TY - JOUR
T1 - A human monoclonal antibody to carbohydrate moiety of carcinoembryonic antigen
AU - Tsukazaki, Katsumi
AU - Kuroda, Kazuhiko
AU - Mochizuki, Katsumi
AU - Kubushiro, Kaneyoshi
AU - Fukuchi, Tsuyoshi
AU - Kato, Masatoshi
AU - Hashizume, Shuichi
AU - Nozawa, Shiro
PY - 1995/1/1
Y1 - 1995/1/1
N2 - Hybridoma BSRF-S-97, secreting a human monoclonal antibody of IgG1 subclass reactive to the carcinoembryonic antigen, was generated by fusing the regional lymph node lymphocytes from a cervical cancer patient with RF-S1 human–mouse heteromyeloma fusion line. This monoclonal antibody was found specifically reactive to carinoembryonic antigen-producing cell lines, including those of cervical cancer (SKG-II), mucinous type ovarian cancer (RMUG-L), stomach cancer (MKN-45), and lung cancer (PC-10). The monoclonal antibody reactivity with pepsin- and periodate-treated carcinoembryonic antigen demonstrated that this monoclonal antibody recognizes the carbohydrate moiety of carcinoembryonic antigen specifically. Possibilities of the monoclonal antibody reaction with mucin and blood-group antigens were excluded by the comparative studies with a placental mucin-containing protein which reacted with carcinoembryonic antigen-specific rabbit polyclonal antibody. The monoclonal antibody conjugated with Pseudomonas exotoxin showed potent regression effects on the growth of the MKN-45 cell line in both the dish culture and xenografted nude mice, indicating potential usefulness of this human monoclonal antibody as a promising tumor targeting vehicle.
AB - Hybridoma BSRF-S-97, secreting a human monoclonal antibody of IgG1 subclass reactive to the carcinoembryonic antigen, was generated by fusing the regional lymph node lymphocytes from a cervical cancer patient with RF-S1 human–mouse heteromyeloma fusion line. This monoclonal antibody was found specifically reactive to carinoembryonic antigen-producing cell lines, including those of cervical cancer (SKG-II), mucinous type ovarian cancer (RMUG-L), stomach cancer (MKN-45), and lung cancer (PC-10). The monoclonal antibody reactivity with pepsin- and periodate-treated carcinoembryonic antigen demonstrated that this monoclonal antibody recognizes the carbohydrate moiety of carcinoembryonic antigen specifically. Possibilities of the monoclonal antibody reaction with mucin and blood-group antigens were excluded by the comparative studies with a placental mucin-containing protein which reacted with carcinoembryonic antigen-specific rabbit polyclonal antibody. The monoclonal antibody conjugated with Pseudomonas exotoxin showed potent regression effects on the growth of the MKN-45 cell line in both the dish culture and xenografted nude mice, indicating potential usefulness of this human monoclonal antibody as a promising tumor targeting vehicle.
KW - Carbohydrate epitope
KW - Carcinoembryonic antigen
KW - Human monoclonal antibody
KW - Immunotixin
KW - Tumor regression
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UR - http://www.scopus.com/inward/citedby.url?scp=0029416869&partnerID=8YFLogxK
U2 - 10.3233/HAB-1995-6404
DO - 10.3233/HAB-1995-6404
M3 - Article
C2 - 8688497
AN - SCOPUS:0029416869
SN - 1093-2607
VL - 6
SP - 145
EP - 152
JO - Human Antibodies
JF - Human Antibodies
IS - 4
ER -
