A global view of gene expression in the preimplantation mouse embryo

Morula versus blastocyst

Tetsuya S. Tanaka, Minoru Ko

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

As a first step to understand preimplantation development, we performed global gene-expression profiling of morula and blastocyst using the NIA 15k mouse cDNA microarray. Gene expression levels were measured four times for blastocyst and five times for morula. Student's t-test at the 5% significance level identified 428 genes upregulated and 748 downregulated in blastocyst compared to morula. This trend was consistent with semi-quantative RT-PCR analysis of sample genes. The upregulated genes known to be involved in critical regulatory processes, included Mist1, Id2, Hd1, and Requiem; the downregulated genes included CREB-binding protein, Per3, zinc finger protein 217, Krox-25, and miwi1. Such well-characterized genes and many novel genes provide markers for early stages in development and starting materials for further functional studies.

Original languageEnglish
JournalEuropean Journal of Obstetrics Gynecology and Reproductive Biology
Volume115
Issue numberSUPPL.
DOIs
Publication statusPublished - 2004 Jul 1
Externally publishedYes

Fingerprint

Morula
Blastocyst
Gene Expression
Genes
Down-Regulation
CREB-Binding Protein
Zinc Fingers
Gene Expression Profiling
Oligonucleotide Array Sequence Analysis
Students
Polymerase Chain Reaction
Proteins

Keywords

  • Blastocyst
  • cDNA microarray
  • Morula
  • Preimplantation mouse embryos

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine

Cite this

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