A high-throughput drug-screening system targeting ABC-transporters

An application of a microfluidic gradient generator

Yuta Abe, H. Sasaki, R. Yamamoto, T. Osaki, R. Kawano, K. Kamiya, Norihisa Miki, S. Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We describe a high-throughput methodology to analyze the function of ATP binding cassette transporter (ABC-transporter) by using a microfluidic device that generates a concentration gradient of substrates. Taking advantage of the basic procedure that we established for functional analysis of ABC-transporters, we here show an advanced system utilizing multi-channel microfluidic device. We applied a microfluidic gradient generator, which makes various concentrations of substrates in a single device. After incubation of ABC-transporters at 5 different substrate concentrations in a gradient generator, we succeeded in detecting the difference of substrate transport revealed by the different fluorescence intensity from ABC-transporter vesicles.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages885-887
Number of pages3
Volume2
Publication statusPublished - 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2011 Oct 22011 Oct 6

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period11/10/211/10/6

Fingerprint

Microfluidics
Screening
Throughput
Adenosinetriphosphate
Substrates
Functional analysis
Fluorescence

Keywords

  • ABC-transporter
  • Concentration gradient
  • Microfluidics
  • PDMS

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Abe, Y., Sasaki, H., Yamamoto, R., Osaki, T., Kawano, R., Kamiya, K., ... Takeuchi, S. (2011). A high-throughput drug-screening system targeting ABC-transporters: An application of a microfluidic gradient generator. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (Vol. 2, pp. 885-887)

A high-throughput drug-screening system targeting ABC-transporters : An application of a microfluidic gradient generator. / Abe, Yuta; Sasaki, H.; Yamamoto, R.; Osaki, T.; Kawano, R.; Kamiya, K.; Miki, Norihisa; Takeuchi, S.

15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 2 2011. p. 885-887.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abe, Y, Sasaki, H, Yamamoto, R, Osaki, T, Kawano, R, Kamiya, K, Miki, N & Takeuchi, S 2011, A high-throughput drug-screening system targeting ABC-transporters: An application of a microfluidic gradient generator. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. vol. 2, pp. 885-887, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, United States, 11/10/2.
Abe Y, Sasaki H, Yamamoto R, Osaki T, Kawano R, Kamiya K et al. A high-throughput drug-screening system targeting ABC-transporters: An application of a microfluidic gradient generator. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 2. 2011. p. 885-887
Abe, Yuta ; Sasaki, H. ; Yamamoto, R. ; Osaki, T. ; Kawano, R. ; Kamiya, K. ; Miki, Norihisa ; Takeuchi, S. / A high-throughput drug-screening system targeting ABC-transporters : An application of a microfluidic gradient generator. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 2 2011. pp. 885-887
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