TY - GEN
T1 - A continuous-flow microbial microreactor using microbes immobilized into a microporous carrier by dielectrophoresis
AU - Kano, T.
AU - Inaba, T.
AU - Gu, Y.
AU - Miki, N.
PY - 2012
Y1 - 2012
N2 - In this paper we demonstrate a continuous-flow microbial microreactor that immobilizes microbes into microporous carrier. First, bacteria are trapped into 3, 5, 10, 20-μm-diameter pores using positive dielectrophoresis (DEP). After non-immobilized bacteria are flushed, the continuous-flow microreactor generates reaction products when culture media including reactive substrate are supplied. Continuous-flow type reactors facilitate collecting and evaluating reaction products. We used the developed microreactor to characterize microbes belonging to Corynebacterium group by measuring generated lactic acid when glucose was supplied. The amount of lactic acid produced by a single bacterium was deduced and we found Corynebacterium variabile to be most productive among three tested members. The microbial reactor proposed herein is readily applicable to an efficient microbial screening platform.
AB - In this paper we demonstrate a continuous-flow microbial microreactor that immobilizes microbes into microporous carrier. First, bacteria are trapped into 3, 5, 10, 20-μm-diameter pores using positive dielectrophoresis (DEP). After non-immobilized bacteria are flushed, the continuous-flow microreactor generates reaction products when culture media including reactive substrate are supplied. Continuous-flow type reactors facilitate collecting and evaluating reaction products. We used the developed microreactor to characterize microbes belonging to Corynebacterium group by measuring generated lactic acid when glucose was supplied. The amount of lactic acid produced by a single bacterium was deduced and we found Corynebacterium variabile to be most productive among three tested members. The microbial reactor proposed herein is readily applicable to an efficient microbial screening platform.
UR - http://www.scopus.com/inward/record.url?scp=84860427629&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84860427629&partnerID=8YFLogxK
U2 - 10.1109/MEMSYS.2012.6170293
DO - 10.1109/MEMSYS.2012.6170293
M3 - Conference contribution
AN - SCOPUS:84860427629
SN - 9781467303248
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 745
EP - 748
BT - 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
T2 - 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
Y2 - 29 January 2012 through 2 February 2012
ER -