β-adrenergic signaling promotes posteriorization in Xenopus early development

Shoko Mori, Yuki Moriyama, Kumiko Yoshikawa, Tomoyo Furukawa, Hiroki Kuroda

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Adrenaline (also known as Epinephrine) is a hormone, which works as major regulator of various biological events such stages of vertebrate, the role of adrenaline for early embryogenesis has been as heart rate, blood vessel and air passage diameters, and metabolic shifts. Although its specific receptors are expressing at the early developmental stage those functions are poorly understood. Here, we show that loss-of-functional effects of adrenergic receptor β-2 (Adrβ2), which was known as the major receptor for adrenaline and highly expressed in embryonic stages, led posterior defects at the tadpole stage of Xenopus embryos, while embryos injected with Adrβ2 mRNA or treated with adrenaline hormone adversely lost anterior structures. This posteriorization effect by adrenaline hormone was dose-dependently increased but effectively rescued by microinjection of antisense morpholino oligomer for Adrβ2 (Adrβ2-MO). Combination of adrenaline treatments and microinjection of Adrβ2 mRNA maximized efficiency in its posteriorizing activity. Interestingly, both gain- and loss-of-functional treatment for β-adrenergic signaling could not influence anterior neural fate induced by overexpression of Chordin mRNA in presumptive ectodermal region, meaning that it worked via mesoderm. Taken together with these results, we conclude that adrenaline is a novel regulator of anteroposterior axis formation in vertebrates.

Original languageEnglish
Pages (from-to)350-358
Number of pages9
JournalDevelopment Growth and Differentiation
Volume55
Issue number3
DOIs
Publication statusPublished - 2013 Apr
Externally publishedYes

Fingerprint

Xenopus
Adrenergic Agents
Epinephrine
Adrenergic Receptors
Microinjections
Hormones
Messenger RNA
Vertebrates
Embryonic Structures
Morpholinos
Mesoderm
Embryonic Development
Larva
Blood Vessels
Heart Rate
Air
Therapeutics

Keywords

  • Adrenaline
  • Anteroposterior patterning
  • Fibroblast growth factor
  • Posteriorization
  • Wnt

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

β-adrenergic signaling promotes posteriorization in Xenopus early development. / Mori, Shoko; Moriyama, Yuki; Yoshikawa, Kumiko; Furukawa, Tomoyo; Kuroda, Hiroki.

In: Development Growth and Differentiation, Vol. 55, No. 3, 04.2013, p. 350-358.

Research output: Contribution to journalArticle

Mori, Shoko ; Moriyama, Yuki ; Yoshikawa, Kumiko ; Furukawa, Tomoyo ; Kuroda, Hiroki. / β-adrenergic signaling promotes posteriorization in Xenopus early development. In: Development Growth and Differentiation. 2013 ; Vol. 55, No. 3. pp. 350-358.
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