A metabologenomic approach reveals changes in the intestinal environment of mice fed on american diet

Chiharu Ishii, Yumiko Nakanishi, Shinnosuke Murakami, Ryoko Nozu, Masami Ueno, Kyoji Hioki, Wanping Aw, Akiyoshi Hirayama, Tomoyoshi Soga, Mamoru Ito, Masaru Tomita, Shinji Fukuda

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Intestinal microbiota and their metabolites are strongly associated with host physiology. Developments in DNA sequencing and mass spectrometry technologies have allowed us to obtain additional data that enhance our understanding of the interactions among microbiota, metabolites, and the host. However, the strategies used to analyze these datasets are not yet well developed. Here, we describe an original analytical strategy, metabologenomics, consisting of an integrated analysis of mass spectrometry-based metabolome data and high-throughput-sequencing-based microbiome data. Using this approach, we compared data obtained from C57BL/6J mice fed an American diet (AD), which contained higher amounts of fat and fiber, to those from mice fed control rodent diet. The feces of the AD mice contained higher amounts of butyrate and propionate, and higher relative abundances of Oscillospira and Ruminococcus. The amount of butyrate positively correlated with the abundance of these bacterial genera. Furthermore, integrated analysis of the metabolome data and the predicted metagenomic data from Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) indicated that the abundance of genes associated with butyrate metabolism positively correlated with butyrate amounts. Thus, our metabologenomic approach is expected to provide new insights and understanding of intestinal metabolic dynamics in complex microbial ecosystems.

Original languageEnglish
Article number4079
JournalInternational journal of molecular sciences
Volume19
Issue number12
DOIs
Publication statusPublished - 2018

Keywords

  • American diet
  • CE-TOFMS
  • Intestinal microbiota
  • Metabologenomics
  • Metabolome
  • Microbiome
  • Multi-omics
  • Next-generation sequencing

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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