TY - JOUR
T1 - A genetic approach to visualization neurotechnique of multisynaptic neural pathways using plant lectin transgene
AU - Yoshihara, Yoshihiro
AU - Mizuno, Takeo
AU - Nakahira, Masakiyo
AU - Kawasaki, Miwa
AU - Watanabe, Yasuyoshi
AU - Kagamiyama, Hiroyuki
AU - Jishage, Kou ichi
AU - Ueda, Otoya
AU - Suzuki, Hiroshi
AU - Tabuchi, Katsuhiko
AU - Sawamoto, Kazunobu
AU - Okano, Hideyuki
AU - Noda, Tetsuo
AU - Mori, Kensaku
N1 - Funding Information:
The authors are grateful to Richard Axel for generous support and critical comments on the manuscript. We also thank Shigetada Nakanishi, Fujio Murakami, and Kei Ito for valuable discussion; Natasha V. Reikhel and Yoshifumi Jigami for WGA cDNAs; Harry T. Orr for Pcp2 (L7) promoter; Frank L. Margolis for OMP promoter; and Chihiro Hama and Masaki Sone for Rh1–GAL4 Drosophila line. This work was supported in part by the Special Coordination Funds for Promoting Science and Technology from the Science and Technology Agency of Japan and by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan.
PY - 1999/1
Y1 - 1999/1
N2 - The wiring patterns among various types of neurons via specific synaptic connections are the basis of functional logic employed by the brain for information processing. This study introduces a powerful method of analyzing the neuronal connectivity patterns by delivering a tracer selectively to specific types of neurons while simultaneously transsynaptically labeling their target neurons. We developed a novel genetic approach introducing cDNA for a plant lectin, wheat germ agglutinin (WGA), as a transgene under the control of specific promoter elements. Using this method, we demonstrate three examples of visualization of specific transsynaptic neural pathways: the mouse cerebellar efferent pathways, the mouse olfactory pathways, and the Drosophila visual pathways. This strategy should greatly facilitate studies on the anatomical and functional organization of the developing and mature nervous system.
AB - The wiring patterns among various types of neurons via specific synaptic connections are the basis of functional logic employed by the brain for information processing. This study introduces a powerful method of analyzing the neuronal connectivity patterns by delivering a tracer selectively to specific types of neurons while simultaneously transsynaptically labeling their target neurons. We developed a novel genetic approach introducing cDNA for a plant lectin, wheat germ agglutinin (WGA), as a transgene under the control of specific promoter elements. Using this method, we demonstrate three examples of visualization of specific transsynaptic neural pathways: the mouse cerebellar efferent pathways, the mouse olfactory pathways, and the Drosophila visual pathways. This strategy should greatly facilitate studies on the anatomical and functional organization of the developing and mature nervous system.
UR - http://www.scopus.com/inward/record.url?scp=0032931430&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032931430&partnerID=8YFLogxK
U2 - 10.1016/S0896-6273(00)80676-5
DO - 10.1016/S0896-6273(00)80676-5
M3 - Article
C2 - 10027287
AN - SCOPUS:0032931430
SN - 0896-6273
VL - 22
SP - 33
EP - 41
JO - Neuron
JF - Neuron
IS - 1
ER -