Abstract
As a first step to understand preimplantation development, we performed global gene-expression profiling of morula and blastocyst using the NIA 15k mouse cDNA microarray. Gene expression levels were measured four times for blastocyst and five times for morula. Student's t-test at the 5% significance level identified 428 genes upregulated and 748 downregulated in blastocyst compared to morula. This trend was consistent with semi-quantative RT-PCR analysis of sample genes. The upregulated genes known to be involved in critical regulatory processes, included Mist1, Id2, Hd1, and Requiem; the downregulated genes included CREB-binding protein, Per3, zinc finger protein 217, Krox-25, and miwi1. Such well-characterized genes and many novel genes provide markers for early stages in development and starting materials for further functional studies.
| Original language | English |
|---|---|
| Journal | European Journal of Obstetrics Gynecology and Reproductive Biology |
| Volume | 115 |
| Issue number | SUPPL. |
| DOIs | |
| Publication status | Published - 2004 Jul 1 |
| Externally published | Yes |
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Keywords
- Blastocyst
- cDNA microarray
- Morula
- Preimplantation mouse embryos
ASJC Scopus subject areas
- Obstetrics and Gynaecology
- Reproductive Medicine
Cite this
A global view of gene expression in the preimplantation mouse embryo : Morula versus blastocyst. / Tanaka, Tetsuya S.; Ko, Minoru.
In: European Journal of Obstetrics Gynecology and Reproductive Biology, Vol. 115, No. SUPPL., 01.07.2004.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A global view of gene expression in the preimplantation mouse embryo
T2 - Morula versus blastocyst
AU - Tanaka, Tetsuya S.
AU - Ko, Minoru
PY - 2004/7/1
Y1 - 2004/7/1
N2 - As a first step to understand preimplantation development, we performed global gene-expression profiling of morula and blastocyst using the NIA 15k mouse cDNA microarray. Gene expression levels were measured four times for blastocyst and five times for morula. Student's t-test at the 5% significance level identified 428 genes upregulated and 748 downregulated in blastocyst compared to morula. This trend was consistent with semi-quantative RT-PCR analysis of sample genes. The upregulated genes known to be involved in critical regulatory processes, included Mist1, Id2, Hd1, and Requiem; the downregulated genes included CREB-binding protein, Per3, zinc finger protein 217, Krox-25, and miwi1. Such well-characterized genes and many novel genes provide markers for early stages in development and starting materials for further functional studies.
AB - As a first step to understand preimplantation development, we performed global gene-expression profiling of morula and blastocyst using the NIA 15k mouse cDNA microarray. Gene expression levels were measured four times for blastocyst and five times for morula. Student's t-test at the 5% significance level identified 428 genes upregulated and 748 downregulated in blastocyst compared to morula. This trend was consistent with semi-quantative RT-PCR analysis of sample genes. The upregulated genes known to be involved in critical regulatory processes, included Mist1, Id2, Hd1, and Requiem; the downregulated genes included CREB-binding protein, Per3, zinc finger protein 217, Krox-25, and miwi1. Such well-characterized genes and many novel genes provide markers for early stages in development and starting materials for further functional studies.
KW - Blastocyst
KW - cDNA microarray
KW - Morula
KW - Preimplantation mouse embryos
UR - http://www.scopus.com/inward/record.url?scp=2942514471&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2942514471&partnerID=8YFLogxK
U2 - 10.1016/j.ejogrb.2004.01.026
DO - 10.1016/j.ejogrb.2004.01.026
M3 - Article
C2 - 15196723
AN - SCOPUS:2942514471
VL - 115
JO - European Journal of Obstetrics and Gynecology and Reproductive Biology
JF - European Journal of Obstetrics and Gynecology and Reproductive Biology
SN - 0028-2243
IS - SUPPL.
ER -