6'-sulfo sialyl Le(x) but not 6-sulfo sialyl Le(x) expressed on the cell surface supports L-selectin-mediated adhesion

Shigeru Tsuboi, Yukihiro Isogai, Noriyasu Hada, Jennifer K. King, Ole Hindsgaul, Minoru Fukuda

Research output: Contribution to journalArticle

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Abstract

In order to determine if a sulfated oligosaccharide on the cell surface can function as an L-selectin ligand, a novel approach for in vitro transfer of oligosaccharides was utilized (Srivastava, G., Kaun, K. J., Hindsgaul, O., and Palcic, M. M. (1992) J. Biol. Chem. 267, 22356-22361). CHO cells were incubated with synthetic 6'-sulfo sialyl Le(x), NeuNAcα2→3(sulfate- 6)Galβ1→4(Fucα1→3)GlcNAc or 6-sulfo sialyl Le(x), NeuNAcα2→3Galβ1→4[(Fucα1→3)sulfate→6GlcNAc] oligosaccharide linked to C-6 of a fucose residue in GDP-fucose and a milk fucosyltransferase. The resultant CHO cells expressing 6'-sulfo sialyl Le(x) or 6-sulfo sialyl Le(x) on their cell surface were tested for adhesion to E-selectin and L-selectin chimeric proteins coated on plates. The results indicate that 6'-sulfo sialyl Le(x) supports L-selectin-mediated adhesion much better than sialyl Le(x) similarly tagged on the cell surface. In contrast, 6-sulfo sialyl Le(x) containing a sulfate group on the N-acetylglucosamine residue did not support adhesion with either selectin. These combined results suggest that 6'-sulfo sialyl Le(x) is a much better ligand than sialyl Le(x) oligosaccharide for L- selectin.

Original languageEnglish
Pages (from-to)27213-27216
Number of pages4
JournalJournal of Biological Chemistry
Volume271
Issue number44
DOIs
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

L-Selectin
Adhesion
Oligosaccharides
CHO Cells
Sulfates
Guanosine Diphosphate Fucose
Fucosyltransferases
Ligands
5-acetylneuraminyl-(2-3)-galactosyl-(1-4)-(fucopyranosyl-(1-3))-N-acetylglucosamine
Selectins
E-Selectin
Fucose
Acetylglucosamine
Milk

ASJC Scopus subject areas

  • Biochemistry

Cite this

6'-sulfo sialyl Le(x) but not 6-sulfo sialyl Le(x) expressed on the cell surface supports L-selectin-mediated adhesion. / Tsuboi, Shigeru; Isogai, Yukihiro; Hada, Noriyasu; King, Jennifer K.; Hindsgaul, Ole; Fukuda, Minoru.

In: Journal of Biological Chemistry, Vol. 271, No. 44, 1996, p. 27213-27216.

Research output: Contribution to journalArticle

Tsuboi, Shigeru ; Isogai, Yukihiro ; Hada, Noriyasu ; King, Jennifer K. ; Hindsgaul, Ole ; Fukuda, Minoru. / 6'-sulfo sialyl Le(x) but not 6-sulfo sialyl Le(x) expressed on the cell surface supports L-selectin-mediated adhesion. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 44. pp. 27213-27216.
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T1 - 6'-sulfo sialyl Le(x) but not 6-sulfo sialyl Le(x) expressed on the cell surface supports L-selectin-mediated adhesion

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AU - Isogai, Yukihiro

AU - Hada, Noriyasu

AU - King, Jennifer K.

AU - Hindsgaul, Ole

AU - Fukuda, Minoru

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AB - In order to determine if a sulfated oligosaccharide on the cell surface can function as an L-selectin ligand, a novel approach for in vitro transfer of oligosaccharides was utilized (Srivastava, G., Kaun, K. J., Hindsgaul, O., and Palcic, M. M. (1992) J. Biol. Chem. 267, 22356-22361). CHO cells were incubated with synthetic 6'-sulfo sialyl Le(x), NeuNAcα2→3(sulfate- 6)Galβ1→4(Fucα1→3)GlcNAc or 6-sulfo sialyl Le(x), NeuNAcα2→3Galβ1→4[(Fucα1→3)sulfate→6GlcNAc] oligosaccharide linked to C-6 of a fucose residue in GDP-fucose and a milk fucosyltransferase. The resultant CHO cells expressing 6'-sulfo sialyl Le(x) or 6-sulfo sialyl Le(x) on their cell surface were tested for adhesion to E-selectin and L-selectin chimeric proteins coated on plates. The results indicate that 6'-sulfo sialyl Le(x) supports L-selectin-mediated adhesion much better than sialyl Le(x) similarly tagged on the cell surface. In contrast, 6-sulfo sialyl Le(x) containing a sulfate group on the N-acetylglucosamine residue did not support adhesion with either selectin. These combined results suggest that 6'-sulfo sialyl Le(x) is a much better ligand than sialyl Le(x) oligosaccharide for L- selectin.

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