A direct role for Hsp90 in pre-RISC formation in Drosophila

Tomohiro Miyoshi; Akiko Takeuchi; Haruhiko Siomi; Mikiko C. Siomi

doi:10.1038/nsmb.1875

A direct role for Hsp90 in pre-RISC formation in Drosophila

Tomohiro Miyoshi, Akiko Takeuchi, Haruhiko Siomi, Mikiko C. Siomi

Department of Molecular Biology

Research output: Contribution to journal › Article › peer-review

118 Citations (Scopus)

Abstract

Heat-shock proteins (Hsps) are molecular chaperones that control protein folding and function. Argonaute 2 (Ago2), the effector in RNA interference (RNAi), is associated with Hsp90; however, its function in RNAi remains elusive. Here we show that Hsp90 is required for Ago2 to receive the small interfering RNA (siRNA) duplex from the RNA-induced silencing complexĝ€" loading complex in RNAi, suggesting a model where Hsp90 modifies Ago2 conformation to accommodate the siRNA duplex.

Original language	English
Pages (from-to)	1024-1026
Number of pages	3
Journal	Nature Structural and Molecular Biology
Volume	17
Issue number	8
DOIs	https://doi.org/10.1038/nsmb.1875
Publication status	Published - 2010 Aug

ASJC Scopus subject areas

Structural Biology
Molecular Biology

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10.1038/nsmb.1875

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title = "A direct role for Hsp90 in pre-RISC formation in Drosophila",

abstract = "Heat-shock proteins (Hsps) are molecular chaperones that control protein folding and function. Argonaute 2 (Ago2), the effector in RNA interference (RNAi), is associated with Hsp90; however, its function in RNAi remains elusive. Here we show that Hsp90 is required for Ago2 to receive the small interfering RNA (siRNA) duplex from the RNA-induced silencing complexĝ€{"} loading complex in RNAi, suggesting a model where Hsp90 modifies Ago2 conformation to accommodate the siRNA duplex.",

author = "Tomohiro Miyoshi and Akiko Takeuchi and Haruhiko Siomi and Siomi, {Mikiko C.}",

note = "Funding Information: We thank K. Miyoshi and T. Yoshihisa for providing technical advice and other members of the Siomi laboratory for discussions. This work was supported by Ministry of Education, Culture, Sports, Science and Technology of Japan grants to H.S. and M.C.S. M.C.S. is supported by Core Research for Evolutional Science and Technology from the Japan Science and Technology Agency.",

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AU - Miyoshi, Tomohiro

AU - Takeuchi, Akiko

AU - Siomi, Haruhiko

AU - Siomi, Mikiko C.

N1 - Funding Information: We thank K. Miyoshi and T. Yoshihisa for providing technical advice and other members of the Siomi laboratory for discussions. This work was supported by Ministry of Education, Culture, Sports, Science and Technology of Japan grants to H.S. and M.C.S. M.C.S. is supported by Core Research for Evolutional Science and Technology from the Japan Science and Technology Agency.

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AB - Heat-shock proteins (Hsps) are molecular chaperones that control protein folding and function. Argonaute 2 (Ago2), the effector in RNA interference (RNAi), is associated with Hsp90; however, its function in RNAi remains elusive. Here we show that Hsp90 is required for Ago2 to receive the small interfering RNA (siRNA) duplex from the RNA-induced silencing complexĝ€" loading complex in RNAi, suggesting a model where Hsp90 modifies Ago2 conformation to accommodate the siRNA duplex.

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A direct role for Hsp90 in pre-RISC formation in Drosophila

Abstract

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