A carrier-mediated transport system for benzylpenicillin in isolated hepatocytes

A. Tsuji, T. Terasaki, I. Tamai, E. Nakashima, K. Takanosu

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The transport mechanism of benzylpenicillin was studied in freshly prepared rat hepatocytes. The initial uptake rate followed both saturable and unsaturable transport processes. The Arrhenius plot of the initial uptake rate gave an activation energy of 16.8 kcal mol-1 (69 kJ mol-1). The benzylpenicillin uptake by hepatocyes was significantly inhibited by antimycin A, sodium cyanide, rotenone, 2,4-dinitrophenol, phenoxymethylpenicillin, probenecid and taurocholic acid. No significant inhibition was observed by acetylaminohippuric acid and several kinds of amino acids and dipeptides. The present study provides the first evidence for the existence of a carrier-mediated and energy-dependent transport system of benzylpenicillin in the liver.

Original languageEnglish
Pages (from-to)55-57
Number of pages3
JournalJournal of Pharmacy and Pharmacology
Volume37
Issue number1
Publication statusPublished - 1985
Externally publishedYes

Fingerprint

Penicillin G
Hepatocytes
Sodium Cyanide
Penicillin V
Antimycin A
2,4-Dinitrophenol
Probenecid
Rotenone
Taurocholic Acid
Dipeptides
Amino Acids
Acids
Liver

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

Cite this

Tsuji, A., Terasaki, T., Tamai, I., Nakashima, E., & Takanosu, K. (1985). A carrier-mediated transport system for benzylpenicillin in isolated hepatocytes. Journal of Pharmacy and Pharmacology, 37(1), 55-57.

A carrier-mediated transport system for benzylpenicillin in isolated hepatocytes. / Tsuji, A.; Terasaki, T.; Tamai, I.; Nakashima, E.; Takanosu, K.

In: Journal of Pharmacy and Pharmacology, Vol. 37, No. 1, 1985, p. 55-57.

Research output: Contribution to journalArticle

Tsuji, A, Terasaki, T, Tamai, I, Nakashima, E & Takanosu, K 1985, 'A carrier-mediated transport system for benzylpenicillin in isolated hepatocytes', Journal of Pharmacy and Pharmacology, vol. 37, no. 1, pp. 55-57.
Tsuji, A. ; Terasaki, T. ; Tamai, I. ; Nakashima, E. ; Takanosu, K. / A carrier-mediated transport system for benzylpenicillin in isolated hepatocytes. In: Journal of Pharmacy and Pharmacology. 1985 ; Vol. 37, No. 1. pp. 55-57.
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