A global view of gene expression in the preimplantation mouse embryo: Morula versus blastocyst

Tetsuya S. Tanaka, Minoru S.H. Ko

Research output: Contribution to journalConference article

31 Citations (Scopus)

Abstract

As a first step to understand preimplantation development, we performed global gene-expression profiling of morula and blastocyst using the NIA 15k mouse cDNA microarray. Gene expression levels were measured four times for blastocyst and five times for morula. Student's t-test at the 5% significance level identified 428 genes upregulated and 748 downregulated in blastocyst compared to morula. This trend was consistent with semi-quantative RT-PCR analysis of sample genes. The upregulated genes known to be involved in critical regulatory processes, included Mist1, Id2, Hd1, and Requiem; the downregulated genes included CREB-binding protein, Per3, zinc finger protein 217, Krox-25, and miwi1. Such well-characterized genes and many novel genes provide markers for early stages in development and starting materials for further functional studies.

Original languageEnglish
Pages (from-to)S85-S91
JournalEuropean Journal of Obstetrics and Gynecology and Reproductive Biology
Volume115
Issue numberSUPPL.
DOIs
Publication statusPublished - 2004 Jul 1
Externally publishedYes
EventGenesis and Fate of the Preimplantation Embryo - Sorreno, Italy
Duration: 2003 Sep 292003 Oct 1

Keywords

  • Blastocyst
  • Morula
  • Preimplantation mouse embryos
  • cDNA microarray

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology

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