We have developed an analytical technique for the 16S rRNA gene that comprises whole-genome amplification and the polymerase chain reaction (PCR)-minigel-single-strand conformation polymorphism technique (WGA-SSCP). Under optimal conditions, SSCP bands could be detected when genomic DNA from bacteria of interest comprised 0.5% or more of the specimen. This method will be effective for the identification of nonculturable bacteria in a microbial community.
- 16S rRNA gene
- nonculturable bacteria
- polymerase chain reaction (PCR)
- single-strand conformation polymorphism (SSCP)
- whole-genome amplification (WGA)
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology