α-tocopherol transfer protein is important for the normal development of placental labyrinthine trophoblasts in mice

Kou Ichi Jishage, Makoto Arita, Keiji Igarashi, Takamitsu Iwata, Miho Watanabe, Masako Ogawa, Otoya Ueda, Nobuo Kamada, Keizo Inoue, Hiroyuki Arai, Hiroshi Suzuki

Research output: Contribution to journalArticle

147 Citations (Scopus)

Abstract

α-Tocopherol transfer protein (α-TTP), a cytosolic protein that specifically binds α-tocopherol, is known as a product of the causative gene in patients with ataxia that is associated with vitamin E deficiency. Targeted disruption of the α-TTP gene revealed that α-tocopherol concentration in the circulation was regulated by α-TTP expression levels. Male α-TTP-/- mice were fertile; however, placentas of pregnant α-TTP-/- females were severely impaired with marked reduction of labyrinthine trophoblasts, and the embryos died at mid-gestation even when fertilized eggs of α-TTP+/+ mice were transferred into α-TTP-/- recipients. The use of excess α-tocopherol or a synthetic antioxidant (BO-653) dietary supplement by α-TTP-/- females prevented placental failure and allowed full-term pregnancies. In α-TTP+/+ animals, α-TTP gene expression was observed in the uterus, and its level transiently increased after implantation (4.5 days postcoitum). Our results suggest that oxidative stress in the labyrinth region of the placenta is protected by vitamin E during development and that in addition to the hepatic α-TTP, which governs plasma α-tocopherol level, the uterine α-TTP may also play an important role in supplying this vitamin.

Original languageEnglish
Pages (from-to)1669-1672
Number of pages4
JournalJournal of Biological Chemistry
Volume276
Issue number3
DOIs
Publication statusPublished - 2001 Jan 19
Externally publishedYes

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Placentation
Tocopherols
Trophoblasts
Proteins
Vitamin E
Placenta
thymidine 5'-triphosphate
Dietary supplements
Genes
Vitamin E Deficiency
Pregnancy
Oxidative stress
Zygote
Inner Ear
Ataxia
Dietary Supplements
Gene expression
Vitamins
Uterus
Animals

ASJC Scopus subject areas

  • Biochemistry

Cite this

α-tocopherol transfer protein is important for the normal development of placental labyrinthine trophoblasts in mice. / Jishage, Kou Ichi; Arita, Makoto; Igarashi, Keiji; Iwata, Takamitsu; Watanabe, Miho; Ogawa, Masako; Ueda, Otoya; Kamada, Nobuo; Inoue, Keizo; Arai, Hiroyuki; Suzuki, Hiroshi.

In: Journal of Biological Chemistry, Vol. 276, No. 3, 19.01.2001, p. 1669-1672.

Research output: Contribution to journalArticle

Jishage, KI, Arita, M, Igarashi, K, Iwata, T, Watanabe, M, Ogawa, M, Ueda, O, Kamada, N, Inoue, K, Arai, H & Suzuki, H 2001, 'α-tocopherol transfer protein is important for the normal development of placental labyrinthine trophoblasts in mice', Journal of Biological Chemistry, vol. 276, no. 3, pp. 1669-1672. https://doi.org/10.1074/jbc.C000676200
Jishage, Kou Ichi ; Arita, Makoto ; Igarashi, Keiji ; Iwata, Takamitsu ; Watanabe, Miho ; Ogawa, Masako ; Ueda, Otoya ; Kamada, Nobuo ; Inoue, Keizo ; Arai, Hiroyuki ; Suzuki, Hiroshi. / α-tocopherol transfer protein is important for the normal development of placental labyrinthine trophoblasts in mice. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 3. pp. 1669-1672.
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