A gut microbial metabolite of linoleic acid, 10-hydroxy-cis-12-octadecenoic acid, ameliorates intestinal epithelial barrier impairment partially via GPR40-MEK-ERK pathway

Junki Miyamoto, Taichi Mizukure, Si Bum Park, Shigenobu Kishino, Ikuo Kimura, Kanako Hirano, Paolo Bergamo, Mauro Rossi, Takuya Suzuki, Makoto Arita, Jun Ogawa, Soichi Tanabe

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Gut microbial metabolites of polyunsaturated fatty acids have attracted much attention because of their various physiological properties. Dysfunction of tight junction (TJ) in the intestine contributes to the pathogenesis of many disorders such as inflammatory bowel disease. We evaluated the effects of five novel gut microbial metabolites on tumor necrosis factor (TNF)-α-induced barrier impairment in Caco-2 cells and dextran sulfate sodium-induced colitis in mice. 10-Hydroxy-cis-12-octadecenoic acid (HYA), a gut microbial metabolite of linoleic acid, suppressed TNF-α and dextran sulfate sodium-induced changes in the expression of TJ-related molecules, occludin, zonula occludens-1, and myosin light chain kinase. HYA also suppressed the expression of TNF receptor 2 (TNFR2) mRNA and protein expression in Caco-2 cells and colonic tissue. In addition, HYA suppressed the protein expression of TNFR2 in murine intestinal epithelial cells. Furthermore, HYA significantly up-regulated G protein-coupled receptor (GPR) 40 expression in Caco-2 cells. It also induced [Ca2+]i responses in HEK293 cells expressing human GPR40 with higher sensitivity than linoleic acid, its metabolic precursor. The barrier-recovering effects of HYA were abrogated by a GPR40 antagonist and MEK inhibitor in Caco-2 cells. Conversely, 10-hydroxyoctadacanoic acid, which is a gut microbial metabolite of oleic acid and lacks a carbon-carbon double bond at Δ12 position, did not show these TJ-restoring activities and down-regulated GPR40 expression. Therefore, HYA modulates TNFR2 expression, at least partially, via the GPR40-MEK-ERK pathway and may be useful in the treatment of TJ-related disorders such as inflammatory bowel disease.

Original languageEnglish
Pages (from-to)2902-2918
Number of pages17
JournalJournal of Biological Chemistry
Volume290
Issue number5
DOIs
Publication statusPublished - 2015 Jan 30
Externally publishedYes

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MAP Kinase Signaling System
Mitogen-Activated Protein Kinase Kinases
Linoleic Acid
Metabolites
Tight Junctions
Caco-2 Cells
Dextran Sulfate
Tumor Necrosis Factor Receptors
Inflammatory Bowel Diseases
Carbon
Tumor Necrosis Factor-alpha
Receptors, Tumor Necrosis Factor, Type II
Occludin
Myosin-Light-Chain Kinase
HEK293 Cells
Colitis
Oleic Acid
G-Protein-Coupled Receptors
Unsaturated Fatty Acids
Intestines

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

A gut microbial metabolite of linoleic acid, 10-hydroxy-cis-12-octadecenoic acid, ameliorates intestinal epithelial barrier impairment partially via GPR40-MEK-ERK pathway. / Miyamoto, Junki; Mizukure, Taichi; Park, Si Bum; Kishino, Shigenobu; Kimura, Ikuo; Hirano, Kanako; Bergamo, Paolo; Rossi, Mauro; Suzuki, Takuya; Arita, Makoto; Ogawa, Jun; Tanabe, Soichi.

In: Journal of Biological Chemistry, Vol. 290, No. 5, 30.01.2015, p. 2902-2918.

Research output: Contribution to journalArticle

Miyamoto, J, Mizukure, T, Park, SB, Kishino, S, Kimura, I, Hirano, K, Bergamo, P, Rossi, M, Suzuki, T, Arita, M, Ogawa, J & Tanabe, S 2015, 'A gut microbial metabolite of linoleic acid, 10-hydroxy-cis-12-octadecenoic acid, ameliorates intestinal epithelial barrier impairment partially via GPR40-MEK-ERK pathway', Journal of Biological Chemistry, vol. 290, no. 5, pp. 2902-2918. https://doi.org/10.1074/jbc.M114.610733
Miyamoto, Junki ; Mizukure, Taichi ; Park, Si Bum ; Kishino, Shigenobu ; Kimura, Ikuo ; Hirano, Kanako ; Bergamo, Paolo ; Rossi, Mauro ; Suzuki, Takuya ; Arita, Makoto ; Ogawa, Jun ; Tanabe, Soichi. / A gut microbial metabolite of linoleic acid, 10-hydroxy-cis-12-octadecenoic acid, ameliorates intestinal epithelial barrier impairment partially via GPR40-MEK-ERK pathway. In: Journal of Biological Chemistry. 2015 ; Vol. 290, No. 5. pp. 2902-2918.
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AU - Park, Si Bum

AU - Kishino, Shigenobu

AU - Kimura, Ikuo

AU - Hirano, Kanako

AU - Bergamo, Paolo

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AU - Suzuki, Takuya

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AU - Tanabe, Soichi

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