A common cis-acting sequence in the DiGeorge critical region regulates bi-directional transcription of UFD1L and CDC45L

Amit Kunte, Kathryn Ivey, Chihiro Yamagishi, Vidu Garg, Hiroyuki Yamagishi, Deepak Srivastava

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Two to three megabase deletions on chromosome 22q11 are the cytogenetic findings most commonly associated with cardiac and craniofacial defects in humans. The constellation of clinical findings associated with these deletions is termed the 22q11 deletion syndrome. We had earlier described a patient with the 22q11 deletion phenotype who was hemizygous for an atypical 20 kb microdeletion in this region. The deletion included coding regions of two genes organized head-to-head, UFD1L and CDC45L, along with an 884 bp CpG-rich intervening region. Based on this genomic organization, we hypothesized that both genes may be co-expressed and co-regulated by sequences within this region. We demonstrate that expression of both genes is enhanced in a similar pattern in precursors of structures affected by the deletion. The intergenic region is sufficient to direct transcription most strongly in the developing pharyngeal arches and limb buds of transgenic mice and can also direct bi-directional transcriptional activation in a neural crest-derived cell line. Deletion analyses revealed that a 404 bp fragment closest to UFD1L is necessary and sufficient to direct this bi-directional transcriptional activity. These results reveal the presence of a conserved regulatory region in the 22q11 deletion locus that can direct simultaneous transcription of genes involved in ubiquitin mediated protein processing (UFD1L) and cell cycle control (CDC45L).

Original languageEnglish
Pages (from-to)81-92
Number of pages12
JournalMechanisms of Development
Volume108
Issue number1-2
DOIs
Publication statusPublished - 2001
Externally publishedYes

Keywords

  • 22q11
  • Bi-directional promoters
  • CDC45L
  • CpG islands
  • DiGeorge
  • Neural crest
  • UFD1L

ASJC Scopus subject areas

  • Embryology
  • Developmental Biology

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