3D visualization of epidermal langerhans cells

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Citations (Scopus)

Abstract

Langerhans cells (LCs) are intraepidermal dendritic cells that extend their dendrites between keratinocytes to form a dense network that covers the entire body. The mammalian epidermis has two diffusion barriers, the stratum corneum and tight junctions (TJs). In their resting state, LCs sit underneath these two barriers. Once activated, LCs elongate their dendrites to dock with, or penetrate through, TJs, which allows them to survey the environment between the two barriers. Here, we describe a method to visualize this dynamic interaction of TJs and LCs in 3D by using mouse ear epidermal sheets.

Original languageEnglish
Title of host publicationMolecular Dermatology
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages119-127
Number of pages9
ISBN (Print)9781627032261
DOIs
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology
Volume961
ISSN (Print)1064-3745

    Fingerprint

Keywords

  • Langerhans cells
  • Skin-resident dendritic cells
  • Stratum corneum
  • Tight junction

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Kubo, A., Nagao, K., & Amagai, M. (2013). 3D visualization of epidermal langerhans cells. In Molecular Dermatology: Methods and Protocols (pp. 119-127). (Methods in Molecular Biology; Vol. 961). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-227-8_5