3′-Untranslated region of doublecortin mRNA is a binding target of the Musashi1 RNA-binding protein

Kenichi Horisawa, Takao Imai, Hideyuki Okano, Hiroshi Yanagawa

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Musashi1 (Msi1) is an RNA-binding protein that is highly expressed in neural stem cells, and is considered to be a stemness factor. A known function of Msi1 is translational repression of specifically bound mRNAs. Although the basic mechanism and some target RNAs have been reported, further survey of interactors is necessary to understand the integrated function of Msi1. By screening using an mRNA display technique, we found that doublecortin (dcx) mRNA is a specific binding target of Msi1 in vitro. We confirmed that Msil repressed translation of a luciferase reporter gene linked to the selected 3′-untranslated region fragment of dcx in Neuro2A cells.

Original languageEnglish
Pages (from-to)2429-2434
Number of pages6
JournalFEBS Letters
Volume583
Issue number14
DOIs
Publication statusPublished - 2009 Jul 21

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Keywords

  • 3′-Untranslated region
  • Doublecortin
  • Musashi1
  • Neural stem cell
  • RNA-binding protein
  • Translational repression

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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