• 1316 Citations
  • 20 h-Index
1991 …2020

Research output per year

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Research Output

2020

Conformational equilibrium of MinE regulates the allowable concentration ranges of a protein wave for cell division

Kohyama, S., Fujiwara, K., Yoshinaga, N. & Doi, N., 2020 Jun 11, In : Nanoscale. 12, 22, p. 11960-11970 11 p.

Research output: Contribution to journalArticle

Dielectrophoretic sorting system for screening of NAD(P)-dependent oxidoreductases.

Kanai, Y., Shimokihara, S., Shitara, S., Oyobiki, R., Watanabe, T., Einaga, Y., Wakamatsu, K., Matsumoto, Y., Fujiwara, K. & Doi, N., 2020 Jan 1, 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017. Chemical and Biological Microsystems Society, p. 1383-1384 2 p. (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Microfluidic screening system based on boron-doped diamond electrodes and dielectrophoretic sorting for directed evolution of NAD(P)-dependent oxidoreductases

Goto, H., Kanai, Y., Yotsui, A., Shimokihara, S., Shitara, S., Oyobiki, R., Fujiwara, K., Watanabe, T., Einaga, Y., Matsumoto, Y., Miki, N. & Doi, N., 2020 Feb 21, In : Lab on a Chip. 20, 4, p. 852-861 10 p.

Research output: Contribution to journalArticle

2019

Cell-sized confinement controls generation and stability of a protein wave for spatiotemporal regulation in cells

Kohyama, S., Yoshinaga, N., Yanagisawa, M., Fujiwara, K. & Doi, N., 2019 Jul 1, In : eLife. 8, e44591.

Research output: Contribution to journalArticle

Open Access
2 Citations (Scopus)

Regulation of spatiotemporal patterning in artificial cells by a defined protein expression system

Yoshida, A., Kohyama, S., Fujiwara, K., Nishikawa, S. & Doi, N., 2019 Jan 1, In : Chemical Science. 10, 48, p. 11064-11072 9 p.

Research output: Contribution to journalArticle

Open Access
1 Citation (Scopus)
2018

A dual system using compartmentalized partnered replication for selection of arsenic-responsive transcriptional regulator

Aye, S. L., Fujiwara, K. & Doi, N., 2018 Nov 1, In : Journal of Biochemistry. 164, 5, p. 341-348 8 p.

Research output: Contribution to journalArticle

2 Citations (Scopus)

Artificial Cell Fermentation as a Platform for Highly Efficient Cascade Conversion

Fujiwara, K., Adachi, T. & Doi, N., 2018 Feb 16, In : ACS Synthetic Biology. 7, 2, p. 363-370 8 p.

Research output: Contribution to journalArticle

6 Citations (Scopus)

Engineering of DNA polymerase I from Thermus thermophilus using compartmentalized self-replication

Aye, S. L., Fujiwara, K., Ueki, A. & Doi, N., 2018 May 5, In : Biochemical and Biophysical Research Communications. 499, 2, p. 170-176 7 p.

Research output: Contribution to journalArticle

3 Citations (Scopus)

Improvement of microtas for directed evolution of NAD(P)-dependent oxidoreductases

Goto, H., Kanai, Y., Yotsui, A., Shimokihara, S., Shitara, S., Einaga, Y., Matsumoto, Y., Miki, N., Fujiwara, K. & Doi, N., 2018 Jan 1, 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. Chemical and Biological Microsystems Society, p. 2247-2249 3 p. (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018; vol. 4).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2017

Human-derived fusogenic peptides for the intracellular delivery of proteins

Sudo, K., Niikura, K., Iwaki, K., Kohyama, S., Fujiwara, K. & Doi, N., 2017 Jun 10, In : Journal of Controlled Release. 255, p. 1-11 11 p.

Research output: Contribution to journalArticle

12 Citations (Scopus)

In vitro transcription-translation using bacterial genome as a template to reconstitute intracellular profile

Fujiwara, K., Sawamura, T., Niwa, T., Deyama, T., Nomura, S. I. M., Taguchi, H. & Doi, N., 2017 Nov 2, In : Nucleic Acids Research. 45, 19, p. 11449-11458 10 p.

Research output: Contribution to journalArticle

5 Citations (Scopus)
2016

Biochemical preparation of cell extract for cell-free protein synthesis without physical disruption

Fujiwara, K. & Doi, N., 2016 Apr 1, In : PLoS One. 11, 4, e0154614.

Research output: Contribution to journalArticle

18 Citations (Scopus)

Endosomal escape efficiency of fusogenic B18 and B55 peptides fused with anti-EGFR single chain Fv as estimated by nuclear translocation

Niikura, K., Horisawa, K. & Doi, N., 2016 Jan 1, In : Journal of Biochemistry. 159, 1, p. 123-132 10 p.

Research output: Contribution to journalArticle

3 Citations (Scopus)

In vitro selection of bispecific diabody fragments using covalent bicistronic DNA display

Nakayama, M., Komiya, S., Fujiwara, K., Horisawa, K. & Doi, N., 2016 Sep 16, In : Biochemical and Biophysical Research Communications. 478, 2, p. 606-611 6 p.

Research output: Contribution to journalArticle

1 Citation (Scopus)

PURE mRNA display for in vitro selection of single-chain antibodies

Nagumo, Y., Fujiwara, K., Horisawa, K., Yanagawa, H. & Doi, N., 2016 May 1, In : Journal of Biochemistry. 159, 5, p. 519-526 8 p.

Research output: Contribution to journalArticle

8 Citations (Scopus)
2015
9 Citations (Scopus)

Development of μtas for high-throughput screening of nad(p)-dependent oxidoreductases

Shimokihara, S., Shitara, S., Oyobiki, R., Watanabe, T., Einaga, Y., Matsumoto, Y., Fujiwara, K., Horisawa, K. & Doi, N., 2015, MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, p. 1302-1304 3 p.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)
2014

Structural basis for inhibition of the MDM2:p53 interaction by an optimized MDM2-binding peptide selected with mRNA Display

Nagata, T., Shirakawa, K., Kobayashi, N., Shiheido, H., Tabata, N., Sakuma-Yonemura, Y., Horisawa, K., Katahira, M., Doi, N. & Yanagawa, H., 2014 Oct 2, In : PloS one. 9, 10, e109163.

Research output: Contribution to journalArticle

8 Citations (Scopus)

Toward high-throughput screening of NAD(P)-dependent oxidoreductases using boron-doped diamond microelectrodes and microfluidic devices

Oyobiki, R., Kato, T., Katayama, M., Sugitani, A., Watanabe, T., Einaga, Y., Matsumoto, Y., Horisawa, K. & Doi, N., 2014 Oct 7, In : Analytical chemistry. 86, 19, p. 9570-9575 6 p.

Research output: Contribution to journalArticle

17 Citations (Scopus)
2013

Hereditary spastic paraplegia protein spartin is an FK506-binding protein identified by mRNA display

Tokunaga, M., Shiheido, H., Hayakawa, I., Utsumi, A., Takashima, H., Doi, N., Horisawa, K., Sakuma-Yonemura, Y., Tabata, N. & Yanagawa, H., 2013 Jul 25, In : Chemistry and Biology. 20, 7, p. 935-942 8 p.

Research output: Contribution to journalArticle

2 Citations (Scopus)

MIP-2A Is a Novel Target of an Anilinoquinazoline Derivative for Inhibition of Tumour Cell Proliferation

Tokunaga, M., Shiheido, H., Tabata, N., Sakuma-Yonemura, Y., Takashima, H., Horisawa, K., Doi, N. & Yanagawa, H., 2013 Sep 30, In : PloS one. 8, 9, e76774.

Research output: Contribution to journalArticle

4 Citations (Scopus)
2012

An Anilinoquinazoline Derivative Inhibits Tumor Growth through Interaction with hCAP-G2, a Subunit of Condensin II

Shiheido, H., Naito, Y., Kimura, H., Genma, H., Takashima, H., Tokunaga, M., Ono, T., Hirano, T., Du, W., Yamada, T., Doi, N., Iijima, S., Hattori, Y. & Yanagawa, H., 2012 Sep 13, In : PloS one. 7, 9, e44889.

Research output: Contribution to journalArticle

10 Citations (Scopus)

A phthalimide derivative that inhibits centrosomal clustering is effective on multiple myeloma

Shiheido, H., Terada, F., Tabata, N., Hayakawa, I., Matsumura, N., Takashima, H., Ogawa, Y., Du, W., Yamada, T., Shoji, M., Sugai, T., Doi, N., Iijima, S., Hattori, Y. & Yanagawa, H., 2012 Jun 25, In : PloS one. 7, 6, e38878.

Research output: Contribution to journalArticle

22 Citations (Scopus)

DNA display selection of peptide ligands for a full-length human G protein-coupled receptor on CHO-K1 cells

Doi, N., Yamakawa, N., Matsumoto, H., Yamamoto, Y., Nagano, T., Matsumura, N., Horisawa, K. & Yanagawa, H., 2012 Jan 10, In : PloS one. 7, 1, e30084.

Research output: Contribution to journalArticle

4 Citations (Scopus)

In vitro selection of fab fragments by mRNA display and gene-linking emulsion PCR

Sumida, T., Yanagawa, H. & Doi, N., 2012 Nov 7, In : Journal of Nucleic Acids. 2012, 371379.

Research output: Contribution to journalArticle

19 Citations (Scopus)
2011

Comparison of the frequency of functional SH3 domains with different limited sets of amino acids using mRNA display

Tanaka, J., Yanagawa, H. & Doi, N., 2011 Mar 25, In : PloS one. 6, 3, e18034.

Research output: Contribution to journalArticle

13 Citations (Scopus)

High Solubility of Random-Sequence Proteins Consisting of Five Kinds of Primitive Amino Acids

Doi, N., Kakukawa, K., Oishi, Y. & Yanagawa, H., 2011 Feb 14, Chemical Synthetic Biology. John Wiley and Sons, p. 119-137 19 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

mRNA display selection of an optimized MDM2-binding peptide that potently inhibits MDM2-p53 interaction

Shiheido, H., Takashima, H., Doi, N. & Yanagawa, H., 2011 Mar 21, In : PloS one. 6, 3, e17898.

Research output: Contribution to journalArticle

18 Citations (Scopus)
2010

Comparative characterization of random-sequence proteins consisting of 5, 12, and 20 kinds of amino acids

Tanaka, J., Doi, N., Takashima, H. & Yanagawa, H., 2010 Apr 1, In : Protein Science. 19, 4, p. 786-795 10 p.

Research output: Contribution to journalArticle

21 Citations (Scopus)

Electrochemical detection of tyrosine derivatives and protein tyrosine kinase activity using boron-doped diamond electrodes

Chiku, M., Horisawa, K., Doi, N., Yanagawa, H. & Einaga, Y., 2010 Sep 1, In : Biosensors and Bioelectronics. 26, 1, p. 235-240 6 p.

Research output: Contribution to journalArticle

27 Citations (Scopus)

mRNA display selection of a high-affinity, Bcl-XL-specific binding peptide

Matsumura, N., Tsuji, T., Sumida, T., Kokubo, M., Onimaru, M., Doi, N., Takashima, H., Miyamoto-Sato, E. & Yanagawa, H., 2010 Jul 1, In : FASEB Journal. 24, 7, p. 2201-2210 10 p.

Research output: Contribution to journalArticle

11 Citations (Scopus)
2009

Bicistronic DNA display for in vitro selection of Fab fragments

Sumida, T., Doi, N. & Yanagawa, H., 2009 Sep 29, In : Nucleic acids research. 37, 22, p. e147-e147 gkp776.

Research output: Contribution to journalArticle

18 Citations (Scopus)

In vitro selection of GTP-binding proteins by block shuffling of estrogen-receptor fragments

Tsuji, T., Onimaru, M., Doi, N., Miyamoto-Sato, E., Takashima, H. & Yanagawa, H., 2009 Dec 18, In : Biochemical and Biophysical Research Communications. 390, 3, p. 689-693 5 p.

Research output: Contribution to journalArticle

7 Citations (Scopus)

Rapid antibody selection by mRNA display on a microfluidic chip

Tabata, N., Sakuma, Y., Honda, Y., Doi, N., Takashima, H., Miyamoto-Sato, E. & Yanagawa, H., 2009, In : Nucleic acids research. 37, 8, e64.

Research output: Contribution to journalArticle

26 Citations (Scopus)
2008

Use of cDNA Tiling Arrays for Identifying Protein Interactions Selected by In Vitro Display Technologies

Horisawa, K., Doi, N. & Yanagawa, H., 2008, In : PloS one. 3, 2, e0001646.

Research output: Contribution to journalArticle

7 Citations (Scopus)
2007

High-throughput fluorescence labelling of full-length cDNA products based on a reconstituted translation system

Kawahashi, Y., Doi, N., Oishi, Y., Tsuda, C., Takashima, H., Baba, T., Mori, H., Ito, T. & Yanagawa, H., 2007 Jan, In : Journal of biochemistry. 141, 1, p. 19-24 6 p.

Research output: Contribution to journalArticle

4 Citations (Scopus)

Metabolic engineering of carotenoid biosynthesis in Escherichia coli by ordered gene assembly in Bacillus subtilis

Nishizaki, T., Tsuge, K., Itaya, M., Doi, N. & Yanagawa, H., 2007 Feb 1, In : Applied and Environmental Microbiology. 73, 4, p. 1355-1361 7 p.

Research output: Contribution to journalArticle

82 Citations (Scopus)

Photocleavable linkage between genotype and phenotype for rapid and efficient recovery of nucleic acids encoding affinity-selected proteins

Doi, N., Takashima, H., Wada, A., Oishi, Y., Nagano, T. & Yanagawa, H., 2007 Sep 15, In : Journal of Biotechnology. 131, 3, p. 231-239 9 p.

Research output: Contribution to journalArticle

14 Citations (Scopus)
2006

Affinity selection of DNA-binding protein complexes using mRNA display

Tateyama, S., Horisawa, K., Takashima, H., Miyamoto-Sato, E., Doi, N. & Yanagawa, H., 2006 Mar 20, In : Nucleic acids research. 34, 3, e27.

Research output: Contribution to journalArticle

31 Citations (Scopus)

Escherichia coli proteome chips for detecting protein-protein interactions

Oishi, Y., Yunomura, S., Kawahashi, Y., Doi, N., Takashima, H., Baba, T., Mori, H. & Yanagawa, H., 2006 Dec, In : Proteomics. 6, 24, p. 6433-6436 4 p.

Research output: Contribution to journalArticle

10 Citations (Scopus)

In vitro evolution of single-chain antibodies using mRNA display

Fukuda, I., Kojoh, K., Tabata, N., Doi, N., Takashima, H., Miyamoto-Sato, E. & Yanagawa, H., 2006 Nov, In : Nucleic acids research. 34, 19, e127.

Research output: Contribution to journalArticle

61 Citations (Scopus)

Protein - Protein interaction analysis by C-terminally specific fluorescence labeling and fluorescence cross-correlation spectroscopy

Oyama, R., Takashima, H., Yonezawa, M., Doi, N., Miyamoto-Sato, E., Kinjo, M. & Yanagawa, H., 2006 Sep 21, In : Nucleic acids research. 34, 14, e102.

Research output: Contribution to journalArticle

37 Citations (Scopus)

Recent progress and future prospects in protein display technologies as tools for proteomics

Matsumura, N., Doi, N. & Yanagawa, H., 2006 Oct 1, In : Current Proteomics. 3, 3, p. 199-215 17 p.

Research output: Contribution to journalReview article

7 Citations (Scopus)
2005

Application of quantitative real-time PCR for monitoring the process of enrichment of clones on in vitro protein selection

Horisawa, K., Doi, N., Takashima, H. & Yanagawa, H., 2005 Feb 1, In : Journal of biochemistry. 137, 2, p. 121-124 4 p.

Research output: Contribution to journalArticle

6 Citations (Scopus)

High solubility of random-sequence proteins consisting of five kinds of primitive amino acids

Doi, N., Kakukawa, K., Oishi, Y. & Yanagawa, H., 2005 Jun 1, In : Protein Engineering, Design and Selection. 18, 6, p. 279-284 6 p.

Research output: Contribution to journalArticle

53 Citations (Scopus)

Inversion of enantioselectivity of asymmetric biocatalytic decarboxylation by site-directed mutagenesis based on the reaction mechanism

Ijima, Y., Matoishi, K., Terao, Y., Doi, N., Yanagawa, H. & Ohta, H., 2005 Feb 21, In : Chemical Communications. 7, p. 877-879 3 p.

Research output: Contribution to journalArticle

52 Citations (Scopus)
2004

DNA Display of Biologically Active Proteins for In Vitro Protein Selection

Yonezawa, M., Doi, N., Higashinakagawa, T. & Yanagawa, H., 2004 Mar 1, In : Journal of biochemistry. 135, 3, p. 285-288 4 p.

Research output: Contribution to journalArticle

29 Citations (Scopus)

In vitro selection of Jun-associated proteins using mRNA display.

Horisawa, K., Tateyama, S., Ishizaka, M., Matsumura, N., Takashima, H., Miyamoto-Sato, E., Doi, N. & Yanagawa, H., 2004, In : Nucleic acids research. 32, 21, p. e169

Research output: Contribution to journalArticle

Open Access
46 Citations (Scopus)

In vitro selection of restriction endonucleases by in vitro compartmentalization.

Doi, N., Kumadaki, S., Oishi, Y., Matsumura, N. & Yanagawa, H., 2004, In : Nucleic acids research. 32, 12, p. e95

Research output: Contribution to journalArticle

Open Access
39 Citations (Scopus)
2003

DNA display for in vitro selection of diverse peptide libraries

Yonezawa, M., Doi, N., Kawahashi, Y., Higashinakagawa, T. & Yanagawa, H., 2003 Oct 1, In : Nucleic acids research. 31, 19, p. e118

Research output: Contribution to journalArticle

Open Access
75 Citations (Scopus)