proteins in the control of transcription: Structure to function

Ikura, M., Osawa, M. & Ames, J. B., 2002, In : BioEssays. 24, 7, p. 625-636 12 p.

Research output: Contribution to journalArticle

EF Hand Motifs
calcium-binding proteins
Calcium-Binding Proteins
Transcription
Carrier Proteins
2001
107 Citations (Scopus)

Calcium-regulated DNA Binding and Oligomerization of the Neuronal Calcium-sensing Protein, Calsenilin/DREAM/KChIP3

Osawa, M., Tong, K. I., Lilliehook, C., Wasco, W., Buxbaum, J. D., Cheng, H. Y. M., Penninger, J. M., Ikura, M. & Ames, J. B., 2001 Nov 2, In : Journal of Biological Chemistry. 276, 44, p. 41005-41013 9 p.

Research output: Contribution to journalArticle

Kv Channel-Interacting Proteins
Oligomerization
Calcium
DNA
Proteins
88 Citations (Scopus)

Target-induced conformational adaptation of calmodulin revealed by the crystal structure of a complex with nematode Ca2+/calmodulin-dependent kinase kinase peptide

Kurokawa, H., Osawa, M., Kurihara, H., Katayama, N., Tokumitsu, H., Swindells, M. B., Kainosho, M. & Ikura, M., 2001 Sep 7, In : Journal of Molecular Biology. 312, 1, p. 59-68 10 p.

Research output: Contribution to journalArticle

Calcium-Calmodulin-Dependent Protein Kinases
Calmodulin
Phosphotransferases
Peptides
EF Hand Motifs
2000
11 Citations (Scopus)

Mechanism of superoxide dismutase-like activity of Fe(II) and Fe(III) complexes of tetrakis-N,N,N',N'(2-pyridylmethyl)ethylenediamine

Hirano, T., Hirobe, M., Kobayashi, K., Odani, A., Yamauchi, O., Osawa, M., Satow, Y. & Nagano, T., 2000 Feb, In : Chemical and Pharmaceutical Bulletin. 48, 2, p. 223-230 8 p.

Research output: Contribution to journalArticle

Superoxide Dismutase
Superoxides
Pulse Radiolysis
Radiolysis
Rate constants
1999
214 Citations (Scopus)

A novel target recognition revealed by calmodulin in complex with Ca2+-calmodulin-dependent kinase kinase

Osawa, M., Tokumitsu, H., Swindells, M. B., Kurihara, H., Orita, M., Shibanuma, T., Furuya, T. & Ikura, M., 1999, In : Nature Structural Biology. 6, 9, p. 819-824 6 p.

Research output: Contribution to journalArticle

Calcium-Calmodulin-Dependent Protein Kinases
Calmodulin
Protein Kinases
Phosphotransferases
Peptides
42 Citations (Scopus)

Evidence for calmodulin inter-domain compaction in solution induced by W-7 binding

Osawa, M., Kuwamoto, S., Izumi, Y., Yap, K. L., Ikura, M., Shibanuma, T., Yokokura, H., Hidaka, H. & Matsushima, N., 1999 Jan 15, In : FEBS Letters. 442, 2-3, p. 173-177 5 p.

Research output: Contribution to journalArticle

Calmodulin
Compaction
X ray scattering
Distribution functions
W 7
1 Citation (Scopus)

Symmetric covalent linkage of N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7) results in novel derivatives with increased inhibitory activities against calcium/calmodulin complex

Yokokura, H., Osawa, M., Inoue, T., Umezawa, I., Naito, Y., Ikura, M. & Hidaka, H., 1999, In : Drug Design and Discovery. 16, 3, p. 203-216 14 p.

Research output: Contribution to journalArticle

Calmodulin
Calcium
Calcium-Calmodulin-Dependent Protein Kinases
Calcium-Calmodulin-Dependent Protein Kinase Type 1
W 7
1998
1 Citation (Scopus)

Calmodulin: the progress in three-dimensional structure analysis

Osawa, M., Tomomori, C. & Ikura, M., 1998, In : Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme. 43, 12 Suppl, p. 1939-1944 6 p.

Research output: Contribution to journalArticle

Protein Engineering
Trifluoperazine
Protein Conformation
Calcium-Binding Proteins
Calcium Signaling
132 Citations (Scopus)

Solution structure of calmodulin-W-7 complex: The basis of diversity in molecular recognition

Osawa, M., Swindells, M. B., Tanikawa, J., Tanaka, T., Mase, T., Furuya, T. & Ikura, M., 1998 Feb 13, In : Journal of Molecular Biology. 276, 1, p. 165-176 12 p.

Research output: Contribution to journalArticle

Calmodulin
Myosin-Light-Chain Kinase
Enzymes
Smooth Muscle Myosins
Trifluoperazine