A cellular protein which is coprecipitated with HTLV-I rex protein in the presence of the target mRNA

Jun Katahira, Haruhiko Siomi, Toshimasa Ishizaki, Tomoe Umemoto, Yuetsu Tanaka, Hisatoshi Shida

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We examined the cellular protein(s) which can associate with Rex protein of human T cell leukemia virus type I (HTLV-I), using Rex-maltose binding protein (MBP) fusion protein. Immunoprecipitation of RexMBP with anti-MBP antibody revealed that a 24 kD protein (p24) associated with RexMBP only in the presence of Rex-responsive mRNA, The fact that p24 was present in both the nucleus and the cytoplasm is consistent with a role of Rex in the nucleo-cytoplasmic transport of viral mRNAs. P24 did not interact with nonfunctional Rex mutant proteins even if they had RNA binding activity in vitro. These results suggest the possible involvement of p24 in the Rex function through a complex formation with Rex on Rex-responsive mRNA.

Original languageEnglish
Pages (from-to)3535-3544
Number of pages10
JournalOncogene
Volume9
Issue number12
Publication statusPublished - 1994 Dec
Externally publishedYes

Fingerprint

Maltose-Binding Proteins
Messenger RNA
rex Gene Products
Proteins
Cell Nucleus Active Transport
Mutant Proteins
Immunoprecipitation
Cytoplasm
RNA
Antibodies
Human T-lymphotropic virus 1 rex Protein
In Vitro Techniques

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Katahira, J., Siomi, H., Ishizaki, T., Umemoto, T., Tanaka, Y., & Shida, H. (1994). A cellular protein which is coprecipitated with HTLV-I rex protein in the presence of the target mRNA. Oncogene, 9(12), 3535-3544.

A cellular protein which is coprecipitated with HTLV-I rex protein in the presence of the target mRNA. / Katahira, Jun; Siomi, Haruhiko; Ishizaki, Toshimasa; Umemoto, Tomoe; Tanaka, Yuetsu; Shida, Hisatoshi.

In: Oncogene, Vol. 9, No. 12, 12.1994, p. 3535-3544.

Research output: Contribution to journalArticle

Katahira, J, Siomi, H, Ishizaki, T, Umemoto, T, Tanaka, Y & Shida, H 1994, 'A cellular protein which is coprecipitated with HTLV-I rex protein in the presence of the target mRNA', Oncogene, vol. 9, no. 12, pp. 3535-3544.
Katahira J, Siomi H, Ishizaki T, Umemoto T, Tanaka Y, Shida H. A cellular protein which is coprecipitated with HTLV-I rex protein in the presence of the target mRNA. Oncogene. 1994 Dec;9(12):3535-3544.
Katahira, Jun ; Siomi, Haruhiko ; Ishizaki, Toshimasa ; Umemoto, Tomoe ; Tanaka, Yuetsu ; Shida, Hisatoshi. / A cellular protein which is coprecipitated with HTLV-I rex protein in the presence of the target mRNA. In: Oncogene. 1994 ; Vol. 9, No. 12. pp. 3535-3544.
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