3D fiber-shaped culture system promotes differentiation of multi potent DFAT cells into smooth muscle-like cells

A. Y. Hsiao, T. Okitsu, H. Onoe, M. Kiyosawa, H. Teramae, S. Iwanaga, S. Miura, T. Kazama, T. Matsumoto, S. Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Here we show that 3D fiber-shaped culture system enhances the differentiation of multipotent DFAT (dedifferentiated fat) cells into smooth muscle-like cells. DFAT cells cultured as 3D cell fiber constructs were induced to differentiate into smooth muscle cells. We found expression of smooth muscle lineage specific markers ASMA ( α-smooth muscle actin) and calponin in the differentiated DFAT cell fibers. The expression of calponin as marker of more mature smooth muscle cells has not been achieved in 2D cultures before. These findings suggest that 3D fiber microenvironment is able to promote more efficient differentiation into smooth muscle-like cells compared to 2D culture.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages410-412
Number of pages3
ISBN (Print)9781632666246
Publication statusPublished - 2013 Jan 1
Externally publishedYes
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 2013 Oct 272013 Oct 31

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume1

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period13/10/2713/10/31

Keywords

  • 3D culture
  • Differentiation
  • Microfluidic
  • Smooth muscle

ASJC Scopus subject areas

  • Bioengineering

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  • Cite this

    Hsiao, A. Y., Okitsu, T., Onoe, H., Kiyosawa, M., Teramae, H., Iwanaga, S., Miura, S., Kazama, T., Matsumoto, T., & Takeuchi, S. (2013). 3D fiber-shaped culture system promotes differentiation of multi potent DFAT cells into smooth muscle-like cells. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 410-412). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 1). Chemical and Biological Microsystems Society.