A fiber-optic measurement system of light scattering to evaluate embryo viability: model experiment using a latex sphere suspension and mouse embryos.

H. Itoh, Tsunenori Arai, M. Kikuchi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In this study, we tested the efficacy of a fiber-optic light scattering system developed by us to measure mitochondrial size and particle density in embryos in order to establish a non-invasive method of evaluating human embryo viability for in vitro fertilization and embryo transfer. Particle size was determined by comparing the measured angular distribution of the light scattering intensity with that of the theoretical distribution using a 1.0 micron latex sphere suspension and mouse embryos. The measured light scattering pattern of the latex suspension was found to agree closely with the theoretically derived pattern. The measured light scattering pattern of the mouse embryo was similar to the calculated pattern of spheres, the diameter of which was equivalent to that of the mitochondria. Therefore, the measured light scattering is thought to be derived mainly from the mitochondria in the embryo. The relationship between light scattering intensity and latex particle density was also examined in order to estimate the detectable range of mitochondrial particle density. Since the detectable range of the particle density obtained in our study (1.4 x 10(6) - 1.4 x 10(11) particles/ml) was greater than the known mitochondrial particle density in human embryos (1.0 x 10(9) - 1.0 x 10(11) particles/ml), we believe that this mitochondrial particle density can be measured using our system. We concluded that it is possible to use our simple system as a non-invasive method for evaluating human embryo viability.

Original languageEnglish
Pages (from-to)101-111
Number of pages11
JournalFrontiers of medical and biological engineering : the international journal of the Japan Society of Medical Electronics and Biological Engineering
Volume9
Issue number2
Publication statusPublished - 1999
Externally publishedYes

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Myelinated Nerve Fibers
Microspheres
Suspensions
Embryonic Structures
Light
Mitochondria
Mitochondrial Size
Embryo Transfer
Latex
Fertilization in Vitro
Particle Size

ASJC Scopus subject areas

  • Biophysics

Cite this

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abstract = "In this study, we tested the efficacy of a fiber-optic light scattering system developed by us to measure mitochondrial size and particle density in embryos in order to establish a non-invasive method of evaluating human embryo viability for in vitro fertilization and embryo transfer. Particle size was determined by comparing the measured angular distribution of the light scattering intensity with that of the theoretical distribution using a 1.0 micron latex sphere suspension and mouse embryos. The measured light scattering pattern of the latex suspension was found to agree closely with the theoretically derived pattern. The measured light scattering pattern of the mouse embryo was similar to the calculated pattern of spheres, the diameter of which was equivalent to that of the mitochondria. Therefore, the measured light scattering is thought to be derived mainly from the mitochondria in the embryo. The relationship between light scattering intensity and latex particle density was also examined in order to estimate the detectable range of mitochondrial particle density. Since the detectable range of the particle density obtained in our study (1.4 x 10(6) - 1.4 x 10(11) particles/ml) was greater than the known mitochondrial particle density in human embryos (1.0 x 10(9) - 1.0 x 10(11) particles/ml), we believe that this mitochondrial particle density can be measured using our system. We concluded that it is possible to use our simple system as a non-invasive method for evaluating human embryo viability.",
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