Abstract
We identified a human homolog of Drosophila warts tumor suppressor gene, termed h-warts, which was mapped at chromosome 6q24-25.1. The h-warts protein has a serine/threonine kinase domain and is localized to centrosomes in interphase cells. However, it becomes localized to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, in a highly dynamic manner during mitosis. Furthermore, h-warts is specifically phosphorylated in cells at mitotic phase, most likely by Cdc2 kinase. These findings suggest that h-warts functions as a component of the mitotic apparatus and is involved in proper progression of mitosis.
| Original language | English |
|---|---|
| Pages (from-to) | 159-165 |
| Number of pages | 7 |
| Journal | FEBS Letters |
| Volume | 459 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1999 Oct 8 |
| Externally published | Yes |
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Keywords
- Cdc2
- Centrosome
- Chromosome 6q
- Midbody
- Mitotic spindle
- Nocodazole
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
A human homolog of Drosophila warts tumor suppressor, h-warts, localized to mitotic apparatus and specifically phosphorylated during mitosis. / Nishiyama, Yasuyuki; Hirota, Toru; Morisaki, Tetsuro; Hara, Toshihiro; Marumoto, Tomotoshi; Iida, Shin Ichi; Makino, Keishi; Yamamoto, Hideyuki; Hiraoka, Takehisa; Kitamura, Nobuo; Saya, Hideyuki.
In: FEBS Letters, Vol. 459, No. 2, 08.10.1999, p. 159-165.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A human homolog of Drosophila warts tumor suppressor, h-warts, localized to mitotic apparatus and specifically phosphorylated during mitosis
AU - Nishiyama, Yasuyuki
AU - Hirota, Toru
AU - Morisaki, Tetsuro
AU - Hara, Toshihiro
AU - Marumoto, Tomotoshi
AU - Iida, Shin Ichi
AU - Makino, Keishi
AU - Yamamoto, Hideyuki
AU - Hiraoka, Takehisa
AU - Kitamura, Nobuo
AU - Saya, Hideyuki
PY - 1999/10/8
Y1 - 1999/10/8
N2 - We identified a human homolog of Drosophila warts tumor suppressor gene, termed h-warts, which was mapped at chromosome 6q24-25.1. The h-warts protein has a serine/threonine kinase domain and is localized to centrosomes in interphase cells. However, it becomes localized to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, in a highly dynamic manner during mitosis. Furthermore, h-warts is specifically phosphorylated in cells at mitotic phase, most likely by Cdc2 kinase. These findings suggest that h-warts functions as a component of the mitotic apparatus and is involved in proper progression of mitosis.
AB - We identified a human homolog of Drosophila warts tumor suppressor gene, termed h-warts, which was mapped at chromosome 6q24-25.1. The h-warts protein has a serine/threonine kinase domain and is localized to centrosomes in interphase cells. However, it becomes localized to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, in a highly dynamic manner during mitosis. Furthermore, h-warts is specifically phosphorylated in cells at mitotic phase, most likely by Cdc2 kinase. These findings suggest that h-warts functions as a component of the mitotic apparatus and is involved in proper progression of mitosis.
KW - Cdc2
KW - Centrosome
KW - Chromosome 6q
KW - Midbody
KW - Mitotic spindle
KW - Nocodazole
UR - http://www.scopus.com/inward/record.url?scp=0032863961&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032863961&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(99)01224-7
DO - 10.1016/S0014-5793(99)01224-7
M3 - Article
C2 - 10518011
AN - SCOPUS:0032863961
VL - 459
SP - 159
EP - 165
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 2
ER -