A carbohydrate-binding protein, Galectin-1, promotes proliferation of adult neural stem cells

Masanori Sakaguchi, Tetsuro Shingo, Takuya Shimazaki, Hirotaka James Okano, Mieko Shiwa, Satoru Ishibashi, Hideyuki Oguro, Mikiko Ninomiya, Toshihiko Kadoya, Hidenori Horie, Akira Shibuya, Hidehiro Mizusawa, Françoise Poirier, Hiromitsu Nakauchi, Kazunobu Sawamoto, Hideyuki Okano

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

In the subventricular zone of the adult mammalian forebrain, neural stem cells (NSCs) reside and proliferate to generate young neurons. We screened factors that promoted the proliferation of NSCs in vitro by a recently developed proteomics technique, the ProteinChip system. In this screen, we identified a soluble carbohydrate-binding protein, Galectin-1, as a candidate. We show herein that Galectin-1 is expressed in a subset of slowly dividing subventricular zone astrocytes, which includes the NSCs. Based on results from intraventricular infusion experiments and phenotypic analyses of knockout mice, we demonstrate that Galectin-1 is an endogenous factor that promotes the proliferation of NSCs in the adult brain.

Original languageEnglish
Pages (from-to)7112-7117
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number18
DOIs
Publication statusPublished - 2006 May 2

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Galectin 1
Adult Stem Cells
Neural Stem Cells
Lateral Ventricles
Intraventricular Infusions
Protein Array Analysis
Prosencephalon
Knockout Mice
Astrocytes
Proteomics
Neurons
saccharide-binding proteins
Brain

Keywords

  • Lectin
  • Mobilization
  • Stem cell niche

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

A carbohydrate-binding protein, Galectin-1, promotes proliferation of adult neural stem cells. / Sakaguchi, Masanori; Shingo, Tetsuro; Shimazaki, Takuya; Okano, Hirotaka James; Shiwa, Mieko; Ishibashi, Satoru; Oguro, Hideyuki; Ninomiya, Mikiko; Kadoya, Toshihiko; Horie, Hidenori; Shibuya, Akira; Mizusawa, Hidehiro; Poirier, Françoise; Nakauchi, Hiromitsu; Sawamoto, Kazunobu; Okano, Hideyuki.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, No. 18, 02.05.2006, p. 7112-7117.

Research output: Contribution to journalArticle

Sakaguchi, M, Shingo, T, Shimazaki, T, Okano, HJ, Shiwa, M, Ishibashi, S, Oguro, H, Ninomiya, M, Kadoya, T, Horie, H, Shibuya, A, Mizusawa, H, Poirier, F, Nakauchi, H, Sawamoto, K & Okano, H 2006, 'A carbohydrate-binding protein, Galectin-1, promotes proliferation of adult neural stem cells', Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 18, pp. 7112-7117. https://doi.org/10.1073/pnas.0508793103
Sakaguchi, Masanori ; Shingo, Tetsuro ; Shimazaki, Takuya ; Okano, Hirotaka James ; Shiwa, Mieko ; Ishibashi, Satoru ; Oguro, Hideyuki ; Ninomiya, Mikiko ; Kadoya, Toshihiko ; Horie, Hidenori ; Shibuya, Akira ; Mizusawa, Hidehiro ; Poirier, Françoise ; Nakauchi, Hiromitsu ; Sawamoto, Kazunobu ; Okano, Hideyuki. / A carbohydrate-binding protein, Galectin-1, promotes proliferation of adult neural stem cells. In: Proceedings of the National Academy of Sciences of the United States of America. 2006 ; Vol. 103, No. 18. pp. 7112-7117.
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