2-Methoxy-1,4-naphthoquinone isolated from Impatiens balsamina in a screening program for activity to inhibit Wnt signaling

Naomi Mori; Kazufumi Toume; Midori A. Arai; Takashi Koyano; Thaworn Kowithayakorn; Masami Ishibashi

doi:10.1007/s11418-010-0471-0

2-Methoxy-1,4-naphthoquinone isolated from Impatiens balsamina in a screening program for activity to inhibit Wnt signaling

Naomi Mori, Kazufumi Toume, Midori A. Arai, Takashi Koyano, Thaworn Kowithayakorn, Masami Ishibashi

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

A screening study using a luciferase assay to identify natural products which inhibit Wnt signaling was carried out. The bioassay-guided fractionation of aerial parts of a plant, Impatiens balsamina, led to the isolation of 2-methoxy-1,4-naphthoquinone (1) as an active compound. Compound 1 inhibited the TCF/β-catenin (TOP) transcriptional activity (IC₅₀ 2.9 μM), while it decreased the transcriptional activity of FOP (mutated TCF-binding site)-transfected cells at >5 μM.

Original language	English
Pages (from-to)	234-236
Number of pages	3
Journal	Journal of Natural Medicines
Volume	65
Issue number	1
DOIs	https://doi.org/10.1007/s11418-010-0471-0
Publication status	Published - 2011 Jan
Externally published	Yes

Keywords

Impatiens balsamina
Luciferase
Naphthoquinone
Wnt

ASJC Scopus subject areas

Molecular Medicine

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10.1007/s11418-010-0471-0

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title = "2-Methoxy-1,4-naphthoquinone isolated from Impatiens balsamina in a screening program for activity to inhibit Wnt signaling",

abstract = "A screening study using a luciferase assay to identify natural products which inhibit Wnt signaling was carried out. The bioassay-guided fractionation of aerial parts of a plant, Impatiens balsamina, led to the isolation of 2-methoxy-1,4-naphthoquinone (1) as an active compound. Compound 1 inhibited the TCF/β-catenin (TOP) transcriptional activity (IC50 2.9 μM), while it decreased the transcriptional activity of FOP (mutated TCF-binding site)-transfected cells at >5 μM.",

keywords = "Impatiens balsamina, Luciferase, Naphthoquinone, Wnt",

author = "Naomi Mori and Kazufumi Toume and Arai, {Midori A.} and Takashi Koyano and Thaworn Kowithayakorn and Masami Ishibashi",

note = "Funding Information: Acknowledgments We are grateful to Prof. J. Nathans (John Hopkins Medical School) for the STF/293 cells and Prof. R. T. Moon (University of Washington) for the SuperTOP-Flash and SuperFOP-Flash plasmids. This work was supported by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS), the Japan Science and Technology Agency (JST), and the Chiba Foundation for Health Promotion and Disease Prevention.",

year = "2011",

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doi = "10.1007/s11418-010-0471-0",

language = "English",

volume = "65",

pages = "234--236",

journal = "Journal of Natural Medicines",

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AU - Mori, Naomi

AU - Toume, Kazufumi

AU - Arai, Midori A.

AU - Koyano, Takashi

AU - Kowithayakorn, Thaworn

AU - Ishibashi, Masami

N1 - Funding Information: Acknowledgments We are grateful to Prof. J. Nathans (John Hopkins Medical School) for the STF/293 cells and Prof. R. T. Moon (University of Washington) for the SuperTOP-Flash and SuperFOP-Flash plasmids. This work was supported by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS), the Japan Science and Technology Agency (JST), and the Chiba Foundation for Health Promotion and Disease Prevention.

PY - 2011/1

Y1 - 2011/1

N2 - A screening study using a luciferase assay to identify natural products which inhibit Wnt signaling was carried out. The bioassay-guided fractionation of aerial parts of a plant, Impatiens balsamina, led to the isolation of 2-methoxy-1,4-naphthoquinone (1) as an active compound. Compound 1 inhibited the TCF/β-catenin (TOP) transcriptional activity (IC50 2.9 μM), while it decreased the transcriptional activity of FOP (mutated TCF-binding site)-transfected cells at >5 μM.

AB - A screening study using a luciferase assay to identify natural products which inhibit Wnt signaling was carried out. The bioassay-guided fractionation of aerial parts of a plant, Impatiens balsamina, led to the isolation of 2-methoxy-1,4-naphthoquinone (1) as an active compound. Compound 1 inhibited the TCF/β-catenin (TOP) transcriptional activity (IC50 2.9 μM), while it decreased the transcriptional activity of FOP (mutated TCF-binding site)-transfected cells at >5 μM.

KW - Impatiens balsamina

KW - Luciferase

KW - Naphthoquinone

KW - Wnt

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2-Methoxy-1,4-naphthoquinone isolated from Impatiens balsamina in a screening program for activity to inhibit Wnt signaling

Abstract

Keywords

ASJC Scopus subject areas

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