A human homolog of Drosophila warts tumor suppressor, h-warts, localized to mitotic apparatus and specifically phosphorylated during mitosis

Yasuyuki Nishiyama; Toru Hirota; Tetsuro Morisaki; Toshihiro Hara; Tomotoshi Marumoto; Shin Ichi Iida; Keishi Makino; Hideyuki Yamamoto; Takehisa Hiraoka; Nobuo Kitamura; Hideyuki Saya

doi:10.1016/S0014-5793(99)01224-7

A human homolog of Drosophila warts tumor suppressor, h-warts, localized to mitotic apparatus and specifically phosphorylated during mitosis

Yasuyuki Nishiyama, Toru Hirota, Tetsuro Morisaki, Toshihiro Hara, Tomotoshi Marumoto, Shin Ichi Iida, Keishi Makino, Hideyuki Yamamoto, Takehisa Hiraoka, Nobuo Kitamura, Hideyuki Saya

Research output: Contribution to journal › Article › peer-review

91 Citations (Scopus)

Abstract

We identified a human homolog of Drosophila warts tumor suppressor gene, termed h-warts, which was mapped at chromosome 6q24-25.1. The h-warts protein has a serine/threonine kinase domain and is localized to centrosomes in interphase cells. However, it becomes localized to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, in a highly dynamic manner during mitosis. Furthermore, h-warts is specifically phosphorylated in cells at mitotic phase, most likely by Cdc2 kinase. These findings suggest that h-warts functions as a component of the mitotic apparatus and is involved in proper progression of mitosis.

Original language	English
Pages (from-to)	159-165
Number of pages	7
Journal	FEBS Letters
Volume	459
Issue number	2
DOIs	https://doi.org/10.1016/S0014-5793(99)01224-7
Publication status	Published - 1999 Oct 8
Externally published	Yes

Keywords

Cdc2
Centrosome
Chromosome 6q
Midbody
Mitotic spindle
Nocodazole

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(99)01224-7

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title = "A human homolog of Drosophila warts tumor suppressor, h-warts, localized to mitotic apparatus and specifically phosphorylated during mitosis",

abstract = "We identified a human homolog of Drosophila warts tumor suppressor gene, termed h-warts, which was mapped at chromosome 6q24-25.1. The h-warts protein has a serine/threonine kinase domain and is localized to centrosomes in interphase cells. However, it becomes localized to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, in a highly dynamic manner during mitosis. Furthermore, h-warts is specifically phosphorylated in cells at mitotic phase, most likely by Cdc2 kinase. These findings suggest that h-warts functions as a component of the mitotic apparatus and is involved in proper progression of mitosis.",

keywords = "Cdc2, Centrosome, Chromosome 6q, Midbody, Mitotic spindle, Nocodazole",

author = "Yasuyuki Nishiyama and Toru Hirota and Tetsuro Morisaki and Toshihiro Hara and Tomotoshi Marumoto and Iida, {Shin Ichi} and Keishi Makino and Hideyuki Yamamoto and Takehisa Hiraoka and Nobuo Kitamura and Hideyuki Saya",

note = "Funding Information: We thank Drs. Q. Hu, and H. Maruta for providing the pCGN, and pGEX-2TH plasmids, respectively; Drs. H. Koga, N. Hanada, T. Tada, H. Nakamura and N. Mugita for valuable discussion; Dr. J. Moon for editing the manuscript; and T. Arino and K. Uriuda for secretarial assistance. This work was supported by Research Grant of the Princess Takamatsu Cancer Research Fund (#97-22906) and a grant for Cancer Research from the Ministry of Education, Science and Culture of Japan (H.S.).",

year = "1999",

month = oct,

day = "8",

doi = "10.1016/S0014-5793(99)01224-7",

language = "English",

volume = "459",

pages = "159--165",

journal = "FEBS Letters",

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T1 - A human homolog of Drosophila warts tumor suppressor, h-warts, localized to mitotic apparatus and specifically phosphorylated during mitosis

AU - Nishiyama, Yasuyuki

AU - Hirota, Toru

AU - Morisaki, Tetsuro

AU - Hara, Toshihiro

AU - Marumoto, Tomotoshi

AU - Iida, Shin Ichi

AU - Makino, Keishi

AU - Yamamoto, Hideyuki

AU - Hiraoka, Takehisa

AU - Kitamura, Nobuo

AU - Saya, Hideyuki

N1 - Funding Information: We thank Drs. Q. Hu, and H. Maruta for providing the pCGN, and pGEX-2TH plasmids, respectively; Drs. H. Koga, N. Hanada, T. Tada, H. Nakamura and N. Mugita for valuable discussion; Dr. J. Moon for editing the manuscript; and T. Arino and K. Uriuda for secretarial assistance. This work was supported by Research Grant of the Princess Takamatsu Cancer Research Fund (#97-22906) and a grant for Cancer Research from the Ministry of Education, Science and Culture of Japan (H.S.).

PY - 1999/10/8

Y1 - 1999/10/8

N2 - We identified a human homolog of Drosophila warts tumor suppressor gene, termed h-warts, which was mapped at chromosome 6q24-25.1. The h-warts protein has a serine/threonine kinase domain and is localized to centrosomes in interphase cells. However, it becomes localized to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, in a highly dynamic manner during mitosis. Furthermore, h-warts is specifically phosphorylated in cells at mitotic phase, most likely by Cdc2 kinase. These findings suggest that h-warts functions as a component of the mitotic apparatus and is involved in proper progression of mitosis.

AB - We identified a human homolog of Drosophila warts tumor suppressor gene, termed h-warts, which was mapped at chromosome 6q24-25.1. The h-warts protein has a serine/threonine kinase domain and is localized to centrosomes in interphase cells. However, it becomes localized to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, in a highly dynamic manner during mitosis. Furthermore, h-warts is specifically phosphorylated in cells at mitotic phase, most likely by Cdc2 kinase. These findings suggest that h-warts functions as a component of the mitotic apparatus and is involved in proper progression of mitosis.

KW - Cdc2

KW - Centrosome

KW - Chromosome 6q

KW - Midbody

KW - Mitotic spindle

KW - Nocodazole

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U2 - 10.1016/S0014-5793(99)01224-7

DO - 10.1016/S0014-5793(99)01224-7

M3 - Article

C2 - 10518011

AN - SCOPUS:0032863961

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JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

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A human homolog of Drosophila warts tumor suppressor, h-warts, localized to mitotic apparatus and specifically phosphorylated during mitosis

Abstract

Keywords

ASJC Scopus subject areas

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