A genome-wide screen reveals that the Vibrio cholerae phosphoenolpyruvate phosphotransferase system modulates virulence gene expression

Qiyao Wang, Yves A. Millet, Michael C. Chao, Jumpei Sasabe, Brigid M. Davis, Matthew K. Waldor

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Diverse environmental stimuli and a complex network of regulatory factors are known to modulate expression of Vibrio cholerae's principal virulence factors. However, there is relatively little known about how metabolic factors impinge upon the pathogen's well-characterized cascade of transcription factors that induce expression of cholera toxin and the toxin-coregulated pilus (TCP). Here, we used a transposon insertion site (TIS) sequencing-based strategy to identify new factors required for expression of tcpA, which encodes the major subunit of TCP, the organism's chief intestinal colonization factor. Besides identifying most of the genes known to modulate tcpA expression, the screen yielded ptsI and ptsH, which encode the enzyme I (EI) and Hpr components of the V. cholerae phosphoenolpyruvate phosphotransferase system (PTS). In addition to reduced expression of TcpA, strains lacking EI, Hpr, or the associated EIIA<sup>Glc</sup> protein produced less cholera toxin (CT) and had a diminished capacity to colonize the infant mouse intestine. The PTS modulates virulence gene expression by regulating expression of tcpPH and aphAB, which themselves control expression of toxT, the central activator of virulence gene expression. One mechanism by which PTS promotes virulence gene expression appears to be by modulating the amounts of intracellular cyclic AMP (cAMP). Our findings reveal that the V. cholerae PTS is an additional modulator of the ToxT regulon and demonstrate the potency of loss-of-function TIS sequencing screens for defining regulatory networks.

Original languageEnglish
Pages (from-to)3381-3395
Number of pages15
JournalInfection and Immunity
Volume83
Issue number9
DOIs
Publication statusPublished - 2015
Externally publishedYes

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Phosphoenolpyruvate
Vibrio cholerae
Virulence
Phosphotransferases
Genome
Gene Expression
Cholera Toxin
Regulon
Virulence Factors
Enzymes
Cyclic AMP
Intestines
Transcription Factors
Genes
Proteins

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

A genome-wide screen reveals that the Vibrio cholerae phosphoenolpyruvate phosphotransferase system modulates virulence gene expression. / Wang, Qiyao; Millet, Yves A.; Chao, Michael C.; Sasabe, Jumpei; Davis, Brigid M.; Waldor, Matthew K.

In: Infection and Immunity, Vol. 83, No. 9, 2015, p. 3381-3395.

Research output: Contribution to journalArticle

Wang, Qiyao ; Millet, Yves A. ; Chao, Michael C. ; Sasabe, Jumpei ; Davis, Brigid M. ; Waldor, Matthew K. / A genome-wide screen reveals that the Vibrio cholerae phosphoenolpyruvate phosphotransferase system modulates virulence gene expression. In: Infection and Immunity. 2015 ; Vol. 83, No. 9. pp. 3381-3395.
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