A human IAP-family gene, Apollon, expressed in human brain cancer cells

Zhihong Chen, Mikihiko Naito, Satoko Hori, Tetsuo Mashima, Takao Yamori, Takashi Tsuruo

Research output: Contribution to journalArticle

160 Citations (Scopus)

Abstract

IAP is a family of protein that has baculovirus IAP repeat (BIR) domains and inhibits apoptosis. We found a human IAP family gene, which we named Apollon, encoding a huge protein (530 kDa) that contains a single BIR domain and a ubiquitin-conjugating enzyme domain, that is a human homolog of BRUCE. Apollon protein was expressed in four of six brain cancers (gliomas), and one of five ovarian cancers in 38 human cancer cell lines that we examined. Among the brain cancer cell lines, SNB-78 expressed a high level of Apollon, and this cell line shows resistance against various anticancer drugs. Treating SNB-78 cells with antisense oligonucleotide against Apollon reduced the expression of Apollon protein, and significantly sensitized the cells to apoptosis induced by cisplatin and camptothecin. These results suggest that Apollon protects SNB-78 cells from undergoing apoptosis and, at least in part, plays a role in tumorigenesis and drug resistance of this cell line.

Original languageEnglish
Pages (from-to)847-854
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume264
Issue number3
DOIs
Publication statusPublished - 1999 Nov 2
Externally publishedYes

Fingerprint

Brain Neoplasms
Brain
Genes
Cells
Cell Line
Apoptosis
Proteins
Ubiquitin-Conjugating Enzymes
Camptothecin
Antisense Oligonucleotides
Baculoviridae
Drug Resistance
Glioma
Pharmaceutical Preparations
Ovarian Neoplasms
Cisplatin
Carcinogenesis
Neoplasms

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

A human IAP-family gene, Apollon, expressed in human brain cancer cells. / Chen, Zhihong; Naito, Mikihiko; Hori, Satoko; Mashima, Tetsuo; Yamori, Takao; Tsuruo, Takashi.

In: Biochemical and Biophysical Research Communications, Vol. 264, No. 3, 02.11.1999, p. 847-854.

Research output: Contribution to journalArticle

Chen, Zhihong ; Naito, Mikihiko ; Hori, Satoko ; Mashima, Tetsuo ; Yamori, Takao ; Tsuruo, Takashi. / A human IAP-family gene, Apollon, expressed in human brain cancer cells. In: Biochemical and Biophysical Research Communications. 1999 ; Vol. 264, No. 3. pp. 847-854.
@article{87c5c4021c6b42b48049d8c4a23997a6,
title = "A human IAP-family gene, Apollon, expressed in human brain cancer cells",
abstract = "IAP is a family of protein that has baculovirus IAP repeat (BIR) domains and inhibits apoptosis. We found a human IAP family gene, which we named Apollon, encoding a huge protein (530 kDa) that contains a single BIR domain and a ubiquitin-conjugating enzyme domain, that is a human homolog of BRUCE. Apollon protein was expressed in four of six brain cancers (gliomas), and one of five ovarian cancers in 38 human cancer cell lines that we examined. Among the brain cancer cell lines, SNB-78 expressed a high level of Apollon, and this cell line shows resistance against various anticancer drugs. Treating SNB-78 cells with antisense oligonucleotide against Apollon reduced the expression of Apollon protein, and significantly sensitized the cells to apoptosis induced by cisplatin and camptothecin. These results suggest that Apollon protects SNB-78 cells from undergoing apoptosis and, at least in part, plays a role in tumorigenesis and drug resistance of this cell line.",
author = "Zhihong Chen and Mikihiko Naito and Satoko Hori and Tetsuo Mashima and Takao Yamori and Takashi Tsuruo",
year = "1999",
month = "11",
day = "2",
doi = "10.1006/bbrc.1999.1585",
language = "English",
volume = "264",
pages = "847--854",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - A human IAP-family gene, Apollon, expressed in human brain cancer cells

AU - Chen, Zhihong

AU - Naito, Mikihiko

AU - Hori, Satoko

AU - Mashima, Tetsuo

AU - Yamori, Takao

AU - Tsuruo, Takashi

PY - 1999/11/2

Y1 - 1999/11/2

N2 - IAP is a family of protein that has baculovirus IAP repeat (BIR) domains and inhibits apoptosis. We found a human IAP family gene, which we named Apollon, encoding a huge protein (530 kDa) that contains a single BIR domain and a ubiquitin-conjugating enzyme domain, that is a human homolog of BRUCE. Apollon protein was expressed in four of six brain cancers (gliomas), and one of five ovarian cancers in 38 human cancer cell lines that we examined. Among the brain cancer cell lines, SNB-78 expressed a high level of Apollon, and this cell line shows resistance against various anticancer drugs. Treating SNB-78 cells with antisense oligonucleotide against Apollon reduced the expression of Apollon protein, and significantly sensitized the cells to apoptosis induced by cisplatin and camptothecin. These results suggest that Apollon protects SNB-78 cells from undergoing apoptosis and, at least in part, plays a role in tumorigenesis and drug resistance of this cell line.

AB - IAP is a family of protein that has baculovirus IAP repeat (BIR) domains and inhibits apoptosis. We found a human IAP family gene, which we named Apollon, encoding a huge protein (530 kDa) that contains a single BIR domain and a ubiquitin-conjugating enzyme domain, that is a human homolog of BRUCE. Apollon protein was expressed in four of six brain cancers (gliomas), and one of five ovarian cancers in 38 human cancer cell lines that we examined. Among the brain cancer cell lines, SNB-78 expressed a high level of Apollon, and this cell line shows resistance against various anticancer drugs. Treating SNB-78 cells with antisense oligonucleotide against Apollon reduced the expression of Apollon protein, and significantly sensitized the cells to apoptosis induced by cisplatin and camptothecin. These results suggest that Apollon protects SNB-78 cells from undergoing apoptosis and, at least in part, plays a role in tumorigenesis and drug resistance of this cell line.

UR - http://www.scopus.com/inward/record.url?scp=0033517839&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033517839&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1999.1585

DO - 10.1006/bbrc.1999.1585

M3 - Article

VL - 264

SP - 847

EP - 854

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -