17α, 20β-dihydroxy-4-pregnen-3-one induced substances, trypsin and 11β-hydroxy steroid dehydrogenase, are important factors for control of spermatogenesis in Japanese eel

Chiemi Miura, Yuich Ozaki, Masato Higuchi, Takashi Ohta, Takeshi Miura

Research output: Contribution to journalArticle

Abstract

Using cDNA subtraction and differential screening, we identified 25 genes that were differentially expressed by 17α, 20β-dihydroxy-4-pregnen-3- one (DHP) stimulation. Among these clones, eel trypsin and eel 11β-hydroxysteroid dehydrogenase short form (e11 β-HSDsf) were analyzed. The results indicate that transcripts of eel trypsin and e11β-HSD in testis were induced by DHP stimulation. In vitro experiments revealed that trypsin induces initiation of meiosis and spermiogenesis. Moreover, Cortisol enhanced the 11-ketotestesterone (HKT)-induced spermatogonial proliferation. From these results, we conclude that these two molecules are important for controlling spermatogenesis.

Original languageEnglish
Pages (from-to)164
Number of pages1
JournalCybium
Volume32
Issue number2 SUPPL.
Publication statusPublished - 2008 Jul 31
Externally publishedYes

Fingerprint

Anguilla japonica
steroid
eel
spermatogenesis
trypsin
steroids
hydroxysteroid dehydrogenases
spermiogenesis
meiosis
cortisol
clone
testes
clones
screening
gene
genes
experiment

Keywords

  • In vitro culture
  • Meiosis
  • Spermatogonial proliferation
  • Spermiogenesis

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Animal Science and Zoology

Cite this

17α, 20β-dihydroxy-4-pregnen-3-one induced substances, trypsin and 11β-hydroxy steroid dehydrogenase, are important factors for control of spermatogenesis in Japanese eel. / Miura, Chiemi; Ozaki, Yuich; Higuchi, Masato; Ohta, Takashi; Miura, Takeshi.

In: Cybium, Vol. 32, No. 2 SUPPL., 31.07.2008, p. 164.

Research output: Contribution to journalArticle

Miura, Chiemi ; Ozaki, Yuich ; Higuchi, Masato ; Ohta, Takashi ; Miura, Takeshi. / 17α, 20β-dihydroxy-4-pregnen-3-one induced substances, trypsin and 11β-hydroxy steroid dehydrogenase, are important factors for control of spermatogenesis in Japanese eel. In: Cybium. 2008 ; Vol. 32, No. 2 SUPPL. pp. 164.
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