A human muscle adenine nucleotide translocator gene has four exons, is located on chromosome 4, and is differentially expressed

K. Li, C. K. Warner, J. A. Hodge, S. Minoshima, Jun Kudo, R. Fukuyama, M. Maekawa, Y. Shimizu, N. Shimizu, D. C. Wallace

Research output: Contribution to journalArticle

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Abstract

The human heart-skeletal muscle adenine nucleotide translocator (ANT1) gene was isolated and sequenced. It spans 5.8 kilobases and contains four exons. The 5'-nontranscribed region contains typical CCAAT and TATA sequences, a 22-nucleotide pair inverted repeat and 13-nucleotide pair sequence homologous to a similar region in the ATP synthase β subunit gene. The region surrounding the first exon and intron is G+C-rich, and the intron contains three Sp1 binding motifs. ANT1 was assigned to chromosome 4 using both flow-sorted chromosomes and segregating human-mouse hybrid cells. Additional ANT sequences were found on at least two other chromosomes. ANT1 transcripts were present at high levels in human heart and skeletal muscle but were almost undetectable in liver, kidney, and brain. By contrast, fibroblast ANT (ANT2) mRNAs were present in all five tissues. The unique nature and arrangement of the ANT1 transcriptional control elements may account for this differential expression.

Original languageEnglish
Pages (from-to)13998-14004
Number of pages7
JournalJournal of Biological Chemistry
Volume264
Issue number24
Publication statusPublished - 1989
Externally publishedYes

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Chromosomes, Human, Pair 4
Adenine Nucleotides
Chromosomes
Introns
Muscle
Exons
Myocardium
Skeletal Muscle
Genes
Muscles
Hybrid Cells
Human Chromosomes
Nucleotides
Fibroblasts
Adenosine Triphosphate
Kidney
Liver
Messenger RNA
Brain
Tissue

ASJC Scopus subject areas

  • Biochemistry

Cite this

Li, K., Warner, C. K., Hodge, J. A., Minoshima, S., Kudo, J., Fukuyama, R., ... Wallace, D. C. (1989). A human muscle adenine nucleotide translocator gene has four exons, is located on chromosome 4, and is differentially expressed. Journal of Biological Chemistry, 264(24), 13998-14004.

A human muscle adenine nucleotide translocator gene has four exons, is located on chromosome 4, and is differentially expressed. / Li, K.; Warner, C. K.; Hodge, J. A.; Minoshima, S.; Kudo, Jun; Fukuyama, R.; Maekawa, M.; Shimizu, Y.; Shimizu, N.; Wallace, D. C.

In: Journal of Biological Chemistry, Vol. 264, No. 24, 1989, p. 13998-14004.

Research output: Contribution to journalArticle

Li, K, Warner, CK, Hodge, JA, Minoshima, S, Kudo, J, Fukuyama, R, Maekawa, M, Shimizu, Y, Shimizu, N & Wallace, DC 1989, 'A human muscle adenine nucleotide translocator gene has four exons, is located on chromosome 4, and is differentially expressed', Journal of Biological Chemistry, vol. 264, no. 24, pp. 13998-14004.
Li, K. ; Warner, C. K. ; Hodge, J. A. ; Minoshima, S. ; Kudo, Jun ; Fukuyama, R. ; Maekawa, M. ; Shimizu, Y. ; Shimizu, N. ; Wallace, D. C. / A human muscle adenine nucleotide translocator gene has four exons, is located on chromosome 4, and is differentially expressed. In: Journal of Biological Chemistry. 1989 ; Vol. 264, No. 24. pp. 13998-14004.
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AU - Kudo, Jun

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AU - Maekawa, M.

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