A continuous-flow microbial microreactor using microbes immobilized into a microporous carrier by dielectrophoresis

T. Kano, T. Inaba, Y. Gu, N. Miki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

In this paper we demonstrate a continuous-flow microbial microreactor that immobilizes microbes into microporous carrier. First, bacteria are trapped into 3, 5, 10, 20-μm-diameter pores using positive dielectrophoresis (DEP). After non-immobilized bacteria are flushed, the continuous-flow microreactor generates reaction products when culture media including reactive substrate are supplied. Continuous-flow type reactors facilitate collecting and evaluating reaction products. We used the developed microreactor to characterize microbes belonging to Corynebacterium group by measuring generated lactic acid when glucose was supplied. The amount of lactic acid produced by a single bacterium was deduced and we found Corynebacterium variabile to be most productive among three tested members. The microbial reactor proposed herein is readily applicable to an efficient microbial screening platform.

Original languageEnglish
Title of host publication2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
Pages745-748
Number of pages4
DOIs
Publication statusPublished - 2012 May 7
Event2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 - Paris, France
Duration: 2012 Jan 292012 Feb 2

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Other

Other2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
CountryFrance
CityParis
Period12/1/2912/2/2

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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  • Cite this

    Kano, T., Inaba, T., Gu, Y., & Miki, N. (2012). A continuous-flow microbial microreactor using microbes immobilized into a microporous carrier by dielectrophoresis. In 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 (pp. 745-748). [6170293] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2012.6170293