TY - JOUR
T1 - A carrier‐mediated transport system for benzylpenicillin in isolated hepatocytes
AU - Tsuji, Akir
AU - Terasaki, Tetsuya
AU - Tamai, Ikumi
AU - Nakashima, Emi
AU - Takanosu, Kazuhiro
PY - 1985/1
Y1 - 1985/1
N2 - The transport mechanism of benzylpenicillin was studied in freshly prepared rat hepatocytes. The initial uptake rate followed both saturable and unsaturable transport processes. The Arrhenius plot of the initial uptake rate gave an activation energy of 16.8 kcal mol−1 (69 kJ mol−1). The benzylpenicillin uptake by hepatocytes was significantly inhibited by antimycin A, sodium cyanide, rotenone, 2,4‐dinitrophenol, phenoxymethylpenicillin, probenecid and Taurocholic acid. No significant inhibition was observed by acetylaminohippuric acid and several kinds of amino acids and dipeptides. The present study provides the first evidence for the the existence of a carrier‐mediated and energy‐dependent transport system of benzylpenicillin in the liver. 1985 Royal Pharmaceutical Society of Great Britain
AB - The transport mechanism of benzylpenicillin was studied in freshly prepared rat hepatocytes. The initial uptake rate followed both saturable and unsaturable transport processes. The Arrhenius plot of the initial uptake rate gave an activation energy of 16.8 kcal mol−1 (69 kJ mol−1). The benzylpenicillin uptake by hepatocytes was significantly inhibited by antimycin A, sodium cyanide, rotenone, 2,4‐dinitrophenol, phenoxymethylpenicillin, probenecid and Taurocholic acid. No significant inhibition was observed by acetylaminohippuric acid and several kinds of amino acids and dipeptides. The present study provides the first evidence for the the existence of a carrier‐mediated and energy‐dependent transport system of benzylpenicillin in the liver. 1985 Royal Pharmaceutical Society of Great Britain
UR - http://www.scopus.com/inward/record.url?scp=0021925488&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021925488&partnerID=8YFLogxK
U2 - 10.1111/j.2042-7158.1985.tb04931.x
DO - 10.1111/j.2042-7158.1985.tb04931.x
M3 - Article
C2 - 2858529
AN - SCOPUS:0021925488
SN - 0022-3573
VL - 37
SP - 55
EP - 57
JO - Journal of Pharmacy and Pharmacology
JF - Journal of Pharmacy and Pharmacology
IS - 1
ER -