A Human iPSC Double-Reporter System Enables Purification of Cardiac Lineage Subpopulations with Distinct Function and Drug Response Profiles

Joe Z. Zhang, Vittavat Termglinchan, Ning Yi Shao, Ilanit Itzhaki, Chun Liu, Ning Ma, Lei Tian, Vicky Y. Wang, Alex C.Y. Chang, Hongchao Guo, Tomoya Kitani, Haodi Wu, Chi Keung Lam, Kazuki Kodo, Nazish Sayed, Helen M. Blau, Joseph C. Wu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Wu and colleagues create a double-knockin human TBX5 Clover2 and NKX2-5 TagRFP reporter to delineate cardiac lineages during differentiation. Molecular and functional analyses uncover that differential expression of TBX5 and NKX2-5 specifies different cardiac fate. This reporter is applicable for studying cardiac lineage determination in vitro and isolating cardiac subpopulations.

Original languageEnglish
Pages (from-to)802-811.e5
JournalCell stem cell
Volume24
Issue number5
DOIs
Publication statusPublished - 2019 May 2

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Pharmaceutical Preparations
In Vitro Techniques

Keywords

  • cardiomyocyte subtypes
  • CORIN
  • endothelial cell lineage
  • epicardial lineage
  • hiPSC double reporter
  • human first and second heart field
  • NKX2-5
  • precise drug testing
  • purification
  • TBX5

ASJC Scopus subject areas

  • Molecular Medicine
  • Genetics
  • Cell Biology

Cite this

A Human iPSC Double-Reporter System Enables Purification of Cardiac Lineage Subpopulations with Distinct Function and Drug Response Profiles. / Zhang, Joe Z.; Termglinchan, Vittavat; Shao, Ning Yi; Itzhaki, Ilanit; Liu, Chun; Ma, Ning; Tian, Lei; Wang, Vicky Y.; Chang, Alex C.Y.; Guo, Hongchao; Kitani, Tomoya; Wu, Haodi; Lam, Chi Keung; Kodo, Kazuki; Sayed, Nazish; Blau, Helen M.; Wu, Joseph C.

In: Cell stem cell, Vol. 24, No. 5, 02.05.2019, p. 802-811.e5.

Research output: Contribution to journalArticle

Zhang, JZ, Termglinchan, V, Shao, NY, Itzhaki, I, Liu, C, Ma, N, Tian, L, Wang, VY, Chang, ACY, Guo, H, Kitani, T, Wu, H, Lam, CK, Kodo, K, Sayed, N, Blau, HM & Wu, JC 2019, 'A Human iPSC Double-Reporter System Enables Purification of Cardiac Lineage Subpopulations with Distinct Function and Drug Response Profiles', Cell stem cell, vol. 24, no. 5, pp. 802-811.e5. https://doi.org/10.1016/j.stem.2019.02.015
Zhang, Joe Z. ; Termglinchan, Vittavat ; Shao, Ning Yi ; Itzhaki, Ilanit ; Liu, Chun ; Ma, Ning ; Tian, Lei ; Wang, Vicky Y. ; Chang, Alex C.Y. ; Guo, Hongchao ; Kitani, Tomoya ; Wu, Haodi ; Lam, Chi Keung ; Kodo, Kazuki ; Sayed, Nazish ; Blau, Helen M. ; Wu, Joseph C. / A Human iPSC Double-Reporter System Enables Purification of Cardiac Lineage Subpopulations with Distinct Function and Drug Response Profiles. In: Cell stem cell. 2019 ; Vol. 24, No. 5. pp. 802-811.e5.
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