3D culture of mouse iPSCs in hydrogel core-shell microfibers

Kazuhiro Ikeda, Teru Okitsu, Hiroaki Onoe, Shoji Takeuchi

Research output: Contribution to journalConference article

Abstract

This paper reports the culturing and expansion of mouse induced pluripotent stem cells (iPSCs) in hydrogel core-shell microfibers; the core consists of iPSCs with or without extracellular matrix (ECM) proteins, and the shell is composed of calcium alginate. We revealed that mouse iPSCs cultured in the micro-scale space with ECM proteins sustain their pluriotency efficiently. This 3D culture system may be a useful tool to expand iPSCs for clinical use.

Original languageEnglish
Article number7050990
Pages (from-to)463-464
Number of pages2
JournalProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume2015-February
Issue numberFebruary
DOIs
Publication statusPublished - 2015 Feb 26
Externally publishedYes
Event2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015 - Estoril, Portugal
Duration: 2015 Jan 182015 Jan 22

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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