16S rRNA gene-based analysis of microbial community by whole-genome amplification and minigel-single-strand conformation polymorphism technique

Michiei Oto, Wataru Suda, Hirofumi Shinoyama

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We have developed an analytical technique for the 16S rRNA gene that comprises whole-genome amplification and the polymerase chain reaction (PCR)-minigel-single-strand conformation polymorphism technique (WGA-SSCP). Under optimal conditions, SSCP bands could be detected when genomic DNA from bacteria of interest comprised 0.5% or more of the specimen. This method will be effective for the identification of nonculturable bacteria in a microbial community.

Original languageEnglish
Pages (from-to)482-484
Number of pages3
JournalJournal of Bioscience and Bioengineering
Volume102
Issue number5
DOIs
Publication statusPublished - 2006 Nov 1

Keywords

  • 16S rRNA gene
  • nonculturable bacteria
  • polymerase chain reaction (PCR)
  • single-strand conformation polymorphism (SSCP)
  • whole-genome amplification (WGA)

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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