β-catenin activation and epithelial-mesenchymal transition in the pathogenesis of pterygium

Naoko Kato, Shigeto Shimmura, Tetsuya Kawakita, Hideyuki Miyashita, Yoko Ogawa, Satoru Yoshida, Kazunari Higa, Hideyuki Okano, Kazuo Tsubota

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

PURPOSE. To investigate whether β-catenin activation and epithelial-mesenchymal transition (EMT) is involved in the pathogenesis of pterygium. METHODS. β-Catenin and E-cadherin expression were examined in surgically excised tissue and eye bank corneas with intact pterygium. Snail and Slug, the transcriptional repressors of E-cadherin, and matrix metalloproteinase (MMP)-7, a down-stream gene regulated by β-catenin were also investigated. Epithelial cells undergoing EMT-like changes were identified by double immunostaining for α-smooth muscle actin (SMA)/vimentin and cytokeratin 14. Transmission electron microscopy was used to examine the ultrastructure of the pterygial head. RESULTS. Histopathology showed aberrant fibrotic proliferation beneath the pterygium epithelium, with epithelial processes extending into the stroma. Transmission electron microscopy revealed the dissociation of epithelial cells, which were surrounded by activated fibroblast-like cells. Characteristic downregulation of E-cadherin and intranuclear accumulation of β-catenin and lymphoid-enhancer-factor-1 in pterygial epithelium were also observed by immunohistochemistry. Of note, epithelial cells extending into the stroma were positive for both α-SMA/vimentin and cytokeratin 14. Snail and Slug were immunopositive in the nuclei of pterygial epithelial cells, but not in normal corneal epithelial cells. CONCLUSIONS. EMT of basal epithelial cells may play a key role in the pathogenesis of pterygium.

Original languageEnglish
Pages (from-to)1511-1517
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume48
Issue number4
DOIs
Publication statusPublished - 2007 Apr 1

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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