β-Catenin Functions Pleiotropically in Differentiation and Tumorigenesis in Mouse Embryo-Derived Stem Cells

Noriko Okumura, Hidenori Akutsu, Tohru Sugawara, Takumi Miura, Youki Takezawa, Akihiro Hosoda, Keiichi Yoshida, Justin K. Ichida, Mitsutoshi Yamada, Toshio Hamatani, Naoaki Kuji, Kenji Miyado, Yasunori Yoshimura, Akihiro Umezawa

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The canonical Wnt/β-catenin signaling pathway plays a crucial role in the maintenance of the balance between proliferation and differentiation throughout embryogenesis and tissue homeostasis. β-Catenin, encoded by the Ctnnb1 gene, mediates an intracellular signaling cascade activated by Wnt. It also plays an important role in the maintenance of various types of stem cells including adult stem cells and cancer stem cells. However, it is unclear if β-catenin is required for the derivation of mouse embryo-derived stem cells. Here, we established β-catenin-deficient (β-catΔ/Δ) mouse embryo-derived stem cells and showed that β-catenin is not essential for acquiring self-renewal potential in the derivation of mouse embryonic stem cells (ESCs). However, teratomas formed from embryo-derived β-catΔ/Δ ESCs were immature germ cell tumors without multilineage differentiated cell types. Re-expression of functional β-catenin eliminated their neoplastic, transformed phenotype and restored pluripotency, thereby rescuing the mutant ESCs. Our findings demonstrate that β-catenin has pleiotropic effects in ESCs; it is required for the differentiation of ESCs and prevents them from acquiring tumorigenic character. These results highlight β-catenin as the gatekeeper in differentiation and tumorigenesis in ESCs.

Original languageEnglish
Article numbere63265
JournalPLoS One
Volume8
Issue number5
DOIs
Publication statusPublished - 2013 May 14

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Catenins
embryonic stem cells
Stem cells
carcinogenesis
stem cells
embryo (animal)
Carcinogenesis
Stem Cells
Embryonic Structures
Embryonic Stem Cells
mice
Maintenance
Tissue homeostasis
Wnt Signaling Pathway
Adult Stem Cells
Neoplastic Stem Cells
Germ Cell and Embryonal Neoplasms
germ cells
homeostasis
Teratoma

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Okumura, N., Akutsu, H., Sugawara, T., Miura, T., Takezawa, Y., Hosoda, A., ... Umezawa, A. (2013). β-Catenin Functions Pleiotropically in Differentiation and Tumorigenesis in Mouse Embryo-Derived Stem Cells. PLoS One, 8(5), [e63265]. https://doi.org/10.1371/journal.pone.0063265

β-Catenin Functions Pleiotropically in Differentiation and Tumorigenesis in Mouse Embryo-Derived Stem Cells. / Okumura, Noriko; Akutsu, Hidenori; Sugawara, Tohru; Miura, Takumi; Takezawa, Youki; Hosoda, Akihiro; Yoshida, Keiichi; Ichida, Justin K.; Yamada, Mitsutoshi; Hamatani, Toshio; Kuji, Naoaki; Miyado, Kenji; Yoshimura, Yasunori; Umezawa, Akihiro.

In: PLoS One, Vol. 8, No. 5, e63265, 14.05.2013.

Research output: Contribution to journalArticle

Okumura, N, Akutsu, H, Sugawara, T, Miura, T, Takezawa, Y, Hosoda, A, Yoshida, K, Ichida, JK, Yamada, M, Hamatani, T, Kuji, N, Miyado, K, Yoshimura, Y & Umezawa, A 2013, 'β-Catenin Functions Pleiotropically in Differentiation and Tumorigenesis in Mouse Embryo-Derived Stem Cells', PLoS One, vol. 8, no. 5, e63265. https://doi.org/10.1371/journal.pone.0063265
Okumura, Noriko ; Akutsu, Hidenori ; Sugawara, Tohru ; Miura, Takumi ; Takezawa, Youki ; Hosoda, Akihiro ; Yoshida, Keiichi ; Ichida, Justin K. ; Yamada, Mitsutoshi ; Hamatani, Toshio ; Kuji, Naoaki ; Miyado, Kenji ; Yoshimura, Yasunori ; Umezawa, Akihiro. / β-Catenin Functions Pleiotropically in Differentiation and Tumorigenesis in Mouse Embryo-Derived Stem Cells. In: PLoS One. 2013 ; Vol. 8, No. 5.
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