A mathematical model of the pancreatic ductal epithelium

Y. Sohma, M. A. Gray, Y. Imai, B. E. Argent

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

A mathematical model of the HCO3--secreting pancreatic ductal epithelium was developed using network thermodynamics. With a minimal set of assumptions, the model accurately reproduced the experimentally measured membrane potentials, voltage divider ratio, transepithelial resistance and short-circuit current of nonstimulated ducts that were microperfused and bathed with a CO2/HCO3--free, HEPES-buffered solution, and also the intracellular pH of duct cells bathed in a CO2/HCO3--buffered solution. The model also accurately simulated: (i) the effect of step changes in basolateral K+ concentration, and the effect of K+ channel blockers on basolateral membrane potential; (ii) the intracellular acidification caused by a Na+-free extracellular solution and the effect of amiloride on this acidification; and (iii) the intracellular alkalinization caused by a Cl--free extracellular solution and the effect of DIDS on this alkalinization. In addition, the model predicted that the luminal Cl- conductance plays a key role in controlling both the HCO3- secretory rate and intracellular pH during HCO3- secretion. We believe that the model will be helpful in the analysis of experimental data and improve our understanding of HCO3--transporting mechanisms in pancreatic duct cells.

Original languageEnglish
Pages (from-to)53-67
Number of pages15
JournalJournal of Membrane Biology
Volume154
Issue number1
DOIs
Publication statusPublished - 1996 Dec 16

Keywords

  • Cystic fibrosis transmembrane conductance regulator
  • HCO secretion
  • Intracellular pH regulation
  • Mathematical model
  • Pancreatic duct

ASJC Scopus subject areas

  • Biophysics
  • Physiology
  • Cell Biology

Fingerprint Dive into the research topics of 'A mathematical model of the pancreatic ductal epithelium'. Together they form a unique fingerprint.

Cite this