A comparison of chromosome repair kinetics in G0 and G1 reveals that enhanced repair fidelity under noncycling conditions accounts for increased potentially lethal damage repair

Cuihua Liu, Tetsuya Kawata, Naoyuki Shigematsu, Francis Cucinotta, Kerry George, Masayoshi Saito, Takashi Uno, Kouichi Isobe, Hisao Ito

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Potentially lethal damage (PLD) and its repair were studied in confluent human fibroblasts by analyzing the kinetics of chromosome break rejoining and misrejoining in irradiated cells that were either held in noncycling G0 phase or allowed to enter G1 phase of the cell cycle immediately after 6 Gy irradiation. Virally mediated premature chromosome condensation (PCC) methods were combined with fluorescence in situ hybridization (FISH) to study chromosomal aberrations in interphase. Flow cytometry revealed that the vast majority of cells had not yet entered S phase 15 h after release from G0. By this time some 95 of initially produced prematurely condensed chromosome breaks had rejoined, indicating that most repair processes occurred during G1. The rejoining kinetics of prematurely condensed chromosome breaks was similar for each culture condition. However, under noncycling conditions misrepair peaked at 0.55 exchanges per cell, while under cycling conditions (G1) it peaked at 1.1 exchanges per cell. At 12 h postirradiation, complex-type exchanges were sevenfold more abundant for cycling cells (G1) than for noncycling cells (G0). Since most repair in G0/G1 occurs via the non-homologous end-joining (NHEJ) process, increased PLD repair may result from improved cell cycle-specific rejoining fidelity of the NHEJ pathway.

Original languageEnglish
Pages (from-to)566-573
Number of pages8
JournalRadiation Research
Volume174
Issue number5
DOIs
Publication statusPublished - 2010 Nov

Fingerprint

chromosomes
Chromosomes
damage
Chromosome Breakage
kinetics
cells
cycles
Cell Cycle
cytometry
fibroblasts
Cell Cycle Resting Phase
Interphase
G1 Phase
aberration
Fluorescence In Situ Hybridization
S Phase
Chromosome Aberrations
condensation
Flow Cytometry
Fibroblasts

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Biophysics
  • Radiation

Cite this

A comparison of chromosome repair kinetics in G0 and G1 reveals that enhanced repair fidelity under noncycling conditions accounts for increased potentially lethal damage repair. / Liu, Cuihua; Kawata, Tetsuya; Shigematsu, Naoyuki; Cucinotta, Francis; George, Kerry; Saito, Masayoshi; Uno, Takashi; Isobe, Kouichi; Ito, Hisao.

In: Radiation Research, Vol. 174, No. 5, 11.2010, p. 566-573.

Research output: Contribution to journalArticle

Liu, Cuihua ; Kawata, Tetsuya ; Shigematsu, Naoyuki ; Cucinotta, Francis ; George, Kerry ; Saito, Masayoshi ; Uno, Takashi ; Isobe, Kouichi ; Ito, Hisao. / A comparison of chromosome repair kinetics in G0 and G1 reveals that enhanced repair fidelity under noncycling conditions accounts for increased potentially lethal damage repair. In: Radiation Research. 2010 ; Vol. 174, No. 5. pp. 566-573.
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