3D localized 1H-13C heteronuclear single-quantum coherence correlation spectroscopy in vivo

H. Watanabe, Y. Ishihara, K. Okamoto, Koichi Oshio, T. Kanamatsu, Y. Tsukada

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A method for spatially three-dimensional (3D) localized two-dimensional (2D) 1H-13C correlation spectroscopy, localized HSQC, is proposed. This method has the following special feature in the preparation period. The 180°(13C) and 180°(1H) pulses are separated in time, and the 180°(13C) pulse is applied at 1/(4 1J(CH)) before the 90°(1H) polarization transfer pulse. The preparation (echo) period 2τ can then be set substantially longer than 1/(2 1J(CH)), so that even in a whole-body system, slice-selective 90°(1H) pulses and gradient pulses can be applied in that period. The localization capabilities of this method were confirmed in a phantom experiment. The 3D localized 2D 1H-13C correlation spectra from a monkey brain in vivo were obtained after [1-13C]glucose injection, and amino acid metabolism was detected; that is, [4-13C]glutamate appeared immediately after the injection, followed by the appearance of [2- 13C]glutamate, [3-13C]glutamate, and [4-13C]glutamine. (C) 2000 Wiley- Liss, Inc.

Original languageEnglish
Pages (from-to)200-210
Number of pages11
JournalMagnetic Resonance in Medicine
Volume43
Issue number2
DOIs
Publication statusPublished - 2000
Externally publishedYes

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Glutamic Acid
Spectrum Analysis
Injections
Glutamine
Haplorhini
Amino Acids
Glucose
Brain

Keywords

  • H-C HSQC correlation
  • Amino acid metabolism
  • In vivo
  • MRS
  • Spatial localization

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

3D localized 1H-13C heteronuclear single-quantum coherence correlation spectroscopy in vivo. / Watanabe, H.; Ishihara, Y.; Okamoto, K.; Oshio, Koichi; Kanamatsu, T.; Tsukada, Y.

In: Magnetic Resonance in Medicine, Vol. 43, No. 2, 2000, p. 200-210.

Research output: Contribution to journalArticle

Watanabe, H. ; Ishihara, Y. ; Okamoto, K. ; Oshio, Koichi ; Kanamatsu, T. ; Tsukada, Y. / 3D localized 1H-13C heteronuclear single-quantum coherence correlation spectroscopy in vivo. In: Magnetic Resonance in Medicine. 2000 ; Vol. 43, No. 2. pp. 200-210.
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