@article{17819b171d68466abbef486e93917862,
title = "A Human iPSC Double-Reporter System Enables Purification of Cardiac Lineage Subpopulations with Distinct Function and Drug Response Profiles",
abstract = "Wu and colleagues create a double-knockin human TBX5Clover2 and NKX2-5TagRFP reporter to delineate cardiac lineages during differentiation. Molecular and functional analyses uncover that differential expression of TBX5 and NKX2-5 specifies different cardiac fate. This reporter is applicable for studying cardiac lineage determination in vitro and isolating cardiac subpopulations.",
keywords = "CORIN, NKX2-5, TBX5, cardiomyocyte subtypes, endothelial cell lineage, epicardial lineage, hiPSC double reporter, human first and second heart field, precise drug testing, purification",
author = "Zhang, {Joe Z.} and Vittavat Termglinchan and Shao, {Ning Yi} and Ilanit Itzhaki and Chun Liu and Ning Ma and Lei Tian and Wang, {Vicky Y.} and Chang, {Alex C.Y.} and Hongchao Guo and Tomoya Kitani and Haodi Wu and Lam, {Chi Keung} and Kazuki Kodo and Nazish Sayed and Blau, {Helen M.} and Wu, {Joseph C.}",
note = "Funding Information: We thank Professor Michael Z. Lin (Department of Bioengineering, Stanford University) for providing the ASAP2 lentiviral vector. This work was supported by the American Heart Association Merit Award; Leducq Foundation 18CVD05; Burroughs Wellcome Fund 1015009 ; NIH grants R01 HL130020 , R01 HL133272 , R01 HL126527 , and R01 HL128170 (J.C.W.); R01 NS089533 (H.M.B.); K01 HL135455 (N.S.); and K99 HL133473 (H.W.); American Heart Association Postdoctoral Fellowship 16POST31150011 (H.W.); and TRDRP Fellowship 26FT-0029 (J.Z.Z). Funding Information: We thank Professor Michael Z. Lin (Department of Bioengineering, Stanford University)for providing the ASAP2 lentiviral vector. This work was supported by the American Heart Association Merit Award; Leducq Foundation 18CVD05; Burroughs Wellcome Fund 1015009; NIH grants R01 HL130020, R01 HL133272, R01 HL126527, and R01 HL128170 (J.C.W.); R01 NS089533 (H.M.B.); K01 HL135455 (N.S.); and K99 HL133473 (H.W.); American Heart Association Postdoctoral Fellowship 16POST31150011 (H.W.); and TRDRP Fellowship 26FT-0029 (J.Z.Z). J.Z.Z. conducted experiments and wrote the manuscript. V.T. K.K. T.K. and N.M. generated the reporter. I.I. performed patch-clamp experiments. N.-Y.S. and L.T. analyzed RNA-Seq data. A.C.Y.C. performed Seahorse experiments. C.L. and N.S. performed EC experiments. V.Y.W. developed MATLAB codes. H.G. H.W. C.K.L. and H.M.B. edited the manuscript. J.C.W. designed the study, wrote the manuscript, and provided funding support. J.C.W. is a co-founder of Khloris Biosciences but has no competing interests, as the work presented was performed independently. H.M.B. is a co-founder of Myoforte Therapeutics but has no competing interests, as the work presented was performed independently. Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2019",
month = may,
day = "2",
doi = "10.1016/j.stem.2019.02.015",
language = "English",
volume = "24",
pages = "802--811.e5",
journal = "Cell Stem Cell",
issn = "1934-5909",
publisher = "Cell Press",
number = "5",
}
