A Human iPSC Double-Reporter System Enables Purification of Cardiac Lineage Subpopulations with Distinct Function and Drug Response Profiles

Joe Z. Zhang, Vittavat Termglinchan, Ning Yi Shao, Ilanit Itzhaki, Chun Liu, Ning Ma, Lei Tian, Vicky Y. Wang, Alex C.Y. Chang, Hongchao Guo, Tomoya Kitani, Haodi Wu, Chi Keung Lam, Kazuki Kodo, Nazish Sayed, Helen M. Blau, Joseph C. Wu

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Wu and colleagues create a double-knockin human TBX5Clover2 and NKX2-5TagRFP reporter to delineate cardiac lineages during differentiation. Molecular and functional analyses uncover that differential expression of TBX5 and NKX2-5 specifies different cardiac fate. This reporter is applicable for studying cardiac lineage determination in vitro and isolating cardiac subpopulations.

Original languageEnglish
Pages (from-to)802-811.e5
JournalCell stem cell
Volume24
Issue number5
DOIs
Publication statusPublished - 2019 May 2

Keywords

  • CORIN
  • NKX2-5
  • TBX5
  • cardiomyocyte subtypes
  • endothelial cell lineage
  • epicardial lineage
  • hiPSC double reporter
  • human first and second heart field
  • precise drug testing
  • purification

ASJC Scopus subject areas

  • Molecular Medicine
  • Genetics
  • Cell Biology

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    Zhang, J. Z., Termglinchan, V., Shao, N. Y., Itzhaki, I., Liu, C., Ma, N., Tian, L., Wang, V. Y., Chang, A. C. Y., Guo, H., Kitani, T., Wu, H., Lam, C. K., Kodo, K., Sayed, N., Blau, H. M., & Wu, J. C. (2019). A Human iPSC Double-Reporter System Enables Purification of Cardiac Lineage Subpopulations with Distinct Function and Drug Response Profiles. Cell stem cell, 24(5), 802-811.e5. https://doi.org/10.1016/j.stem.2019.02.015