A kampo (traditional japanese herbal) medicine, hochuekkito, pretreatment in mice prevented influenza virus replication accompanied with GM-CSF expression and increase in several defensin MRNA levels

Katsuaki Dan, Hiroko Akiyoshi, Kaori Munakata, Hideki Hasegawa, Kenji Watanabe

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A Kampo medicine, Hochuekkito (TJ-41), with an influenza virus-preventing effect had life-extending effectiveness, and immunological responses other than interferon (IFN)-α release were examined. TJ-41 (1 g/kg) was given to C57BL/6 male mice orally once a day for 2 weeks. Mice were then intranasally infected with influenza virus. After infection, virus titers and various parameters, mRNA levels and protein expression, for immunoresponses in the bronchoalveolar lavage fluid or removed lung homogenate, were measured by plaque assay, quantitative RT-PCR and ELISA. IFN-α and -β levels of TJ-41-treated mice were higher than those of the control. Toll-like receptor TLR7 and TLR9 mRNAs were elevated after infection, but retinoic acid-inducible gene (RIG-1) family mRNA levels, RIG-1, melanoma differentiation-associated gene 5 and Leishmania G protein 2 showed no response in either TJ-41 or control groups. Interferon regulatory transcription factor (IRF)-3 mRNA levels to stimulate type I (α/β) IFN were increased, but IRF-7 did not change. Only granulocyte-macrophage colony-stimulating factor (GM-CSF) after Hochuekkito treatment was significantly elevated 2 and 3 days after infection. The mRNA levels of 7 defensins after infection increased compared to preinfection values. The key roles of TJ-41 were not only stimulation of type I IFN release but also GM-CSF-derived anti-inflammation activity. Furthermore, defensin (antimicrobial peptide) mRNA levels increased by infection and were further enhanced by TJ-41 treatment. Defensin might prevent influenza virus replication.

Original languageEnglish
Pages (from-to)314-321
Number of pages8
JournalPharmacology
Volume91
Issue number5-6
DOIs
Publication statusPublished - 2013 Jul

Fingerprint

Kampo Medicine
Defensins
Herbal Medicine
Granulocyte-Macrophage Colony-Stimulating Factor
Virus Replication
Orthomyxoviridae
Messenger RNA
Infection
Interferon Type I
Interferons
Interferon Regulatory Factor-7
T Cell Transcription Factor 1
Interferon Regulatory Factor-3
Transcription Factor 3
Toll-Like Receptors
Leishmania
Bronchoalveolar Lavage Fluid
Tretinoin
Viral Load
GTP-Binding Proteins

Keywords

  • Defensin
  • Granulocyte-macrophage colony-stimulating factor
  • Hochuekkito
  • Influenza virus

ASJC Scopus subject areas

  • Pharmacology

Cite this

A kampo (traditional japanese herbal) medicine, hochuekkito, pretreatment in mice prevented influenza virus replication accompanied with GM-CSF expression and increase in several defensin MRNA levels. / Dan, Katsuaki; Akiyoshi, Hiroko; Munakata, Kaori; Hasegawa, Hideki; Watanabe, Kenji.

In: Pharmacology, Vol. 91, No. 5-6, 07.2013, p. 314-321.

Research output: Contribution to journalArticle

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