5-Lipoxygenase inhibitor (AA-861) attenuates neutrophil-mediated oxidative stress on the venular endothelium in endotoxemia

M. Suematsu, S. Miura, M. Suzuki, H. Nagata, T. Morishita, C. Oshio, M. Tsuchiya

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Neutrophil-mediated oxidative stress on the rat mesenteric microcirculation was studied in the experimental model of endotoxin-induced disseminated intravascular coagulation (DIC) by using an intravital fluorescent microscope equipped with a Silicon Intensifier Target Image Tube camera and luminol-dependent chemiluminescence (ChL) analysis. Leukocytes adhering to the venules were visualized by the injection of acridine orange, a fluorochrome tracer which shows high affinity to white cells. Endotoxin (E. coli, O-111 B4) was administered intravenously at a dose of 2 mg/kg/hour. After starting the infusion of endotoxin, the number of adherent cells gradually increased in the venular endothelium and was followed by a transient neutropenia. ChL activities from neutrophils were also significantly elevated, which may reflect the enhanced ability to generate oxygen-radicals. To elucidate the role of 5-lipoxygenase products in the locomotive and metabolic changes of neutrophils, the effects of AA-861, a specific inhibitor of 5-lipoxygenase was tested. In addition prednisolone and indomethacin were evaluated. AA-861 and prednisolone reduced neutropenia, leukocyte adhesion to the venular walls and ChL activities from neutrophils. It was concluded that 5-lipoxygenase may modulate neutrophil-mediated oxidative stress on microvasculature in endotoxin-induced DIC.

Original languageEnglish
Pages (from-to)41-45
Number of pages5
JournalJournal of Clinical and Laboratory Immunology
Volume25
Issue number1
Publication statusPublished - 1988

Fingerprint

Lipoxygenase Inhibitors
Endotoxemia
Endothelium
Oxidative Stress
Neutrophils
Endotoxins
Luminescence
Arachidonate 5-Lipoxygenase
Disseminated Intravascular Coagulation
Prednisolone
Neutropenia
Leukocytes
Luminol
Acridine Orange
Venules
Silicon
Microcirculation
Microvessels
Fluorescent Dyes
Indomethacin

ASJC Scopus subject areas

  • Immunology

Cite this

Suematsu, M., Miura, S., Suzuki, M., Nagata, H., Morishita, T., Oshio, C., & Tsuchiya, M. (1988). 5-Lipoxygenase inhibitor (AA-861) attenuates neutrophil-mediated oxidative stress on the venular endothelium in endotoxemia. Journal of Clinical and Laboratory Immunology, 25(1), 41-45.

5-Lipoxygenase inhibitor (AA-861) attenuates neutrophil-mediated oxidative stress on the venular endothelium in endotoxemia. / Suematsu, M.; Miura, S.; Suzuki, M.; Nagata, H.; Morishita, T.; Oshio, C.; Tsuchiya, M.

In: Journal of Clinical and Laboratory Immunology, Vol. 25, No. 1, 1988, p. 41-45.

Research output: Contribution to journalArticle

Suematsu, M, Miura, S, Suzuki, M, Nagata, H, Morishita, T, Oshio, C & Tsuchiya, M 1988, '5-Lipoxygenase inhibitor (AA-861) attenuates neutrophil-mediated oxidative stress on the venular endothelium in endotoxemia', Journal of Clinical and Laboratory Immunology, vol. 25, no. 1, pp. 41-45.
Suematsu, M. ; Miura, S. ; Suzuki, M. ; Nagata, H. ; Morishita, T. ; Oshio, C. ; Tsuchiya, M. / 5-Lipoxygenase inhibitor (AA-861) attenuates neutrophil-mediated oxidative stress on the venular endothelium in endotoxemia. In: Journal of Clinical and Laboratory Immunology. 1988 ; Vol. 25, No. 1. pp. 41-45.
@article{e9079682be1e445d8d9d86c80a2e3264,
title = "5-Lipoxygenase inhibitor (AA-861) attenuates neutrophil-mediated oxidative stress on the venular endothelium in endotoxemia",
abstract = "Neutrophil-mediated oxidative stress on the rat mesenteric microcirculation was studied in the experimental model of endotoxin-induced disseminated intravascular coagulation (DIC) by using an intravital fluorescent microscope equipped with a Silicon Intensifier Target Image Tube camera and luminol-dependent chemiluminescence (ChL) analysis. Leukocytes adhering to the venules were visualized by the injection of acridine orange, a fluorochrome tracer which shows high affinity to white cells. Endotoxin (E. coli, O-111 B4) was administered intravenously at a dose of 2 mg/kg/hour. After starting the infusion of endotoxin, the number of adherent cells gradually increased in the venular endothelium and was followed by a transient neutropenia. ChL activities from neutrophils were also significantly elevated, which may reflect the enhanced ability to generate oxygen-radicals. To elucidate the role of 5-lipoxygenase products in the locomotive and metabolic changes of neutrophils, the effects of AA-861, a specific inhibitor of 5-lipoxygenase was tested. In addition prednisolone and indomethacin were evaluated. AA-861 and prednisolone reduced neutropenia, leukocyte adhesion to the venular walls and ChL activities from neutrophils. It was concluded that 5-lipoxygenase may modulate neutrophil-mediated oxidative stress on microvasculature in endotoxin-induced DIC.",
author = "M. Suematsu and S. Miura and M. Suzuki and H. Nagata and T. Morishita and C. Oshio and M. Tsuchiya",
year = "1988",
language = "English",
volume = "25",
pages = "41--45",
journal = "Journal of Clinical and Laboratory Immunology",
issn = "0141-2760",
publisher = "Teviot Scientific Publications Ltd.",
number = "1",

}

TY - JOUR

T1 - 5-Lipoxygenase inhibitor (AA-861) attenuates neutrophil-mediated oxidative stress on the venular endothelium in endotoxemia

AU - Suematsu, M.

AU - Miura, S.

AU - Suzuki, M.

AU - Nagata, H.

AU - Morishita, T.

AU - Oshio, C.

AU - Tsuchiya, M.

PY - 1988

Y1 - 1988

N2 - Neutrophil-mediated oxidative stress on the rat mesenteric microcirculation was studied in the experimental model of endotoxin-induced disseminated intravascular coagulation (DIC) by using an intravital fluorescent microscope equipped with a Silicon Intensifier Target Image Tube camera and luminol-dependent chemiluminescence (ChL) analysis. Leukocytes adhering to the venules were visualized by the injection of acridine orange, a fluorochrome tracer which shows high affinity to white cells. Endotoxin (E. coli, O-111 B4) was administered intravenously at a dose of 2 mg/kg/hour. After starting the infusion of endotoxin, the number of adherent cells gradually increased in the venular endothelium and was followed by a transient neutropenia. ChL activities from neutrophils were also significantly elevated, which may reflect the enhanced ability to generate oxygen-radicals. To elucidate the role of 5-lipoxygenase products in the locomotive and metabolic changes of neutrophils, the effects of AA-861, a specific inhibitor of 5-lipoxygenase was tested. In addition prednisolone and indomethacin were evaluated. AA-861 and prednisolone reduced neutropenia, leukocyte adhesion to the venular walls and ChL activities from neutrophils. It was concluded that 5-lipoxygenase may modulate neutrophil-mediated oxidative stress on microvasculature in endotoxin-induced DIC.

AB - Neutrophil-mediated oxidative stress on the rat mesenteric microcirculation was studied in the experimental model of endotoxin-induced disseminated intravascular coagulation (DIC) by using an intravital fluorescent microscope equipped with a Silicon Intensifier Target Image Tube camera and luminol-dependent chemiluminescence (ChL) analysis. Leukocytes adhering to the venules were visualized by the injection of acridine orange, a fluorochrome tracer which shows high affinity to white cells. Endotoxin (E. coli, O-111 B4) was administered intravenously at a dose of 2 mg/kg/hour. After starting the infusion of endotoxin, the number of adherent cells gradually increased in the venular endothelium and was followed by a transient neutropenia. ChL activities from neutrophils were also significantly elevated, which may reflect the enhanced ability to generate oxygen-radicals. To elucidate the role of 5-lipoxygenase products in the locomotive and metabolic changes of neutrophils, the effects of AA-861, a specific inhibitor of 5-lipoxygenase was tested. In addition prednisolone and indomethacin were evaluated. AA-861 and prednisolone reduced neutropenia, leukocyte adhesion to the venular walls and ChL activities from neutrophils. It was concluded that 5-lipoxygenase may modulate neutrophil-mediated oxidative stress on microvasculature in endotoxin-induced DIC.

UR - http://www.scopus.com/inward/record.url?scp=0023872315&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023872315&partnerID=8YFLogxK

M3 - Article

C2 - 3385768

AN - SCOPUS:0023872315

VL - 25

SP - 41

EP - 45

JO - Journal of Clinical and Laboratory Immunology

JF - Journal of Clinical and Laboratory Immunology

SN - 0141-2760

IS - 1

ER -