A Global In Vivo Drosophila RNAi Screen Identifies NOT3 as a Conserved Regulator of Heart Function

G. Gregory Neely, Keiji Kuba, Anthony Cammarato, Kazuya Isobe, Sabine Amann, Liyong Zhang, Mitsushige Murata, Lisa Elmén, Vaijayanti Gupta, Suchir Arora, Rinku Sarangi, Debasis Dan, Susumu Fujisawa, Takako Usami, Cui ping Xia, Alex C. Keene, Nakissa N. Alayari, Hiroyuki Yamakawa, Ulrich Elling, Christian Berger & 15 others Maria Novatchkova, Rubina Koglgruber, Keiichi Fukuda, Hiroshi Nishina, Mitsuaki Isobe, J. Andrew Pospisilik, Yumiko Imai, Arne Pfeufer, Andrew A. Hicks, Peter P. Pramstaller, Sai Subramaniam, Akinori Kimura, Karen Ocorr, Rolf Bodmer, Josef M. Penninger

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

Heart diseases are the most common causes of morbidity and death in humans. Using cardiac-specific RNAi-silencing in Drosophila, we knocked down 7061 evolutionarily conserved genes under conditions of stress. We present a first global roadmap of pathways potentially playing conserved roles in the cardiovascular system. One critical pathway identified was the CCR4-Not complex implicated in transcriptional and posttranscriptional regulatory mechanisms. Silencing of CCR4-Not components in adult Drosophila resulted in myofibrillar disarray and dilated cardiomyopathy. Heterozygous not3 knockout mice showed spontaneous impairment of cardiac contractility and increased susceptibility to heart failure. These heart defects were reversed via inhibition of HDACs, suggesting a mechanistic link to epigenetic chromatin remodeling. In humans, we show that a common NOT3 SNP correlates with altered cardiac QT intervals, a known cause of potentially lethal ventricular tachyarrhythmias. Thus, our functional genome-wide screen in Drosophila can identify candidates that directly translate into conserved mammalian genes involved in heart function.

Original languageEnglish
Pages (from-to)142-153
Number of pages12
JournalCell
Volume141
Issue number1
DOIs
Publication statusPublished - 2010 Apr

Fingerprint

RNA Interference
Drosophila
Genes
Critical Pathways
Chromatin Assembly and Disassembly
Dilated Cardiomyopathy
Cardiovascular System
Epigenomics
Tachycardia
Knockout Mice
Cardiovascular system
Single Nucleotide Polymorphism
Cause of Death
Heart Diseases
Heart Failure
Genome
Chromatin
Morbidity
Defects

Keywords

  • Humdisease
  • RNA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Neely, G. G., Kuba, K., Cammarato, A., Isobe, K., Amann, S., Zhang, L., ... Penninger, J. M. (2010). A Global In Vivo Drosophila RNAi Screen Identifies NOT3 as a Conserved Regulator of Heart Function. Cell, 141(1), 142-153. https://doi.org/10.1016/j.cell.2010.02.023

A Global In Vivo Drosophila RNAi Screen Identifies NOT3 as a Conserved Regulator of Heart Function. / Neely, G. Gregory; Kuba, Keiji; Cammarato, Anthony; Isobe, Kazuya; Amann, Sabine; Zhang, Liyong; Murata, Mitsushige; Elmén, Lisa; Gupta, Vaijayanti; Arora, Suchir; Sarangi, Rinku; Dan, Debasis; Fujisawa, Susumu; Usami, Takako; Xia, Cui ping; Keene, Alex C.; Alayari, Nakissa N.; Yamakawa, Hiroyuki; Elling, Ulrich; Berger, Christian; Novatchkova, Maria; Koglgruber, Rubina; Fukuda, Keiichi; Nishina, Hiroshi; Isobe, Mitsuaki; Pospisilik, J. Andrew; Imai, Yumiko; Pfeufer, Arne; Hicks, Andrew A.; Pramstaller, Peter P.; Subramaniam, Sai; Kimura, Akinori; Ocorr, Karen; Bodmer, Rolf; Penninger, Josef M.

In: Cell, Vol. 141, No. 1, 04.2010, p. 142-153.

Research output: Contribution to journalArticle

Neely, GG, Kuba, K, Cammarato, A, Isobe, K, Amann, S, Zhang, L, Murata, M, Elmén, L, Gupta, V, Arora, S, Sarangi, R, Dan, D, Fujisawa, S, Usami, T, Xia, CP, Keene, AC, Alayari, NN, Yamakawa, H, Elling, U, Berger, C, Novatchkova, M, Koglgruber, R, Fukuda, K, Nishina, H, Isobe, M, Pospisilik, JA, Imai, Y, Pfeufer, A, Hicks, AA, Pramstaller, PP, Subramaniam, S, Kimura, A, Ocorr, K, Bodmer, R & Penninger, JM 2010, 'A Global In Vivo Drosophila RNAi Screen Identifies NOT3 as a Conserved Regulator of Heart Function', Cell, vol. 141, no. 1, pp. 142-153. https://doi.org/10.1016/j.cell.2010.02.023
Neely, G. Gregory ; Kuba, Keiji ; Cammarato, Anthony ; Isobe, Kazuya ; Amann, Sabine ; Zhang, Liyong ; Murata, Mitsushige ; Elmén, Lisa ; Gupta, Vaijayanti ; Arora, Suchir ; Sarangi, Rinku ; Dan, Debasis ; Fujisawa, Susumu ; Usami, Takako ; Xia, Cui ping ; Keene, Alex C. ; Alayari, Nakissa N. ; Yamakawa, Hiroyuki ; Elling, Ulrich ; Berger, Christian ; Novatchkova, Maria ; Koglgruber, Rubina ; Fukuda, Keiichi ; Nishina, Hiroshi ; Isobe, Mitsuaki ; Pospisilik, J. Andrew ; Imai, Yumiko ; Pfeufer, Arne ; Hicks, Andrew A. ; Pramstaller, Peter P. ; Subramaniam, Sai ; Kimura, Akinori ; Ocorr, Karen ; Bodmer, Rolf ; Penninger, Josef M. / A Global In Vivo Drosophila RNAi Screen Identifies NOT3 as a Conserved Regulator of Heart Function. In: Cell. 2010 ; Vol. 141, No. 1. pp. 142-153.
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