κ-Opioid agonist inhibits phospholipase C, possibly via an inhibition of G-protein activity

Hidemi Misawa, Hiroshi Ueda, Masamichi Satoh

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

In synaptic membranes of guinea pig cerebellum, in which preparations U-50, 488H, a κ-opioid agonist inhibits G-protein activity, the same κ-agonist at 100 nM inhibited the phospholipase C (PLC) activity stimulated by 100 μM GTP in the presence of 10 μM Ca2+. The present study provides a unique hypothetical mechanism that the receptor-mediated inhibition of PLC is mediated by inhibition of G-protein activity.

Original languageEnglish
Pages (from-to)324-327
Number of pages4
JournalNeuroscience Letters
Volume112
Issue number2-3
DOIs
Publication statusPublished - 1990 May 4
Externally publishedYes

Fingerprint

Type C Phospholipases
GTP-Binding Proteins
Opioid Analgesics
(trans)-Isomer 3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide
Synaptic Membranes
Guanosine Triphosphate
Cerebellum
Guinea Pigs

Keywords

  • GTP-binding protein
  • Phospholipase C
  • κ-Opioid receptor

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

κ-Opioid agonist inhibits phospholipase C, possibly via an inhibition of G-protein activity. / Misawa, Hidemi; Ueda, Hiroshi; Satoh, Masamichi.

In: Neuroscience Letters, Vol. 112, No. 2-3, 04.05.1990, p. 324-327.

Research output: Contribution to journalArticle

Misawa, Hidemi ; Ueda, Hiroshi ; Satoh, Masamichi. / κ-Opioid agonist inhibits phospholipase C, possibly via an inhibition of G-protein activity. In: Neuroscience Letters. 1990 ; Vol. 112, No. 2-3. pp. 324-327.
@article{892cb6bfeb0941408f3686835cb09a31,
title = "κ-Opioid agonist inhibits phospholipase C, possibly via an inhibition of G-protein activity",
abstract = "In synaptic membranes of guinea pig cerebellum, in which preparations U-50, 488H, a κ-opioid agonist inhibits G-protein activity, the same κ-agonist at 100 nM inhibited the phospholipase C (PLC) activity stimulated by 100 μM GTP in the presence of 10 μM Ca2+. The present study provides a unique hypothetical mechanism that the receptor-mediated inhibition of PLC is mediated by inhibition of G-protein activity.",
keywords = "GTP-binding protein, Phospholipase C, κ-Opioid receptor",
author = "Hidemi Misawa and Hiroshi Ueda and Masamichi Satoh",
year = "1990",
month = "5",
day = "4",
doi = "10.1016/0304-3940(90)90224-W",
language = "English",
volume = "112",
pages = "324--327",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "2-3",

}

TY - JOUR

T1 - κ-Opioid agonist inhibits phospholipase C, possibly via an inhibition of G-protein activity

AU - Misawa, Hidemi

AU - Ueda, Hiroshi

AU - Satoh, Masamichi

PY - 1990/5/4

Y1 - 1990/5/4

N2 - In synaptic membranes of guinea pig cerebellum, in which preparations U-50, 488H, a κ-opioid agonist inhibits G-protein activity, the same κ-agonist at 100 nM inhibited the phospholipase C (PLC) activity stimulated by 100 μM GTP in the presence of 10 μM Ca2+. The present study provides a unique hypothetical mechanism that the receptor-mediated inhibition of PLC is mediated by inhibition of G-protein activity.

AB - In synaptic membranes of guinea pig cerebellum, in which preparations U-50, 488H, a κ-opioid agonist inhibits G-protein activity, the same κ-agonist at 100 nM inhibited the phospholipase C (PLC) activity stimulated by 100 μM GTP in the presence of 10 μM Ca2+. The present study provides a unique hypothetical mechanism that the receptor-mediated inhibition of PLC is mediated by inhibition of G-protein activity.

KW - GTP-binding protein

KW - Phospholipase C

KW - κ-Opioid receptor

UR - http://www.scopus.com/inward/record.url?scp=0025699766&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025699766&partnerID=8YFLogxK

U2 - 10.1016/0304-3940(90)90224-W

DO - 10.1016/0304-3940(90)90224-W

M3 - Article

C2 - 2163041

AN - SCOPUS:0025699766

VL - 112

SP - 324

EP - 327

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 2-3

ER -