A MEMS-based measurement system for evaluating the force-length relationship of human induced pluripotent stem cell-derived cardiomyocytes adhered on a substrate

Kenei Matsudaira, Hidetoshi Takahashi, Kayoko Hirayama-Shoji, Thanh Vinh Nguyen, Takuya Tsukagoshi, Isao Shimoyama

Research output: Contribution to journalArticle

Abstract

This paper reports on a method for evaluating the force-length relationship of adhering human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) on the substrate using a measurement system comprising of a micromachined movable plate and a piezoresistive force probe. The cells on the plate are stretched by pushing the movable plate with the piezoresistive cantilever, which is actuated by a piezo stage. The twitch forces and the applied stretch are measured quantitatively with the piezoresistive cantilever. The results demonstrated that the twitch forces of the hiPSC-CMs increased when a stretch was applied. This evaluation method improves the understanding of the intrinsic force-length relationship of hiPSC-CMs at the cellular scale.

Original languageEnglish
Article number055003
JournalJournal of Micromechanics and Microengineering
Volume29
Issue number5
DOIs
Publication statusPublished - 2019 Mar 19
Externally publishedYes

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Stem cells
MEMS
Substrates

Keywords

  • contractile force
  • force-length relationship
  • iPS-cell derived cardiomyocytes
  • MEMS
  • piezoresistive cantilever

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Mechanics of Materials
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

A MEMS-based measurement system for evaluating the force-length relationship of human induced pluripotent stem cell-derived cardiomyocytes adhered on a substrate. / Matsudaira, Kenei; Takahashi, Hidetoshi; Hirayama-Shoji, Kayoko; Nguyen, Thanh Vinh; Tsukagoshi, Takuya; Shimoyama, Isao.

In: Journal of Micromechanics and Microengineering, Vol. 29, No. 5, 055003, 19.03.2019.

Research output: Contribution to journalArticle

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