TY - JOUR
T1 - 3D localized 1H-13C heteronuclear single-quantum coherence correlation spectroscopy in vivo
AU - Watanabe, H.
AU - Ishihara, Y.
AU - Okamoto, K.
AU - Oshio, K.
AU - Kanamatsu, T.
AU - Tsukada, Y.
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2000
Y1 - 2000
N2 - A method for spatially three-dimensional (3D) localized two-dimensional (2D) 1H-13C correlation spectroscopy, localized HSQC, is proposed. This method has the following special feature in the preparation period. The 180°(13C) and 180°(1H) pulses are separated in time, and the 180°(13C) pulse is applied at 1/(4 1J(CH)) before the 90°(1H) polarization transfer pulse. The preparation (echo) period 2τ can then be set substantially longer than 1/(2 1J(CH)), so that even in a whole-body system, slice-selective 90°(1H) pulses and gradient pulses can be applied in that period. The localization capabilities of this method were confirmed in a phantom experiment. The 3D localized 2D 1H-13C correlation spectra from a monkey brain in vivo were obtained after [1-13C]glucose injection, and amino acid metabolism was detected; that is, [4-13C]glutamate appeared immediately after the injection, followed by the appearance of [2- 13C]glutamate, [3-13C]glutamate, and [4-13C]glutamine. (C) 2000 Wiley- Liss, Inc.
AB - A method for spatially three-dimensional (3D) localized two-dimensional (2D) 1H-13C correlation spectroscopy, localized HSQC, is proposed. This method has the following special feature in the preparation period. The 180°(13C) and 180°(1H) pulses are separated in time, and the 180°(13C) pulse is applied at 1/(4 1J(CH)) before the 90°(1H) polarization transfer pulse. The preparation (echo) period 2τ can then be set substantially longer than 1/(2 1J(CH)), so that even in a whole-body system, slice-selective 90°(1H) pulses and gradient pulses can be applied in that period. The localization capabilities of this method were confirmed in a phantom experiment. The 3D localized 2D 1H-13C correlation spectra from a monkey brain in vivo were obtained after [1-13C]glucose injection, and amino acid metabolism was detected; that is, [4-13C]glutamate appeared immediately after the injection, followed by the appearance of [2- 13C]glutamate, [3-13C]glutamate, and [4-13C]glutamine. (C) 2000 Wiley- Liss, Inc.
KW - Amino acid metabolism
KW - H-C HSQC correlation
KW - In vivo
KW - MRS
KW - Spatial localization
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U2 - 10.1002/(SICI)1522-2594(200002)43:2<200::AID-MRM6>3.0.CO;2-H
DO - 10.1002/(SICI)1522-2594(200002)43:2<200::AID-MRM6>3.0.CO;2-H
M3 - Article
C2 - 10680683
AN - SCOPUS:0033621635
SN - 0740-3194
VL - 43
SP - 200
EP - 210
JO - Magnetic Resonance in Medicine
JF - Magnetic Resonance in Medicine
IS - 2
ER -