A functional single nucleotide polymorphism in mucin 1, at chromosome 1q22, determines susceptibility to diffuse-type gastric cancer

Norihisa Saeki, Akira Saito, Il Ju Choi, Keitaro Matsuo, Sumiko Ohnami, Hirohiko Totsuka, Suenori Chiku, Aya Kuchiba, Yeonsu Lee, Kyongah Yoon, Myeongcherl Kook, Sook Ryun Park, Youngwoo Kim, Hideo Tanaka, Kazuo Tajima, Hiroshi Hirose, Fumihiko Tanioka, Yoshihiro Matsuno, Haruhiko Sugimura, Shunji KatoTsuneya Nakamura, Tomohiro Nishina, Wataru Yasui, Kazuhiko Aoyagi, Hiroki Sasaki, Kazuyoshi Yanagihara, Hitoshi Katai, Tadakazu Shimoda, Teruhiko Yoshida, Yusuke Nakamura, Setsuo Hirohashi, Hiromi Sakamoto

Research output: Contribution to journalArticle

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Abstract

Background & Aims Two major types of gastric cancer, intestinal and diffuse, develop through distinct mechanisms; the diffuse type is considered to be more influenced by genetic factors, although the mechanism is unknown. Our previous genome-wide association study associated 3 single nucleotide polymorphisms (SNPs) with diffuse-type gastric cancer (DGC); 1 was a functional SNP (rs2294008) in prostate stem cell antigen (PSCA), but the loci of the other 2 were not investigated. Methods We performed high-density mapping to explore a linkage disequilibrium status of the 2 SNPs at chromosome 1q22. A DGC case-control study was conducted using DNA from 606 cases and 1264 controls (all Japanese individuals) and validated using DNA from Japanese (304 cases, 1465 controls) and Korean (452 cases, 372 controls) individuals. The effects of SNPs on function were analyzed by reporter assays and analyses of splice variants. Results A region of a strong linkage disequilibrium with the 2 SNPs contained mucin 1 (MUC1) and other 4 genes and SNPs significantly associated with DGC (rs2070803: P = 4.33 × 10-13; odds ratio [OR], 1.71 by meta-analysis of the studies on the 3 panels) but not with intestinal-type gastric cancer. Functional studies demonstrated that rs4072037 (P = 1.43 × 10-11; OR, 1.66 by meta-analysis) in MUC1 affects promoter activity and determines the major splicing variants of MUC1 in the gastric epithelium. Individuals that carry both SNPs rs2294008 in PSCA and rs4072037 in MUC1 have a high risk for developing DGC (OR, 8.38). Conclusions MUC1 is the second major DGC susceptibility gene identified. The SNPs rs2070803 and rs4072037 in MUC1 might be used to identify individuals at risk for this type of gastric cancer.

Original languageEnglish
Pages (from-to)892-902
Number of pages11
JournalGastroenterology
Volume140
Issue number3
DOIs
Publication statusPublished - 2011 Mar

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Mucin-1
Stomach Neoplasms
Single Nucleotide Polymorphism
Chromosomes
Odds Ratio
Linkage Disequilibrium
Meta-Analysis
Prostate
Stem Cells
Intestinal Neoplasms
Antigens
Neoplasm Genes
Genome-Wide Association Study
DNA
Case-Control Studies
Stomach
Epithelium

Keywords

  • Cancer Prevention
  • Genome-Wide Association Study
  • Risk Genotype
  • Stomach Cancer

ASJC Scopus subject areas

  • Gastroenterology

Cite this

A functional single nucleotide polymorphism in mucin 1, at chromosome 1q22, determines susceptibility to diffuse-type gastric cancer. / Saeki, Norihisa; Saito, Akira; Choi, Il Ju; Matsuo, Keitaro; Ohnami, Sumiko; Totsuka, Hirohiko; Chiku, Suenori; Kuchiba, Aya; Lee, Yeonsu; Yoon, Kyongah; Kook, Myeongcherl; Park, Sook Ryun; Kim, Youngwoo; Tanaka, Hideo; Tajima, Kazuo; Hirose, Hiroshi; Tanioka, Fumihiko; Matsuno, Yoshihiro; Sugimura, Haruhiko; Kato, Shunji; Nakamura, Tsuneya; Nishina, Tomohiro; Yasui, Wataru; Aoyagi, Kazuhiko; Sasaki, Hiroki; Yanagihara, Kazuyoshi; Katai, Hitoshi; Shimoda, Tadakazu; Yoshida, Teruhiko; Nakamura, Yusuke; Hirohashi, Setsuo; Sakamoto, Hiromi.

In: Gastroenterology, Vol. 140, No. 3, 03.2011, p. 892-902.

Research output: Contribution to journalArticle

Saeki, N, Saito, A, Choi, IJ, Matsuo, K, Ohnami, S, Totsuka, H, Chiku, S, Kuchiba, A, Lee, Y, Yoon, K, Kook, M, Park, SR, Kim, Y, Tanaka, H, Tajima, K, Hirose, H, Tanioka, F, Matsuno, Y, Sugimura, H, Kato, S, Nakamura, T, Nishina, T, Yasui, W, Aoyagi, K, Sasaki, H, Yanagihara, K, Katai, H, Shimoda, T, Yoshida, T, Nakamura, Y, Hirohashi, S & Sakamoto, H 2011, 'A functional single nucleotide polymorphism in mucin 1, at chromosome 1q22, determines susceptibility to diffuse-type gastric cancer', Gastroenterology, vol. 140, no. 3, pp. 892-902. https://doi.org/10.1053/j.gastro.2010.10.058
Saeki, Norihisa ; Saito, Akira ; Choi, Il Ju ; Matsuo, Keitaro ; Ohnami, Sumiko ; Totsuka, Hirohiko ; Chiku, Suenori ; Kuchiba, Aya ; Lee, Yeonsu ; Yoon, Kyongah ; Kook, Myeongcherl ; Park, Sook Ryun ; Kim, Youngwoo ; Tanaka, Hideo ; Tajima, Kazuo ; Hirose, Hiroshi ; Tanioka, Fumihiko ; Matsuno, Yoshihiro ; Sugimura, Haruhiko ; Kato, Shunji ; Nakamura, Tsuneya ; Nishina, Tomohiro ; Yasui, Wataru ; Aoyagi, Kazuhiko ; Sasaki, Hiroki ; Yanagihara, Kazuyoshi ; Katai, Hitoshi ; Shimoda, Tadakazu ; Yoshida, Teruhiko ; Nakamura, Yusuke ; Hirohashi, Setsuo ; Sakamoto, Hiromi. / A functional single nucleotide polymorphism in mucin 1, at chromosome 1q22, determines susceptibility to diffuse-type gastric cancer. In: Gastroenterology. 2011 ; Vol. 140, No. 3. pp. 892-902.
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abstract = "Background & Aims Two major types of gastric cancer, intestinal and diffuse, develop through distinct mechanisms; the diffuse type is considered to be more influenced by genetic factors, although the mechanism is unknown. Our previous genome-wide association study associated 3 single nucleotide polymorphisms (SNPs) with diffuse-type gastric cancer (DGC); 1 was a functional SNP (rs2294008) in prostate stem cell antigen (PSCA), but the loci of the other 2 were not investigated. Methods We performed high-density mapping to explore a linkage disequilibrium status of the 2 SNPs at chromosome 1q22. A DGC case-control study was conducted using DNA from 606 cases and 1264 controls (all Japanese individuals) and validated using DNA from Japanese (304 cases, 1465 controls) and Korean (452 cases, 372 controls) individuals. The effects of SNPs on function were analyzed by reporter assays and analyses of splice variants. Results A region of a strong linkage disequilibrium with the 2 SNPs contained mucin 1 (MUC1) and other 4 genes and SNPs significantly associated with DGC (rs2070803: P = 4.33 × 10-13; odds ratio [OR], 1.71 by meta-analysis of the studies on the 3 panels) but not with intestinal-type gastric cancer. Functional studies demonstrated that rs4072037 (P = 1.43 × 10-11; OR, 1.66 by meta-analysis) in MUC1 affects promoter activity and determines the major splicing variants of MUC1 in the gastric epithelium. Individuals that carry both SNPs rs2294008 in PSCA and rs4072037 in MUC1 have a high risk for developing DGC (OR, 8.38). Conclusions MUC1 is the second major DGC susceptibility gene identified. The SNPs rs2070803 and rs4072037 in MUC1 might be used to identify individuals at risk for this type of gastric cancer.",
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T1 - A functional single nucleotide polymorphism in mucin 1, at chromosome 1q22, determines susceptibility to diffuse-type gastric cancer

AU - Saeki, Norihisa

AU - Saito, Akira

AU - Choi, Il Ju

AU - Matsuo, Keitaro

AU - Ohnami, Sumiko

AU - Totsuka, Hirohiko

AU - Chiku, Suenori

AU - Kuchiba, Aya

AU - Lee, Yeonsu

AU - Yoon, Kyongah

AU - Kook, Myeongcherl

AU - Park, Sook Ryun

AU - Kim, Youngwoo

AU - Tanaka, Hideo

AU - Tajima, Kazuo

AU - Hirose, Hiroshi

AU - Tanioka, Fumihiko

AU - Matsuno, Yoshihiro

AU - Sugimura, Haruhiko

AU - Kato, Shunji

AU - Nakamura, Tsuneya

AU - Nishina, Tomohiro

AU - Yasui, Wataru

AU - Aoyagi, Kazuhiko

AU - Sasaki, Hiroki

AU - Yanagihara, Kazuyoshi

AU - Katai, Hitoshi

AU - Shimoda, Tadakazu

AU - Yoshida, Teruhiko

AU - Nakamura, Yusuke

AU - Hirohashi, Setsuo

AU - Sakamoto, Hiromi

PY - 2011/3

Y1 - 2011/3

N2 - Background & Aims Two major types of gastric cancer, intestinal and diffuse, develop through distinct mechanisms; the diffuse type is considered to be more influenced by genetic factors, although the mechanism is unknown. Our previous genome-wide association study associated 3 single nucleotide polymorphisms (SNPs) with diffuse-type gastric cancer (DGC); 1 was a functional SNP (rs2294008) in prostate stem cell antigen (PSCA), but the loci of the other 2 were not investigated. Methods We performed high-density mapping to explore a linkage disequilibrium status of the 2 SNPs at chromosome 1q22. A DGC case-control study was conducted using DNA from 606 cases and 1264 controls (all Japanese individuals) and validated using DNA from Japanese (304 cases, 1465 controls) and Korean (452 cases, 372 controls) individuals. The effects of SNPs on function were analyzed by reporter assays and analyses of splice variants. Results A region of a strong linkage disequilibrium with the 2 SNPs contained mucin 1 (MUC1) and other 4 genes and SNPs significantly associated with DGC (rs2070803: P = 4.33 × 10-13; odds ratio [OR], 1.71 by meta-analysis of the studies on the 3 panels) but not with intestinal-type gastric cancer. Functional studies demonstrated that rs4072037 (P = 1.43 × 10-11; OR, 1.66 by meta-analysis) in MUC1 affects promoter activity and determines the major splicing variants of MUC1 in the gastric epithelium. Individuals that carry both SNPs rs2294008 in PSCA and rs4072037 in MUC1 have a high risk for developing DGC (OR, 8.38). Conclusions MUC1 is the second major DGC susceptibility gene identified. The SNPs rs2070803 and rs4072037 in MUC1 might be used to identify individuals at risk for this type of gastric cancer.

AB - Background & Aims Two major types of gastric cancer, intestinal and diffuse, develop through distinct mechanisms; the diffuse type is considered to be more influenced by genetic factors, although the mechanism is unknown. Our previous genome-wide association study associated 3 single nucleotide polymorphisms (SNPs) with diffuse-type gastric cancer (DGC); 1 was a functional SNP (rs2294008) in prostate stem cell antigen (PSCA), but the loci of the other 2 were not investigated. Methods We performed high-density mapping to explore a linkage disequilibrium status of the 2 SNPs at chromosome 1q22. A DGC case-control study was conducted using DNA from 606 cases and 1264 controls (all Japanese individuals) and validated using DNA from Japanese (304 cases, 1465 controls) and Korean (452 cases, 372 controls) individuals. The effects of SNPs on function were analyzed by reporter assays and analyses of splice variants. Results A region of a strong linkage disequilibrium with the 2 SNPs contained mucin 1 (MUC1) and other 4 genes and SNPs significantly associated with DGC (rs2070803: P = 4.33 × 10-13; odds ratio [OR], 1.71 by meta-analysis of the studies on the 3 panels) but not with intestinal-type gastric cancer. Functional studies demonstrated that rs4072037 (P = 1.43 × 10-11; OR, 1.66 by meta-analysis) in MUC1 affects promoter activity and determines the major splicing variants of MUC1 in the gastric epithelium. Individuals that carry both SNPs rs2294008 in PSCA and rs4072037 in MUC1 have a high risk for developing DGC (OR, 8.38). Conclusions MUC1 is the second major DGC susceptibility gene identified. The SNPs rs2070803 and rs4072037 in MUC1 might be used to identify individuals at risk for this type of gastric cancer.

KW - Cancer Prevention

KW - Genome-Wide Association Study

KW - Risk Genotype

KW - Stomach Cancer

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