3D culture of mouse iPSCs in hydrogel core-shell microfibers

Kazuhiro Ikeda, Teru Okitsu, Hiroaki Onoe, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports the culturing and expansion of mouse induced pluripotent stem cells (iPSCs) in hydrogel core-shell microfibers; the core consists of iPSCs with or without extracellular matrix (ECM) proteins, and the shell is composed of calcium alginate. We revealed that mouse iPSCs cultured in the micro-scale space with ECM proteins sustain their pluriotency efficiently. This 3D culture system may be a useful tool to expand iPSCs for clinical use.

Original languageEnglish
Title of host publicationProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages463-464
Number of pages2
Volume2015-February
EditionFebruary
DOIs
Publication statusPublished - 2015 Feb 26
Externally publishedYes
Event2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015 - Estoril, Portugal
Duration: 2015 Jan 182015 Jan 22

Other

Other2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015
CountryPortugal
CityEstoril
Period15/1/1815/1/22

Fingerprint

microfibers
stem cells
Hydrogel
Stem cells
Cell culture
Hydrogels
mice
Extracellular Matrix Proteins
proteins
Proteins
Alginate
matrices
calcium
Calcium
expansion

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

Ikeda, K., Okitsu, T., Onoe, H., & Takeuchi, S. (2015). 3D culture of mouse iPSCs in hydrogel core-shell microfibers. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) (February ed., Vol. 2015-February, pp. 463-464). [7050990] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2015.7050990

3D culture of mouse iPSCs in hydrogel core-shell microfibers. / Ikeda, Kazuhiro; Okitsu, Teru; Onoe, Hiroaki; Takeuchi, Shoji.

Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). Vol. 2015-February February. ed. Institute of Electrical and Electronics Engineers Inc., 2015. p. 463-464 7050990.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ikeda, K, Okitsu, T, Onoe, H & Takeuchi, S 2015, 3D culture of mouse iPSCs in hydrogel core-shell microfibers. in Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). February edn, vol. 2015-February, 7050990, Institute of Electrical and Electronics Engineers Inc., pp. 463-464, 2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015, Estoril, Portugal, 15/1/18. https://doi.org/10.1109/MEMSYS.2015.7050990
Ikeda K, Okitsu T, Onoe H, Takeuchi S. 3D culture of mouse iPSCs in hydrogel core-shell microfibers. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). February ed. Vol. 2015-February. Institute of Electrical and Electronics Engineers Inc. 2015. p. 463-464. 7050990 https://doi.org/10.1109/MEMSYS.2015.7050990
Ikeda, Kazuhiro ; Okitsu, Teru ; Onoe, Hiroaki ; Takeuchi, Shoji. / 3D culture of mouse iPSCs in hydrogel core-shell microfibers. Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). Vol. 2015-February February. ed. Institute of Electrical and Electronics Engineers Inc., 2015. pp. 463-464
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