Abstract
As a first step to understand preimplantation development, we performed global gene-expression profiling of morula and blastocyst using the NIA 15k mouse cDNA microarray. Gene expression levels were measured four times for blastocyst and five times for morula. Student's t-test at the 5% significance level identified 428 genes upregulated and 748 downregulated in blastocyst compared to morula. This trend was consistent with semi-quantative RT-PCR analysis of sample genes. The upregulated genes known to be involved in critical regulatory processes, included Mist1, Id2, Hd1, and Requiem; the downregulated genes included CREB-binding protein, Per3, zinc finger protein 217, Krox-25, and miwi1. Such well-characterized genes and many novel genes provide markers for early stages in development and starting materials for further functional studies.
Original language | English |
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Pages (from-to) | S85-S91 |
Journal | European Journal of Obstetrics and Gynecology and Reproductive Biology |
Volume | 115 |
Issue number | SUPPL. |
DOIs | |
Publication status | Published - 2004 Jul 1 |
Externally published | Yes |
Event | Genesis and Fate of the Preimplantation Embryo - Sorreno, Italy Duration: 2003 Sep 29 → 2003 Oct 1 |
Keywords
- Blastocyst
- Morula
- Preimplantation mouse embryos
- cDNA microarray
ASJC Scopus subject areas
- Reproductive Medicine
- Obstetrics and Gynaecology