A Human iPSC Double-Reporter System Enables Purification of Cardiac Lineage Subpopulations with Distinct Function and Drug Response Profiles

Joe Z. Zhang; Vittavat Termglinchan; Ning Yi Shao; Ilanit Itzhaki; Chun Liu; Ning Ma; Lei Tian; Vicky Y. Wang; Alex C.Y. Chang; Hongchao Guo; Tomoya Kitani; Haodi Wu; Chi Keung Lam; Kazuki Kodo; Nazish Sayed; Helen M. Blau; Joseph C. Wu

doi:10.1016/j.stem.2019.02.015

A Human iPSC Double-Reporter System Enables Purification of Cardiac Lineage Subpopulations with Distinct Function and Drug Response Profiles

Joe Z. Zhang, Vittavat Termglinchan, Ning Yi Shao, Ilanit Itzhaki, Chun Liu, Ning Ma, Lei Tian, Vicky Y. Wang, Alex C.Y. Chang, Hongchao Guo, Tomoya Kitani, Haodi Wu, Chi Keung Lam, Kazuki Kodo, Nazish Sayed, Helen M. Blau, Joseph C. Wu

Department of Pediatrics

Research output: Contribution to journal › Article

8 Citations (Scopus)

Abstract

Wu and colleagues create a double-knockin human TBX5^Clover2 and NKX2-5^TagRFP reporter to delineate cardiac lineages during differentiation. Molecular and functional analyses uncover that differential expression of TBX5 and NKX2-5 specifies different cardiac fate. This reporter is applicable for studying cardiac lineage determination in vitro and isolating cardiac subpopulations.

Original language	English
Pages (from-to)	802-811.e5
Journal	Cell stem cell
Volume	24
Issue number	5
DOIs	https://doi.org/10.1016/j.stem.2019.02.015
Publication status	Published - 2019 May 2

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Keywords

CORIN
NKX2-5
TBX5
cardiomyocyte subtypes
endothelial cell lineage
epicardial lineage
hiPSC double reporter
human first and second heart field
precise drug testing
purification

ASJC Scopus subject areas

Molecular Medicine
Genetics
Cell Biology

Cite this

Zhang, J. Z., Termglinchan, V., Shao, N. Y., Itzhaki, I., Liu, C., Ma, N., Tian, L., Wang, V. Y., Chang, A. C. Y., Guo, H., Kitani, T., Wu, H., Lam, C. K., Kodo, K., Sayed, N., Blau, H. M., & Wu, J. C. (2019). A Human iPSC Double-Reporter System Enables Purification of Cardiac Lineage Subpopulations with Distinct Function and Drug Response Profiles. Cell stem cell, 24(5), 802-811.e5. https://doi.org/10.1016/j.stem.2019.02.015

A Human iPSC Double-Reporter System Enables Purification of Cardiac Lineage Subpopulations with Distinct Function and Drug Response Profiles. / Zhang, Joe Z.; Termglinchan, Vittavat; Shao, Ning Yi; Itzhaki, Ilanit; Liu, Chun; Ma, Ning; Tian, Lei; Wang, Vicky Y.; Chang, Alex C.Y.; Guo, Hongchao; Kitani, Tomoya; Wu, Haodi; Lam, Chi Keung; Kodo, Kazuki; Sayed, Nazish; Blau, Helen M.; Wu, Joseph C.

In: Cell stem cell, Vol. 24, No. 5, 02.05.2019, p. 802-811.e5.

Research output: Contribution to journal › Article

Zhang, JZ, Termglinchan, V, Shao, NY, Itzhaki, I, Liu, C, Ma, N, Tian, L, Wang, VY, Chang, ACY, Guo, H, Kitani, T, Wu, H, Lam, CK, Kodo, K, Sayed, N, Blau, HM & Wu, JC 2019, 'A Human iPSC Double-Reporter System Enables Purification of Cardiac Lineage Subpopulations with Distinct Function and Drug Response Profiles', Cell stem cell, vol. 24, no. 5, pp. 802-811.e5. https://doi.org/10.1016/j.stem.2019.02.015

Zhang, Joe Z. ; Termglinchan, Vittavat ; Shao, Ning Yi ; Itzhaki, Ilanit ; Liu, Chun ; Ma, Ning ; Tian, Lei ; Wang, Vicky Y. ; Chang, Alex C.Y. ; Guo, Hongchao ; Kitani, Tomoya ; Wu, Haodi ; Lam, Chi Keung ; Kodo, Kazuki ; Sayed, Nazish ; Blau, Helen M. ; Wu, Joseph C. / A Human iPSC Double-Reporter System Enables Purification of Cardiac Lineage Subpopulations with Distinct Function and Drug Response Profiles. In: Cell stem cell. 2019 ; Vol. 24, No. 5. pp. 802-811.e5.

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abstract = "Wu and colleagues create a double-knockin human TBX5Clover2 and NKX2-5TagRFP reporter to delineate cardiac lineages during differentiation. Molecular and functional analyses uncover that differential expression of TBX5 and NKX2-5 specifies different cardiac fate. This reporter is applicable for studying cardiac lineage determination in vitro and isolating cardiac subpopulations.",

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Access to Document

10.1016/j.stem.2019.02.015