10 nm spatial resolution fluorescence imaging of single molecules by near-field scanning optical microscopy using a tiny aperture probe

Noriko Hosaka, Toshiharu Saiki

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We demonstrate high-resolution fluorescence imaging of single molecules using near-field scanning optical microscopy (NSOM) with a tiny aperture probe for two different wavelengths in visible range in the illumination mode of operation. The spatial resolutions obtained at both excitation wavelengths were almost the same and the highest resolution realized was about 10 nm. To discuss the achievable resolution in aperture NSOM, we also employed a computer simulation by the finite-difference time-domain method for various aperture sizes and wavelengths. The resolution of 10 nm is predicted to be contributed by the single peak of localized near-field light around the rim of the aperture.

Original languageEnglish
Pages (from-to)262-265
Number of pages4
JournalOptical Review
Volume13
Issue number4
DOIs
Publication statusPublished - 2006 Jul

Fingerprint

near fields
spatial resolution
apertures
microscopy
fluorescence
scanning
probes
molecules
wavelengths
high resolution
rims
finite difference time domain method
computerized simulation
illumination
excitation

Keywords

  • Aperture probe
  • Finite-difference time-domain (FDTD) method
  • Fluorescence imaging
  • High spatial resolution
  • Near-field scanning optical microscopy (NSOM)
  • Single molecule

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

10 nm spatial resolution fluorescence imaging of single molecules by near-field scanning optical microscopy using a tiny aperture probe. / Hosaka, Noriko; Saiki, Toshiharu.

In: Optical Review, Vol. 13, No. 4, 07.2006, p. 262-265.

Research output: Contribution to journalArticle

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