β-catenin activation and epithelial-mesenchymal transition in the pathogenesis of pterygium

Naoko Kato, Shigeto Shimmura, Tetsuya Kawakita, Hideyuki Miyashita, Yoko Ogawa, Satoru Yoshida, Kazunari Higa, Hideyuki Okano, Kazuo Tsubota

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

PURPOSE. To investigate whether β-catenin activation and epithelial-mesenchymal transition (EMT) is involved in the pathogenesis of pterygium. METHODS. β-Catenin and E-cadherin expression were examined in surgically excised tissue and eye bank corneas with intact pterygium. Snail and Slug, the transcriptional repressors of E-cadherin, and matrix metalloproteinase (MMP)-7, a down-stream gene regulated by β-catenin were also investigated. Epithelial cells undergoing EMT-like changes were identified by double immunostaining for α-smooth muscle actin (SMA)/vimentin and cytokeratin 14. Transmission electron microscopy was used to examine the ultrastructure of the pterygial head. RESULTS. Histopathology showed aberrant fibrotic proliferation beneath the pterygium epithelium, with epithelial processes extending into the stroma. Transmission electron microscopy revealed the dissociation of epithelial cells, which were surrounded by activated fibroblast-like cells. Characteristic downregulation of E-cadherin and intranuclear accumulation of β-catenin and lymphoid-enhancer-factor-1 in pterygial epithelium were also observed by immunohistochemistry. Of note, epithelial cells extending into the stroma were positive for both α-SMA/vimentin and cytokeratin 14. Snail and Slug were immunopositive in the nuclei of pterygial epithelial cells, but not in normal corneal epithelial cells. CONCLUSIONS. EMT of basal epithelial cells may play a key role in the pathogenesis of pterygium.

Original languageEnglish
Pages (from-to)1511-1517
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume48
Issue number4
DOIs
Publication statusPublished - 2007 Apr

Fingerprint

Pterygium
Catenins
Epithelial-Mesenchymal Transition
Epithelial Cells
Cadherins
Keratin-14
Gastropoda
Vimentin
Transmission Electron Microscopy
Smooth Muscle
Actins
Epithelium
Eye Banks
Matrix Metalloproteinase 7
Tissue Banks
TCF Transcription Factors
Cornea
Down-Regulation
Fibroblasts
Immunohistochemistry

ASJC Scopus subject areas

  • Ophthalmology

Cite this

β-catenin activation and epithelial-mesenchymal transition in the pathogenesis of pterygium. / Kato, Naoko; Shimmura, Shigeto; Kawakita, Tetsuya; Miyashita, Hideyuki; Ogawa, Yoko; Yoshida, Satoru; Higa, Kazunari; Okano, Hideyuki; Tsubota, Kazuo.

In: Investigative Ophthalmology and Visual Science, Vol. 48, No. 4, 04.2007, p. 1511-1517.

Research output: Contribution to journalArticle

Kato, Naoko ; Shimmura, Shigeto ; Kawakita, Tetsuya ; Miyashita, Hideyuki ; Ogawa, Yoko ; Yoshida, Satoru ; Higa, Kazunari ; Okano, Hideyuki ; Tsubota, Kazuo. / β-catenin activation and epithelial-mesenchymal transition in the pathogenesis of pterygium. In: Investigative Ophthalmology and Visual Science. 2007 ; Vol. 48, No. 4. pp. 1511-1517.
@article{83d15c7d5c9746b0866aec0ab9f4c151,
title = "β-catenin activation and epithelial-mesenchymal transition in the pathogenesis of pterygium",
abstract = "PURPOSE. To investigate whether β-catenin activation and epithelial-mesenchymal transition (EMT) is involved in the pathogenesis of pterygium. METHODS. β-Catenin and E-cadherin expression were examined in surgically excised tissue and eye bank corneas with intact pterygium. Snail and Slug, the transcriptional repressors of E-cadherin, and matrix metalloproteinase (MMP)-7, a down-stream gene regulated by β-catenin were also investigated. Epithelial cells undergoing EMT-like changes were identified by double immunostaining for α-smooth muscle actin (SMA)/vimentin and cytokeratin 14. Transmission electron microscopy was used to examine the ultrastructure of the pterygial head. RESULTS. Histopathology showed aberrant fibrotic proliferation beneath the pterygium epithelium, with epithelial processes extending into the stroma. Transmission electron microscopy revealed the dissociation of epithelial cells, which were surrounded by activated fibroblast-like cells. Characteristic downregulation of E-cadherin and intranuclear accumulation of β-catenin and lymphoid-enhancer-factor-1 in pterygial epithelium were also observed by immunohistochemistry. Of note, epithelial cells extending into the stroma were positive for both α-SMA/vimentin and cytokeratin 14. Snail and Slug were immunopositive in the nuclei of pterygial epithelial cells, but not in normal corneal epithelial cells. CONCLUSIONS. EMT of basal epithelial cells may play a key role in the pathogenesis of pterygium.",
author = "Naoko Kato and Shigeto Shimmura and Tetsuya Kawakita and Hideyuki Miyashita and Yoko Ogawa and Satoru Yoshida and Kazunari Higa and Hideyuki Okano and Kazuo Tsubota",
year = "2007",
month = "4",
doi = "10.1167/iovs.06-1060",
language = "English",
volume = "48",
pages = "1511--1517",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "4",

}

TY - JOUR

T1 - β-catenin activation and epithelial-mesenchymal transition in the pathogenesis of pterygium

AU - Kato, Naoko

AU - Shimmura, Shigeto

AU - Kawakita, Tetsuya

AU - Miyashita, Hideyuki

AU - Ogawa, Yoko

AU - Yoshida, Satoru

AU - Higa, Kazunari

AU - Okano, Hideyuki

AU - Tsubota, Kazuo

PY - 2007/4

Y1 - 2007/4

N2 - PURPOSE. To investigate whether β-catenin activation and epithelial-mesenchymal transition (EMT) is involved in the pathogenesis of pterygium. METHODS. β-Catenin and E-cadherin expression were examined in surgically excised tissue and eye bank corneas with intact pterygium. Snail and Slug, the transcriptional repressors of E-cadherin, and matrix metalloproteinase (MMP)-7, a down-stream gene regulated by β-catenin were also investigated. Epithelial cells undergoing EMT-like changes were identified by double immunostaining for α-smooth muscle actin (SMA)/vimentin and cytokeratin 14. Transmission electron microscopy was used to examine the ultrastructure of the pterygial head. RESULTS. Histopathology showed aberrant fibrotic proliferation beneath the pterygium epithelium, with epithelial processes extending into the stroma. Transmission electron microscopy revealed the dissociation of epithelial cells, which were surrounded by activated fibroblast-like cells. Characteristic downregulation of E-cadherin and intranuclear accumulation of β-catenin and lymphoid-enhancer-factor-1 in pterygial epithelium were also observed by immunohistochemistry. Of note, epithelial cells extending into the stroma were positive for both α-SMA/vimentin and cytokeratin 14. Snail and Slug were immunopositive in the nuclei of pterygial epithelial cells, but not in normal corneal epithelial cells. CONCLUSIONS. EMT of basal epithelial cells may play a key role in the pathogenesis of pterygium.

AB - PURPOSE. To investigate whether β-catenin activation and epithelial-mesenchymal transition (EMT) is involved in the pathogenesis of pterygium. METHODS. β-Catenin and E-cadherin expression were examined in surgically excised tissue and eye bank corneas with intact pterygium. Snail and Slug, the transcriptional repressors of E-cadherin, and matrix metalloproteinase (MMP)-7, a down-stream gene regulated by β-catenin were also investigated. Epithelial cells undergoing EMT-like changes were identified by double immunostaining for α-smooth muscle actin (SMA)/vimentin and cytokeratin 14. Transmission electron microscopy was used to examine the ultrastructure of the pterygial head. RESULTS. Histopathology showed aberrant fibrotic proliferation beneath the pterygium epithelium, with epithelial processes extending into the stroma. Transmission electron microscopy revealed the dissociation of epithelial cells, which were surrounded by activated fibroblast-like cells. Characteristic downregulation of E-cadherin and intranuclear accumulation of β-catenin and lymphoid-enhancer-factor-1 in pterygial epithelium were also observed by immunohistochemistry. Of note, epithelial cells extending into the stroma were positive for both α-SMA/vimentin and cytokeratin 14. Snail and Slug were immunopositive in the nuclei of pterygial epithelial cells, but not in normal corneal epithelial cells. CONCLUSIONS. EMT of basal epithelial cells may play a key role in the pathogenesis of pterygium.

UR - http://www.scopus.com/inward/record.url?scp=34248384403&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34248384403&partnerID=8YFLogxK

U2 - 10.1167/iovs.06-1060

DO - 10.1167/iovs.06-1060

M3 - Article

C2 - 17389479

AN - SCOPUS:34248384403

VL - 48

SP - 1511

EP - 1517

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 4

ER -