Abstract
Lymphocyte recirculation through the blood flow circuit and lymphoid organs is important for the maintenance of immune defense, and is defined as lymphocyte homing. During the homing process, several adhesion molecules have been postulated to play an important role in lymphocyte recruitment from the vascular space. In the present study, we investigated the effect of a novel mAb against rat α4 integrin (MRα4-1) on the interaction of T lymphocytes with postcapillary venules (PCV) and their subsequent migration into the interstitium of Peyer's patches, using intravital video microscopy. T lymphocytes collected from intestinal lymph were labeled with a fluorochrome carboxyfluorescein diacetate succinimidyl ester and were then injected into the jugular vein of recipient rats. The microvasculature in the ileal Peyer's patches of recipient rats was observed sequentially by intravital fluorescence microscopy. In controls, lymphocytes exhibited rolling behavior which was followed by firm adhesion to the endothelium of PCV. The density of sticking lymphocytes gradually increased during the first 30 min. These initial interactions of lymphocytes with the PCV (rolling and adherence) were drastically inhibited by treatment with MRα4-1, both when MRα4-1 was preinfused into rats and when T cells were preincubated in vitro with MRα4-1 before administration, MRα4-1 also significantly inhibited the transendothelial migration of T lymphocytes, assessed by the ratio of migration to adherence. However, once T lymphocytes migrated into the interstitium, treatment with MRα4-1 did not affect the pattern of travel of these lymphocytes in the interstitium or their transport into the microlymphatics in the parafollicular area. Therefore, we conclude that α4 integrins play a critical role in the rolling and sticking of T cells and their transendothelial migration in PCV of Peyer's patches.
| Original language | English |
|---|---|
| Pages (from-to) | 287-295 |
| Number of pages | 9 |
| Journal | International Immunology |
| Volume | 8 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1996 |
Fingerprint
Keywords
- α4 integrin
- Intravital microscopy
- Lymphocyle migration
- Peyer's patches
- Postcapillary venules
- Transendothelial migration
ASJC Scopus subject areas
- Immunology
Cite this
α4 integrin plays a critical role in early stages of T lymphocyte migration in Peyer's patches of rats. / Tsuzuki, Yoshikazu; Miura, Soichiro; Suematsu, Makoto; Kurose, Iwao; Shigematsu, Takeharu; Kimura, Hiroyuki; Higuchi, Hajime; Serizawa, Hiroshi; Yagita, Hideo; Okumura, Ko; Ishii, Hiromasa.
In: International Immunology, Vol. 8, No. 3, 1996, p. 287-295.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - α4 integrin plays a critical role in early stages of T lymphocyte migration in Peyer's patches of rats
AU - Tsuzuki, Yoshikazu
AU - Miura, Soichiro
AU - Suematsu, Makoto
AU - Kurose, Iwao
AU - Shigematsu, Takeharu
AU - Kimura, Hiroyuki
AU - Higuchi, Hajime
AU - Serizawa, Hiroshi
AU - Yagita, Hideo
AU - Okumura, Ko
AU - Ishii, Hiromasa
PY - 1996
Y1 - 1996
N2 - Lymphocyte recirculation through the blood flow circuit and lymphoid organs is important for the maintenance of immune defense, and is defined as lymphocyte homing. During the homing process, several adhesion molecules have been postulated to play an important role in lymphocyte recruitment from the vascular space. In the present study, we investigated the effect of a novel mAb against rat α4 integrin (MRα4-1) on the interaction of T lymphocytes with postcapillary venules (PCV) and their subsequent migration into the interstitium of Peyer's patches, using intravital video microscopy. T lymphocytes collected from intestinal lymph were labeled with a fluorochrome carboxyfluorescein diacetate succinimidyl ester and were then injected into the jugular vein of recipient rats. The microvasculature in the ileal Peyer's patches of recipient rats was observed sequentially by intravital fluorescence microscopy. In controls, lymphocytes exhibited rolling behavior which was followed by firm adhesion to the endothelium of PCV. The density of sticking lymphocytes gradually increased during the first 30 min. These initial interactions of lymphocytes with the PCV (rolling and adherence) were drastically inhibited by treatment with MRα4-1, both when MRα4-1 was preinfused into rats and when T cells were preincubated in vitro with MRα4-1 before administration, MRα4-1 also significantly inhibited the transendothelial migration of T lymphocytes, assessed by the ratio of migration to adherence. However, once T lymphocytes migrated into the interstitium, treatment with MRα4-1 did not affect the pattern of travel of these lymphocytes in the interstitium or their transport into the microlymphatics in the parafollicular area. Therefore, we conclude that α4 integrins play a critical role in the rolling and sticking of T cells and their transendothelial migration in PCV of Peyer's patches.
AB - Lymphocyte recirculation through the blood flow circuit and lymphoid organs is important for the maintenance of immune defense, and is defined as lymphocyte homing. During the homing process, several adhesion molecules have been postulated to play an important role in lymphocyte recruitment from the vascular space. In the present study, we investigated the effect of a novel mAb against rat α4 integrin (MRα4-1) on the interaction of T lymphocytes with postcapillary venules (PCV) and their subsequent migration into the interstitium of Peyer's patches, using intravital video microscopy. T lymphocytes collected from intestinal lymph were labeled with a fluorochrome carboxyfluorescein diacetate succinimidyl ester and were then injected into the jugular vein of recipient rats. The microvasculature in the ileal Peyer's patches of recipient rats was observed sequentially by intravital fluorescence microscopy. In controls, lymphocytes exhibited rolling behavior which was followed by firm adhesion to the endothelium of PCV. The density of sticking lymphocytes gradually increased during the first 30 min. These initial interactions of lymphocytes with the PCV (rolling and adherence) were drastically inhibited by treatment with MRα4-1, both when MRα4-1 was preinfused into rats and when T cells were preincubated in vitro with MRα4-1 before administration, MRα4-1 also significantly inhibited the transendothelial migration of T lymphocytes, assessed by the ratio of migration to adherence. However, once T lymphocytes migrated into the interstitium, treatment with MRα4-1 did not affect the pattern of travel of these lymphocytes in the interstitium or their transport into the microlymphatics in the parafollicular area. Therefore, we conclude that α4 integrins play a critical role in the rolling and sticking of T cells and their transendothelial migration in PCV of Peyer's patches.
KW - α4 integrin
KW - Intravital microscopy
KW - Lymphocyle migration
KW - Peyer's patches
KW - Postcapillary venules
KW - Transendothelial migration
UR - http://www.scopus.com/inward/record.url?scp=0029670925&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029670925&partnerID=8YFLogxK
U2 - 10.1093/intimm/8.3.287
DO - 10.1093/intimm/8.3.287
M3 - Article
VL - 8
SP - 287
EP - 295
JO - International Immunology
JF - International Immunology
SN - 0953-8178
IS - 3
ER -