A high-throughput drug-screening system targeting ABC-transporters: An application of a microfluidic gradient generator

Y. Abe, H. Sasaki, R. Yamamoto, T. Osaki, R. Kawano, K. Kamiya, N. Miki, S. Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We describe a high-throughput methodology to analyze the function of ATP binding cassette transporter (ABC-transporter) by using a microfluidic device that generates a concentration gradient of substrates. Taking advantage of the basic procedure that we established for functional analysis of ABC-transporters, we here show an advanced system utilizing multi-channel microfluidic device. We applied a microfluidic gradient generator, which makes various concentrations of substrates in a single device. After incubation of ABC-transporters at 5 different substrate concentrations in a gradient generator, we succeeded in detecting the difference of substrate transport revealed by the different fluorescence intensity from ABC-transporter vesicles.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages885-887
Number of pages3
Publication statusPublished - 2011 Dec 1
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2011 Oct 22011 Oct 6

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume2

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period11/10/211/10/6

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Keywords

  • ABC-transporter
  • Concentration gradient
  • Microfluidics
  • PDMS

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Abe, Y., Sasaki, H., Yamamoto, R., Osaki, T., Kawano, R., Kamiya, K., Miki, N., & Takeuchi, S. (2011). A high-throughput drug-screening system targeting ABC-transporters: An application of a microfluidic gradient generator. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 885-887). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 2).