A continuous-flow microbial microreactor using microbes immobilized into a microporous carrier by dielectrophoresis

T. Kano, T. Inaba, Y. Gu, Norihisa Miki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

In this paper we demonstrate a continuous-flow microbial microreactor that immobilizes microbes into microporous carrier. First, bacteria are trapped into 3, 5, 10, 20-μm-diameter pores using positive dielectrophoresis (DEP). After non-immobilized bacteria are flushed, the continuous-flow microreactor generates reaction products when culture media including reactive substrate are supplied. Continuous-flow type reactors facilitate collecting and evaluating reaction products. We used the developed microreactor to characterize microbes belonging to Corynebacterium group by measuring generated lactic acid when glucose was supplied. The amount of lactic acid produced by a single bacterium was deduced and we found Corynebacterium variabile to be most productive among three tested members. The microbial reactor proposed herein is readily applicable to an efficient microbial screening platform.

Original languageEnglish
Title of host publicationProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Pages745-748
Number of pages4
DOIs
Publication statusPublished - 2012
Event2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 - Paris, France
Duration: 2012 Jan 292012 Feb 2

Other

Other2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
CountryFrance
CityParis
Period12/1/2912/2/2

Fingerprint

microorganisms
Electrophoresis
bacteria
Bacteria
lactic acid
Lactic acid
Reaction products
reaction products
Lactic Acid
reactors
culture media
glucose
Glucose
Culture Media
Screening
screening
platforms
porosity
Substrates

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

Kano, T., Inaba, T., Gu, Y., & Miki, N. (2012). A continuous-flow microbial microreactor using microbes immobilized into a microporous carrier by dielectrophoresis. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) (pp. 745-748). [6170293] https://doi.org/10.1109/MEMSYS.2012.6170293

A continuous-flow microbial microreactor using microbes immobilized into a microporous carrier by dielectrophoresis. / Kano, T.; Inaba, T.; Gu, Y.; Miki, Norihisa.

Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2012. p. 745-748 6170293.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kano, T, Inaba, T, Gu, Y & Miki, N 2012, A continuous-flow microbial microreactor using microbes immobilized into a microporous carrier by dielectrophoresis. in Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)., 6170293, pp. 745-748, 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012, Paris, France, 12/1/29. https://doi.org/10.1109/MEMSYS.2012.6170293
Kano T, Inaba T, Gu Y, Miki N. A continuous-flow microbial microreactor using microbes immobilized into a microporous carrier by dielectrophoresis. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2012. p. 745-748. 6170293 https://doi.org/10.1109/MEMSYS.2012.6170293
Kano, T. ; Inaba, T. ; Gu, Y. ; Miki, Norihisa. / A continuous-flow microbial microreactor using microbes immobilized into a microporous carrier by dielectrophoresis. Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2012. pp. 745-748
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