3D fiber-shaped culture system promotes differentiation of multi potent DFAT cells into smooth muscle-like cells

A. Y. Hsiao, T. Okitsu, Hiroaki Onoe, M. Kiyosawa, H. Teramae, S. Iwanaga, S. Miura, T. Kazama, T. Matsumoto, S. Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Here we show that 3D fiber-shaped culture system enhances the differentiation of multipotent DFAT (dedifferentiated fat) cells into smooth muscle-like cells. DFAT cells cultured as 3D cell fiber constructs were induced to differentiate into smooth muscle cells. We found expression of smooth muscle lineage specific markers ASMA ( α-smooth muscle actin) and calponin in the differentiated DFAT cell fibers. The expression of calponin as marker of more mature smooth muscle cells has not been achieved in 2D cultures before. These findings suggest that 3D fiber microenvironment is able to promote more efficient differentiation into smooth muscle-like cells compared to 2D culture.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages410-412
Number of pages3
Volume1
ISBN (Print)9781632666246
Publication statusPublished - 2013
Externally publishedYes
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 2013 Oct 272013 Oct 31

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period13/10/2713/10/31

Fingerprint

Oils and fats
Muscle
Fats
Fibers
Cells
Cell culture
Actins

Keywords

  • 3D culture
  • Differentiation
  • Microfluidic
  • Smooth muscle

ASJC Scopus subject areas

  • Bioengineering

Cite this

Hsiao, A. Y., Okitsu, T., Onoe, H., Kiyosawa, M., Teramae, H., Iwanaga, S., ... Takeuchi, S. (2013). 3D fiber-shaped culture system promotes differentiation of multi potent DFAT cells into smooth muscle-like cells. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (Vol. 1, pp. 410-412). Chemical and Biological Microsystems Society.

3D fiber-shaped culture system promotes differentiation of multi potent DFAT cells into smooth muscle-like cells. / Hsiao, A. Y.; Okitsu, T.; Onoe, Hiroaki; Kiyosawa, M.; Teramae, H.; Iwanaga, S.; Miura, S.; Kazama, T.; Matsumoto, T.; Takeuchi, S.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 1 Chemical and Biological Microsystems Society, 2013. p. 410-412.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Hsiao, AY, Okitsu, T, Onoe, H, Kiyosawa, M, Teramae, H, Iwanaga, S, Miura, S, Kazama, T, Matsumoto, T & Takeuchi, S 2013, 3D fiber-shaped culture system promotes differentiation of multi potent DFAT cells into smooth muscle-like cells. in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. vol. 1, Chemical and Biological Microsystems Society, pp. 410-412, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Germany, 13/10/27.
Hsiao AY, Okitsu T, Onoe H, Kiyosawa M, Teramae H, Iwanaga S et al. 3D fiber-shaped culture system promotes differentiation of multi potent DFAT cells into smooth muscle-like cells. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 1. Chemical and Biological Microsystems Society. 2013. p. 410-412
Hsiao, A. Y. ; Okitsu, T. ; Onoe, Hiroaki ; Kiyosawa, M. ; Teramae, H. ; Iwanaga, S. ; Miura, S. ; Kazama, T. ; Matsumoto, T. ; Takeuchi, S. / 3D fiber-shaped culture system promotes differentiation of multi potent DFAT cells into smooth muscle-like cells. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 1 Chemical and Biological Microsystems Society, 2013. pp. 410-412
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