A genetic approach to visualization neurotechnique of multisynaptic neural pathways using plant lectin transgene

Yoshihiro Yoshihara, Takeo Mizuno, Masakiyo Nakahira, Miwa Kawasaki, Yasuyoshi Watanabe, Hiroyuki Kagamiyama, Kou ichi Jishage, Otoya Ueda, Hiroshi Suzuki, Katsuhiko Tabuchi, Kazunobu Sawamoto, Hideyuki Okano, Tetsuo Noda, Kensaku Mori

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

The wiring patterns among various types of neurons via specific synaptic connections are the basis of functional logic employed by the brain for information processing. This study introduces a powerful method of analyzing the neuronal connectivity patterns by delivering a tracer selectively to specific types of neurons while simultaneously transsynaptically labeling their target neurons. We developed a novel genetic approach introducing cDNA for a plant lectin, wheat germ agglutinin (WGA), as a transgene under the control of specific promoter elements. Using this method, we demonstrate three examples of visualization of specific transsynaptic neural pathways: the mouse cerebellar efferent pathways, the mouse olfactory pathways, and the Drosophila visual pathways. This strategy should greatly facilitate studies on the anatomical and functional organization of the developing and mature nervous system.

Original languageEnglish
Pages (from-to)33-41
Number of pages9
JournalNeuron
Volume22
Issue number1
DOIs
Publication statusPublished - 1999 Jan
Externally publishedYes

Fingerprint

Plant Lectins
Neural Pathways
Transgenes
Neurons
Efferent Pathways
Olfactory Pathways
Wheat Germ Agglutinins
Visual Pathways
Automatic Data Processing
Nervous System
Drosophila
Complementary DNA
Brain

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Yoshihara, Y., Mizuno, T., Nakahira, M., Kawasaki, M., Watanabe, Y., Kagamiyama, H., ... Mori, K. (1999). A genetic approach to visualization neurotechnique of multisynaptic neural pathways using plant lectin transgene. Neuron, 22(1), 33-41. https://doi.org/10.1016/S0896-6273(00)80676-5

A genetic approach to visualization neurotechnique of multisynaptic neural pathways using plant lectin transgene. / Yoshihara, Yoshihiro; Mizuno, Takeo; Nakahira, Masakiyo; Kawasaki, Miwa; Watanabe, Yasuyoshi; Kagamiyama, Hiroyuki; Jishage, Kou ichi; Ueda, Otoya; Suzuki, Hiroshi; Tabuchi, Katsuhiko; Sawamoto, Kazunobu; Okano, Hideyuki; Noda, Tetsuo; Mori, Kensaku.

In: Neuron, Vol. 22, No. 1, 01.1999, p. 33-41.

Research output: Contribution to journalArticle

Yoshihara, Y, Mizuno, T, Nakahira, M, Kawasaki, M, Watanabe, Y, Kagamiyama, H, Jishage, KI, Ueda, O, Suzuki, H, Tabuchi, K, Sawamoto, K, Okano, H, Noda, T & Mori, K 1999, 'A genetic approach to visualization neurotechnique of multisynaptic neural pathways using plant lectin transgene', Neuron, vol. 22, no. 1, pp. 33-41. https://doi.org/10.1016/S0896-6273(00)80676-5
Yoshihara, Yoshihiro ; Mizuno, Takeo ; Nakahira, Masakiyo ; Kawasaki, Miwa ; Watanabe, Yasuyoshi ; Kagamiyama, Hiroyuki ; Jishage, Kou ichi ; Ueda, Otoya ; Suzuki, Hiroshi ; Tabuchi, Katsuhiko ; Sawamoto, Kazunobu ; Okano, Hideyuki ; Noda, Tetsuo ; Mori, Kensaku. / A genetic approach to visualization neurotechnique of multisynaptic neural pathways using plant lectin transgene. In: Neuron. 1999 ; Vol. 22, No. 1. pp. 33-41.
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