A human monoclonal antibody to carbohydrate moiety of carcinoembryonic antigen

Katsumi Tsukazaki, Kazuhiko Kuroda, Katsumi Mochizuki, Kaneyoshi Kubushiro, Tsuyoshi Fukuchi, Masatoshi Kato, Shuichi Hashizume, Shiro Nozawa

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Hybridoma BSRF-S-97, secreting a human monoclonal antibody of IgG1 subclass reactive to the carcinoembryonic antigen, was generated by fusing the regional lymph node lymphocytes from a cervical cancer patient with RF-S1 human–mouse heteromyeloma fusion line. This monoclonal antibody was found specifically reactive to carinoembryonic antigen-producing cell lines, including those of cervical cancer (SKG-II), mucinous type ovarian cancer (RMUG-L), stomach cancer (MKN-45), and lung cancer (PC-10). The monoclonal antibody reactivity with pepsin- and periodate-treated carcinoembryonic antigen demonstrated that this monoclonal antibody recognizes the carbohydrate moiety of carcinoembryonic antigen specifically. Possibilities of the monoclonal antibody reaction with mucin and blood-group antigens were excluded by the comparative studies with a placental mucin-containing protein which reacted with carcinoembryonic antigen-specific rabbit polyclonal antibody. The monoclonal antibody conjugated with Pseudomonas exotoxin showed potent regression effects on the growth of the MKN-45 cell line in both the dish culture and xenografted nude mice, indicating potential usefulness of this human monoclonal antibody as a promising tumor targeting vehicle.

Original languageEnglish
Pages (from-to)145-152
Number of pages8
JournalHuman Antibodies
Volume6
Issue number4
DOIs
Publication statusPublished - 1995 Jan 1

Keywords

  • Carbohydrate epitope
  • Carcinoembryonic antigen
  • Human monoclonal antibody
  • Immunotixin
  • Tumor regression

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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