5-Hydroxytryptamine strongly inhibits fluid secretion in guinea pig pancreatic duct cells

Atsushi Suzuki, Satoru Naruse, Motoji Kitagawa, Hiroshi Ishiguro, Toshiyuki Yoshikawa, Shigeru Ko, Akiko Yamamoto, Hiroyuki Hamada, Tetsuo Hayakawa

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

We studied the distribution of 5-hydroxytryptamine- (5-HT-) containing cells in the guinea pig pancreas and examined the effects of 5-HT on fluid secretion by interlobular pancreatic ducts. The 5-HT-immunoreactive cells with morphological characteristics of enterochromaffin (EC) cells were scattered throughout the duct system and were enriched in islets of Langerhans. The fluid secretory rate in the isolated interlobular ducts was measured by videomicroscopy. Basolateral applications of 5-HT strongly but reversibly reduced HCO3-dependent, as well as secretin- and acetylcholine- (ACh-) stimulated, fluid secretion, whereas 5-HT applied into the lumen had no such effects. Secretin-stimulated fluid secretion could be inhibited by a 5-HT3 receptor agonist, but not by agonists of the 5-HT1, 5-HT2, or 5-HT4 receptors. Under the stimulation with secretin, 5-HT decreased the intracellular pH (pHi) and reduced the rate of pHi recovery after acid loading with NH4+, suggesting that 5-HT inhibits the intracellular accumulation of HCO3-. The elevation of intraductal pressure in vivo reduced secretin-stimulated fluid secretion, an effect that could be attenuated by a 5-HT3 receptor antagonist. Thus, 5-HT, acting through basolateral 5-HT3 receptors, strongly inhibits spontaneous, secretin-, and ACh-stimulated fluid secretion by guinea pig pancreatic ducts. 5-HT released from pancreatic ductal EC cells on elevation of the intraductal pressure may regulate fluid secretion of neighboring duct cells in a paracrine fashion.

Original languageEnglish
Pages (from-to)749-756
Number of pages8
JournalJournal of Clinical Investigation
Volume108
Issue number5
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Fluids and Secretions
Pancreatic Ducts
Serotonin
Guinea Pigs
Secretin
Receptors, Serotonin, 5-HT3
Enterochromaffin Cells
Acetylcholine
Serotonin 5-HT3 Receptor Agonists
Secretory Rate
Receptors, Serotonin, 5-HT4
Serotonin 5-HT1 Receptor Agonists
Serotonin 5-HT3 Receptor Antagonists
Pressure
Video Microscopy
Islets of Langerhans
Pancreas
Acids

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Suzuki, A., Naruse, S., Kitagawa, M., Ishiguro, H., Yoshikawa, T., Ko, S., ... Hayakawa, T. (2001). 5-Hydroxytryptamine strongly inhibits fluid secretion in guinea pig pancreatic duct cells. Journal of Clinical Investigation, 108(5), 749-756. https://doi.org/10.1172/JCI200112312

5-Hydroxytryptamine strongly inhibits fluid secretion in guinea pig pancreatic duct cells. / Suzuki, Atsushi; Naruse, Satoru; Kitagawa, Motoji; Ishiguro, Hiroshi; Yoshikawa, Toshiyuki; Ko, Shigeru; Yamamoto, Akiko; Hamada, Hiroyuki; Hayakawa, Tetsuo.

In: Journal of Clinical Investigation, Vol. 108, No. 5, 2001, p. 749-756.

Research output: Contribution to journalArticle

Suzuki, A, Naruse, S, Kitagawa, M, Ishiguro, H, Yoshikawa, T, Ko, S, Yamamoto, A, Hamada, H & Hayakawa, T 2001, '5-Hydroxytryptamine strongly inhibits fluid secretion in guinea pig pancreatic duct cells', Journal of Clinical Investigation, vol. 108, no. 5, pp. 749-756. https://doi.org/10.1172/JCI200112312
Suzuki, Atsushi ; Naruse, Satoru ; Kitagawa, Motoji ; Ishiguro, Hiroshi ; Yoshikawa, Toshiyuki ; Ko, Shigeru ; Yamamoto, Akiko ; Hamada, Hiroyuki ; Hayakawa, Tetsuo. / 5-Hydroxytryptamine strongly inhibits fluid secretion in guinea pig pancreatic duct cells. In: Journal of Clinical Investigation. 2001 ; Vol. 108, No. 5. pp. 749-756.
@article{88056417370644ed98860720ba7ca96b,
title = "5-Hydroxytryptamine strongly inhibits fluid secretion in guinea pig pancreatic duct cells",
abstract = "We studied the distribution of 5-hydroxytryptamine- (5-HT-) containing cells in the guinea pig pancreas and examined the effects of 5-HT on fluid secretion by interlobular pancreatic ducts. The 5-HT-immunoreactive cells with morphological characteristics of enterochromaffin (EC) cells were scattered throughout the duct system and were enriched in islets of Langerhans. The fluid secretory rate in the isolated interlobular ducts was measured by videomicroscopy. Basolateral applications of 5-HT strongly but reversibly reduced HCO3-dependent, as well as secretin- and acetylcholine- (ACh-) stimulated, fluid secretion, whereas 5-HT applied into the lumen had no such effects. Secretin-stimulated fluid secretion could be inhibited by a 5-HT3 receptor agonist, but not by agonists of the 5-HT1, 5-HT2, or 5-HT4 receptors. Under the stimulation with secretin, 5-HT decreased the intracellular pH (pHi) and reduced the rate of pHi recovery after acid loading with NH4+, suggesting that 5-HT inhibits the intracellular accumulation of HCO3-. The elevation of intraductal pressure in vivo reduced secretin-stimulated fluid secretion, an effect that could be attenuated by a 5-HT3 receptor antagonist. Thus, 5-HT, acting through basolateral 5-HT3 receptors, strongly inhibits spontaneous, secretin-, and ACh-stimulated fluid secretion by guinea pig pancreatic ducts. 5-HT released from pancreatic ductal EC cells on elevation of the intraductal pressure may regulate fluid secretion of neighboring duct cells in a paracrine fashion.",
author = "Atsushi Suzuki and Satoru Naruse and Motoji Kitagawa and Hiroshi Ishiguro and Toshiyuki Yoshikawa and Shigeru Ko and Akiko Yamamoto and Hiroyuki Hamada and Tetsuo Hayakawa",
year = "2001",
doi = "10.1172/JCI200112312",
language = "English",
volume = "108",
pages = "749--756",
journal = "Journal of Clinical Investigation",
issn = "0021-9738",
publisher = "The American Society for Clinical Investigation",
number = "5",

}

TY - JOUR

T1 - 5-Hydroxytryptamine strongly inhibits fluid secretion in guinea pig pancreatic duct cells

AU - Suzuki, Atsushi

AU - Naruse, Satoru

AU - Kitagawa, Motoji

AU - Ishiguro, Hiroshi

AU - Yoshikawa, Toshiyuki

AU - Ko, Shigeru

AU - Yamamoto, Akiko

AU - Hamada, Hiroyuki

AU - Hayakawa, Tetsuo

PY - 2001

Y1 - 2001

N2 - We studied the distribution of 5-hydroxytryptamine- (5-HT-) containing cells in the guinea pig pancreas and examined the effects of 5-HT on fluid secretion by interlobular pancreatic ducts. The 5-HT-immunoreactive cells with morphological characteristics of enterochromaffin (EC) cells were scattered throughout the duct system and were enriched in islets of Langerhans. The fluid secretory rate in the isolated interlobular ducts was measured by videomicroscopy. Basolateral applications of 5-HT strongly but reversibly reduced HCO3-dependent, as well as secretin- and acetylcholine- (ACh-) stimulated, fluid secretion, whereas 5-HT applied into the lumen had no such effects. Secretin-stimulated fluid secretion could be inhibited by a 5-HT3 receptor agonist, but not by agonists of the 5-HT1, 5-HT2, or 5-HT4 receptors. Under the stimulation with secretin, 5-HT decreased the intracellular pH (pHi) and reduced the rate of pHi recovery after acid loading with NH4+, suggesting that 5-HT inhibits the intracellular accumulation of HCO3-. The elevation of intraductal pressure in vivo reduced secretin-stimulated fluid secretion, an effect that could be attenuated by a 5-HT3 receptor antagonist. Thus, 5-HT, acting through basolateral 5-HT3 receptors, strongly inhibits spontaneous, secretin-, and ACh-stimulated fluid secretion by guinea pig pancreatic ducts. 5-HT released from pancreatic ductal EC cells on elevation of the intraductal pressure may regulate fluid secretion of neighboring duct cells in a paracrine fashion.

AB - We studied the distribution of 5-hydroxytryptamine- (5-HT-) containing cells in the guinea pig pancreas and examined the effects of 5-HT on fluid secretion by interlobular pancreatic ducts. The 5-HT-immunoreactive cells with morphological characteristics of enterochromaffin (EC) cells were scattered throughout the duct system and were enriched in islets of Langerhans. The fluid secretory rate in the isolated interlobular ducts was measured by videomicroscopy. Basolateral applications of 5-HT strongly but reversibly reduced HCO3-dependent, as well as secretin- and acetylcholine- (ACh-) stimulated, fluid secretion, whereas 5-HT applied into the lumen had no such effects. Secretin-stimulated fluid secretion could be inhibited by a 5-HT3 receptor agonist, but not by agonists of the 5-HT1, 5-HT2, or 5-HT4 receptors. Under the stimulation with secretin, 5-HT decreased the intracellular pH (pHi) and reduced the rate of pHi recovery after acid loading with NH4+, suggesting that 5-HT inhibits the intracellular accumulation of HCO3-. The elevation of intraductal pressure in vivo reduced secretin-stimulated fluid secretion, an effect that could be attenuated by a 5-HT3 receptor antagonist. Thus, 5-HT, acting through basolateral 5-HT3 receptors, strongly inhibits spontaneous, secretin-, and ACh-stimulated fluid secretion by guinea pig pancreatic ducts. 5-HT released from pancreatic ductal EC cells on elevation of the intraductal pressure may regulate fluid secretion of neighboring duct cells in a paracrine fashion.

UR - http://www.scopus.com/inward/record.url?scp=0034794969&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034794969&partnerID=8YFLogxK

U2 - 10.1172/JCI200112312

DO - 10.1172/JCI200112312

M3 - Article

C2 - 11544281

AN - SCOPUS:0034794969

VL - 108

SP - 749

EP - 756

JO - Journal of Clinical Investigation

JF - Journal of Clinical Investigation

SN - 0021-9738

IS - 5

ER -